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BackgroundIndividuals who experience serious mental health disorders are at an increased risk of physical illness co-morbidity and early intervention is crucial. Recommendations to embed an exercise physiologist service into a mental health service have not been fully evaluated.ObjectivesThis study aimed to determine (i) demographics and clinical characteristics of the young people referred to exercise physiology, (ii) adherence to metabolic monitoring, (iii) baseline physical health and (iv) level of engagement after referral.MethodsThis is a naturalistic cohort study and included all young people referred to the exercise physiology service between 2015 and 2019 at Orygen, a specialist youth mental health service in the north-western region of Melbourne.ResultsDuring the study period of 45 months, 312 young people were referred to exercise physiology, and of those, 51.3% were male. The mean age was 19.8 years. In regard to primary diagnoses, 47.4% had a psychotic disorder and 33.7% an affective disorder. Baseline weight measurements were completed for 71.8% of young people. The proportion of young people who were classified as overweight or obese increased from 55.1% to 70.4% (p< 0.001). For those referred, 61.5% attended either an individual session or a group session. A total of 29.5% did not attend their appointment following referral.ConclusionsAs over half of young people had poor physical health at presentation, integrating an exercise physiology service into a youth mental health service is a novel and needed intervention. However, there still needs to be an emphasis on metabolic monitoring and engagement.

Background: Thousands of phacoemulsification surgeries are performed on eyes with age-related macular degeneration (AMD) complicated by choroidal neovascular membrane (CNV) in the United Kingdom each year. As populations age this number is expected to rise. Controversy over phacoemulsification’s influence on CNV activity limits the information which clinicians and these patients use to decide on surgery. This observational study aims to resolve this controversy by reporting on intravitreal injection (IVI) frequency as a pragmatic marker of CNV activity in a large cohort. Methods: A cohort of eyes with AMD complicated by CNV (n= 327) that underwent cataract surgery at a single tertiary centre from 2014 to 2019 were identified. These cases were matched by interval since CNV diagnosis at a specified ‘time zero’ within the follow-up of pseudophakic eyes with AMD (n= 327). Data concerning demographics, visual acuity (VA) and intravitreal injection frequency before and after ‘time zero’/phacoemulsification were collected. Results: Following ‘time zero’/phacoemulsification’ the mean reduction in annual IVI frequency was 0.6 injections/year (95% CI 0.4,0.9) and 0.4 injections/year (95% CI 0.1,0.7) in the comparison and phacoemulsification cohorts respectively. The mean VA gain 12 months after phacoemulsification in the intervention cohort was 11.3 (95% CI 9.2,13.4) early treatment of diabetic retinopathy study (ETDRS) letters, with 214 eyes (65.4%) having gained ≥5 ETDRS letters after surgery. Conclusions: Phacoemulsification has no clinically significant impact on the activity of pre-existent CNV secondary to AMD. Phacoemulsification should be offered to patients with AMD and cataract that limits vision, regardless of CNV activity.

The history of western New South Wales pubs is full of sorrow and tragedy, but in 1998 the area's attraction comes from having the oldest and the best preserved towns in Australia. Gold was discovered in the region in the early 1800s, and bushrangers such as the Clarke brothers and the Ben Hall gang made frequent visits to the region. They contributed to the region's economy, but also engaged in criminal activities and frequent disputes with local police.

e-ASTROGAM (‘enhanced ASTROGAM’) is a breakthrough Observatory space mission, with a detector composed by a Silicon tracker, a calorimeter, and an anticoincidence system, dedicated to the study of the non-thermal Universe in the photon energy range from 0.3 MeV to 3 GeV – the lower energy limit can be pushed to energies as low as 150 keV for the tracker, and to 30 keV for calorimetric detection. The mission is based on an advanced space-proven detector technology, with unprecedented sensitivity, angular and energy resolution, combined with polarimetric capability. Thanks to its performance in the MeV–GeV domain, substantially improving its predecessors, e-ASTROGAM will open a new window on the non-thermal Universe, making pioneering observations of the most powerful Galactic and extragalactic sources, elucidating the nature of their relativistic outflows and their effects on the surroundings. With a line sensitivity in the MeV energy range one to two orders of magnitude better than previous generation instruments, e-ASTROGAM will determine the origin of key isotopes fundamental for the understanding of supernova explosion and the chemical evolution of our Galaxy. The mission will provide unique data of significant interest to a broad astronomical community, complementary to powerful observatories such as LIGO-Virgo-GEO600-KAGRA, SKA, ALMA, E-ELT, TMT, LSST, JWST, Athena, CTA, IceCube, KM3NeT, and LISA.

