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This work presents the development of a self-supporting aerial cable containing 144 optical fibers for 200 m spans using flexible ribbon technology fully developed in Brazil. As part of the development, standards and testing requirements were defined to adapt these cables to the Brazilian reality. Relevant information about cable design, fabrication and results of laboratory tests will also be presented. [ABSTRACT FROM AUTHOR]

Abstract: We propose a new method to project n-dimensional data onto two dimensions, for visualization purposes. Our goal is to produce a bi-dimensional representation that better separate existing clusters. Accordingly, to generate this projection we apply Differential Evolution as a meta-heuristic to optimize a divergence measure of the projected data. This divergence measure is based on the Cauchy–Schwartz divergence, extended for multiple classes. It accounts for the separability of the clusters in the projected space using the Renyi entropy and Information Theoretical Clustering analysis. We test the proposed method on two synthetic and five real world data sets, obtaining well separated projected clusters in two dimensions. These results were compared with results generated by PCA and a recent likelihood based visualization method. [Copyright &y& Elsevier]

Staining for intracellular markers with the Fix & Perm™ reagent is associated with variations in the scatter properties of leucocytes, limiting automated analysis of flow cytometry FCM data. Here, we investigated those variables significantly contributing to changes in the light scatter, autofluorescence, and bcl2 staining characteristics of peripheral blood PB leucocytes, after fixation with Fix & PermTM. Our major aim was to evaluate a new mathematical approach for automated harmonization of FCM data from datafiles corresponding to aliquots of a sample treated with cellsurfaceonly versus Fix & Perm intracellular staining techniques. Overall, neither the anticoagulant used nor sample storage for <24 h showed significant impact on the light scatter and fluorescence properties of PB leucocytes; similarly, the duration of the fixation period once >15 min were used had a minimum impact on the FCM properties of PB leucocytes. Conversely, changes in cellprotein concentrations and the fixativesample volvol ratio had a clear impact on the light scatter features of some populations of leucocytes. Accordingly, lower cellprotein concentrations were associated with lower scatter values, particularly for the neutrophils. Such changes could be partially corrected through the use of higher fixative to sample volume ratios. Despite the variable changes detected between aliquots of the same sample treated with cell surfaceonly versus intracellular staining procedures, the new mathematical approach here proposed and evaluated for automated harmonization of common parameters in both datafiles, could correct the FCM profiles of leucocytes derived from cells undergoing conventional fixationpermeabilization procedures, and made them indistinguishable from those corresponding to aliquots of the same sample treated with cellsurfaceonly staining techniques. © 2009 Clinical Cytometry Society

An efficient procedure to prepare a series of five 4amino1,1dichloro3penten2ones CHCl2COCHCMeNR1R2, where R1R2 HPh, HBn, HCH2CH2OH, CH24,CH22OCH22 from the solventfree reaction of 1,1dichloro4methoxy3penten2one with primary and secondary amines is reported. 1,1Dichloro4methoxy3penten2one was reacted with hydrazine hydrochloride, phenylhydrazine hydrochloride and semicarbazide hydrochloride to obtain 5dichloromethyl3methylpyrazoles. Aminoguanidine hydrochloride and 1,2dimethylhydrazine dihydrochloride were also reacted with 1,1dichloro4methoxy3penten2one to obtain 5dichloromethyl3methylpyrazoline and 5dichloromethyl1,2,3trimethyl pyrazolium chloride, respectively. All cyclocondensation reactions were performed in onepot procedures. J. Heterocyclic Chem., 2009.

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.

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