The PAMELA cosmic-ray detector was launched on June 15th 2006 on board the Russian Resurs-DK1 satellite, and during ten years of nearly continuous data-taking it has observed new interesting features in cosmic rays (CRs). In a decade of operation it has provided plenty of scientific data, covering different issues related to cosmic-ray physics. Its discoveries might change our basic vision of the mechanisms of production, acceleration and propagation of cosmic rays in the Galaxy. The antimatter measurements, focus of the experiment, have set strong constraints to the nature of Dark Matter. Search for signatures of more exotic processes (such as the ones involving Strange Quark Matter) was also pursued. Furthermore, the long-term operation of the instrument had allowed a constant monitoring of the solar activity during its maximum and a detailed and prolonged study of the solar modulation, improving the comprehension of the heliosphere mechanisms. PAMELA had also measured the radiation environment around the Earth, and it detected for the first time the presence of an antiproton radiation belt surrounding our planet. The operation of Resurs-DK1 was terminated in 2016. In this article we will review the main features of the PAMELA instrument and its constructing phases. The main part of the article will be dedicated to the summary of the most relevant PAMELA results over a decade of observation.

Background:Sedentary time (ST) has been reported to have a range of negative health effects in adults, however, the evidence for such effects among children and adolescents is sparse. The primary aim of the study was to examine associations between changes in sedentary behavior (time and fragmentation) and changes in adiposity across childhood and adolescence.Methods:Participants were recruited as part of the Gateshead Millennium Study. Measures were taken at age 7 (n=502), 9 (n=506), 12 (n=420) and 15 years (n=306). Participants wore an ActiGraph GT1M and accelerometer epochs were ‘sedentary’ when recorded counts were ⩽25 counts per 15 s. ST was calculated and fragmentation (SF) was assessed by calculating the number of sedentary bouts per sedentary hour. Associations of changes in ST and SF with changes in adiposity (body mass index (BMI), and fat mass index (FMI)) were examined using bivariate linear spline models.Results:Increasing ST by 1% per year was associated with an increase in BMI of 0.08 kg m−2per year (95% CI: 0.06–0.10; P<0.001) and FMI of 0.15  kg m−2per year (0.11–0.19; P<0.001). Change in SF was associated with BMI and FMI (P<0.001). An increase of 1 bout per sedentary hour per year (that is, sedentary time becoming more fragmented) was associated with an increase in BMI of 0.07  kg m−2per year (0.06–0.09; P<0.001) and an increase in FMI of 0.14  kg m−2per year (0.10–0.18; P<0.001) over the 8 years period. However, an increase in SF between 9–12 years was associated with a 0.09  kg m−2per year decrease in BMI (−0.18–0.00; P=0.046) and 0.11  kg m−2per year decrease in FMI (−0.22–0.00; P=0.049).Conclusions:Increased ST and increased SF from 7–15 years were associated with increased adiposity. This is the first study to show age-specific associations between change in objectively measured sedentary behavior and adiposity after adjustment of moderate-to-vigorous-intensity physical activity in children and adolescents. The study suggests that, targeting sedentary behavior for obesity prevention may be most effective during periods in which we see large increases in ST.

Objective The aim of this research was to investigate the views of dental registrants in difficulty (DRiDS) on the support they received from postgraduate dental teams (PgDT) in Health Education England (HEE) and other sources. These data were complemented by the views of those appointed from the PgDT to support them on the service they provide.Method Qualitative data were collected by recording one-to-one semi structured telephone interviews, lasting approximately 30 minutes, with registrants in difficulty and supporters purposefully sampled from across England and Wales. Recordings were transcribed and the interview data analysed for recurring discourses and themes using thematic framework analysis.Setting and subjects All regional leads for DRiDs services in PgDT across the UK were asked to invite the DRiDs they were in contact with and the supporters they had appointed to contact the research team. Attempts were made to contact all who returned consent forms and six DRiDs and 11 supporters were eventually interviewed.Results Overall the DRiDs thought that the PgDT were very helpful. They were in many cases the only source of expert support and advice, particularly with regard to developing a personal development plan and collecting evidence about their practice to present to the regulator. There was a good match between the qualities that DRiDs wanted their supporters to have and the strengths supporters felt they brought to the role. The DRiDs had mixed views about the support provided by their indemnifiers and could not identify any other organisations that provided support once conditions had been imposed. Some had the support of peers; but both DRiDs and supporters felt there was a need for further support in addition to the educational support provided by PgDT and legal support provided by the indemnifier.Conclusion The DRiDS regarded the PgDT as their primary source of support and, in general, were very satisfied with the character and competence of the service.

The number of dental registrants in difficulty (DRiDs) has increased significantly in recent years and the General Dental Council or National Health Service organisations tasked with the management of dental services will, if appropriate, instruct the registrant to contact postgraduate dental teams (PgDT) based in regional offices of Health Education England and equivalent postgraduate deaneries in Wales and Scotland for assistance in meeting their conditions for continued registration. We surveyed DRiDs Leads within the PgDT with a view to understanding the current development of this important service. Results revealed that these managers had considerable relevant previous experience which underpinned their responsibility for DRiDs. Their responses indicated that there were notable differences between PgDT in the number of DRiDs seeking their help and that the development of the service and the resources deployed to help DRIDs also differed significantly. Those responsible were generally happy with the service they were providing and all were able to see DRiDs for an initial interview within four weeks of being contacted. However, weaknesses were identified such as insufficient time to support individual registrants, lack of consistent process across PgDT teams and a need for clinical training facilities.

Up until now there has been limited, contradictive data on the high energy range of the cosmic ray electron-positron, proton and helium spectra. Due to the limitations of the use of a magnetic spectrometer, over 8 years experimental data was processed using information from a sampling electro-magnetic calorimeter, a neutron detector and scintillator detectors. The use of these devices allowed us to successfully obtain the high energy cosmic ray particle spectra measurements. The results of this study clarify previous findings and greaten our understanding of the origin of cosmic rays.

An important topic of interest in imaging is the construction of protocols that are not diffraction limited. This can be achieved in a variety of ways, including classical superresolution techniques or quantum entanglement-based protocols. Here, we consider superresolving imaging in the far field using higher-order intensity correlations. We show that third- and fourth-order correlations can improve upon the first- and second-order correlations that are traditionally used in classical optics and Hanbury Brown-Twiss-type experiments. The improvement is achieved entirely by post-processing of the data. As a demonstrator, we simulate the far field intensity distribution of a circular aperture that emits thermal light and use maximum likelihood estimation to determine the radius of the aperture. We compare the achieved precision to the Cramer-Rao lower bound and find that the variance of measurements for the third- and fourth-order correlation functions are indeed closer to the Cramer-Rao bound than that of the second-order correlation function. The method presented here is general, and can be used for all kinds of incoherent emitters, geometries, and types of noise. [ABSTRACT FROM AUTHOR]

It has been suggested that in addition to genetic factors, fetal and post-natal growth influence cognition in early adulthood. However, most studies have been in developed populations, so it is unclear if the same findings would be seen in other, less developed, settings, and have used testing tools not applicable to an Australia Aboriginal population. This study investigated the relationships between cognitive function in early adulthood and birth weight and contemporary height. Simple reaction time (SRT), choice reaction time (CRT) and working memory (WM) were assessed using the CogState battery. A significant association was seen between birth weight and SRT in early adulthood, but not with the other two cognitive measures. Urban dwellers had significantly shorter SRT and CRT than their remote counterparts. Contemporary body mass index and maternal age were associated with CRT. Only fetal growth restriction was associated with WM, with greater WM in those with restricted growth. No associations were seen with contemporary height. These results suggest that fetal growth may be more important than the factors influencing post-natal growth in terms of cognition in early adulthood in this population, but that the associations may be inconsistent between cognitive outcomes. Further research is required to identify whether similar associations are seen in other, similar, populations and to assess why differences in cognitive outcome measures are seen.

The aim of this study was to evaluate safety (in terms of detecting possible adverse clinical effects attributable to vaccination), efficacy, and effects on growth performance of a gonadotropin releasing factor analog-diphtheria toxoid conjugate (commercially distributed as Improvac; Zoetis, Zaventem, Belgium) in male pigs raised in a commercial Greek farm. A total of 1,230 male pigs was enrolled in 16 weekly batches and allocated to 3 groups: barrows (castrated on the next day after birth [study Day 0]), pigs vaccinated with the above-mentioned product, and intact boars. Vaccinated pigs were injected subcutaneously with 2 mL of the anti-gonadotropin releasing factor (GnRF) vaccine at 9 to 11 wk of age (60–78 d) and 15 to 17 wk of age (102–120 d) and slaughtered at 22 to 25 wk of age (152–176 d). No clinical abnormalities or adverse events attributable to vaccination occurred. Mean BW of vaccinated pigs was 6% greater compared with barrows at slaughter (P< 0.0001). The vaccinated pigs had greater ADG than barrows from castration to slaughter (8%). In detail, a lower ADG from first to second vaccination (–12%; P< 0.0001) and a 27% greater ADG from second vaccination to slaughter (P< 0.0001) were observed. The ADG of vaccinated pigs and intact boars was not significantly different throughout the study, except from first to second vaccination (boars greater; P= 0.0059) and second vaccination to slaughter (vaccinates greater; P= 0.0390). Feed conversion ratio of barrows was 11 and 8% greater compared with vaccinated pigs (P= 0.0005) and boars (P= 0.0062) from first to second vaccination but was 23 to 26% lower compared with vaccinated pigs (P< 0.0001) and intact boars (P< 0.0001) from first vaccination to slaughter and 7 to 9.5% lower from the second vaccination to slaughter (P= 0.0029 and P= 0.0003 for vaccinates and intact boars, respectively). At slaughter, the belly fat androstenone concentration of all vaccinated pigs and 64% of intact boars was below 200 ng/g. Belly fat skatole concentration was below 20 ng/g in samples from all groups. In conclusion, vaccination against GnRF using the GnRF analog-diphtheria toxoid conjugate tested did not induce adverse clinical effects, proved effective, and compared with physical castration, resulted in greater BW at slaughter and improved ADG and feed conversion ratio from first vaccination to slaughter.

A Mixed Line Rate (MLR) optical network is a good candidate for a core backbone network because of its ability to provide diverse line rates to effectively accommodate traffic demands with heterogeneous bandwidth requirements. Because of the deleterious effects of physical impairments, there is a maximum transmission reach for optical signals before they have to be regenerated. Being expensive devices, regenerators are expected to be sparsely located and used in such a network, called a translucent optical network. In this paper, we consider the Grooming, Routing, and Wavelength Assignment (GRWA) problem so that the Quality of Transmission (QoT) for connections is satisfied, and the network-level performance metric of blocking probability is minimized. Cross-layer heuristics to effectively allocate the sparse regenerators in MLR networks are developed, and extensive simulation results are presented to demonstrate their effectiveness.

Objectives of this study were to compare fatty acid composition of subcutaneous adipose tissue of entire boars, barrows, and male pigs vaccinated against boar taint with a vaccine containing a GnRH analogue-protein conjugate (Improvac, Pfizer Animal Health) and to investigate the association between fatty acid composition and protein expression of key lipogenic enzymes in entire boars, barrows, and vaccinated pigs. Differences between groups were observed in the content of total SFA (P≤ 0.001), MUFA (P= 0.035), and n-6 PUFA (P≤ 0.001) but not n-3 PUFA (P= 0.373). Total SFA were greater (P< 0.001) in barrows and vaccinated pigs compared with entire animals. This was accompanied by an increase (P< 0.05) in the protein expression of the lipogenic enzyme fatty acid synthase in barrows and vaccinated pigs. Total MUFA content was increased (P< 0.001) in barrows compared with entire and vaccinated pigs. This was not accompanied (P> 0.05) by an increase in expression of stearoyl-CoA desaturase protein, the enzyme catalyzing MUFA biosyntheses. Total n-6 PUFA content did not differ (P< 0.001) between entire and vaccinated pigs but was lower in barrows. Expression of Δ6-desaturase protein, one of the key enzymes of PUFA biosynthesis, was greater (P< 0.05) in vaccinated pigs than in barrows but did not differ significantly between vaccinated and entire animals. We conclude that fatty acid profile of animals vaccinated against boar taint is similar to that of entire male pigs and that the effect of physical castration and vaccination on fatty acid composition involves changes in lipogenic enzyme protein expression.

ABSTRACTAvian influenza virus causes outbreaks in domestic and wild birds around the world, and sporadic human infections have been reported. A DNA vaccine encoding hemagglutinin (HA) protein from the A/Indonesia/5/05 (H5N1) strain was initially tested in two randomized phase I clinical studies. Vaccine Research Center study 304 (VRC 304) was a double-blinded study with 45 subjects randomized to placebo, 1 mg of vaccine, or 4 mg of vaccine treatment groups (n= 15/group) by intramuscular (i.m.) Biojector injection. VRC 305 was an open-label study to evaluate route, with 44 subjects randomized to intradermal (i.d.) injections of 0.5 mg by needle/syringe or by Biojector or 1 mg delivered as two 0.5-mg Biojector injections in the same deltoid or as 0.5 mg in each deltoid (n= 11/group). Injections were administered at weeks 0, 4, and 8 in both studies. Antibody responses to H5 were assessed by hemagglutination inhibition (HAI) assay, enzyme-linked immunosorbent assay (ELISA), and neutralization assay, and the H5 T cell responses were assessed by enzyme-linked immunospot and intracellular cytokine staining assays. There were no vaccine-related serious adverse events, and the vaccine was well tolerated in all groups. At 1 mg, i.d. vaccination compared to i.m. vaccination induced a greater frequency and magnitude of response by ELISA, but there were no significant differences in the frequency or magnitude of response between the i.d. and i.m. routes in the HAI or neutralization assays. T cell responses were more common in subjects who received the 1- or 4-mg dose i.m. These studies demonstrated that the DNA vaccine encoding H5 is safe and immunogenic and served to define the proper dose and route for further studies. The i.d. injection route did not offer a significant advantage over the i.m. route, and no difference was detected by delivery to one site versus splitting the dose between two sites for i.d. vaccine administration. The 4-mg dose (i.m) was further investigated in prime-boost regimens.

The core of the Zuccale low-angle normal fault contains a distinctive fault-rock zonation that developed during exhumation, composed of a diversity of fault rocks derived from lithologically heterogeneous wall rocks. Field and microstructural analyses indicate that deformation mechanisms active within the fault core, including brittle fracture, dissolution–precipitation creep and crystal-plasticity, were active broadly contemporaneously. Initially, deformation was accommodated within frictionally weak and inherently stable talc-phyllonites. Although the talc-phyllonites can account for slip at low angles, grain-scale weakening effects were limited by changes over time to the structure of the fault core, resulting from interactions with subsidiary footwall faults. Ultimately, the talc-phyllonites were dismembered into a series of isolated lenses incapable of transmitting grain-scale weakening up to the fault scale. Following this, deformation was accommodated within well-connected units of dolomite-, quartz- and calcite-bearing cataclasite, fault breccia, and foliated fault gouge. Deformation progressively migrated through this latter sequence as a result of precipitation-hardening due to the widespread growth of dolomite. The complexity of fault-zone structure, combined with changes to fault-rock distribution over time, may have resulted in fundamental changes in fault-slip behaviour, an important point to consider given the recent spectrum of slip mechanisms identified along many tectonic faults.

This paper first describes the workflow of the Pathfinder image-guided surgical robot that has been designed to replace the stereotactic frame in neurosurgery, and then details the calibration stages employed in order to achieve submillimetre positioning accuracy of a tool tip. The process uses non-linear parameter identification techniques in conjunction with some procedures for camera calibration, which exploit the fact that the camera is mounted to a calibrated robot arm that executes precise motions.

In this work the results of data analysis of the deuteron albedo radiation obtained in the PAMELA experiment are presented. PAMELA is an international space experiment carried out on board of the satellite Resurs DK-1. The high precision detectors allow to register and identify cosmic ray particles in a wide energy range. The albedo deuteron spectrum in the energy range 70 – 600 MeV/nucleon has been measured.

ABSTRACTPostoperative or posttraumatic sepsis remains one of the leading causes of morbidity and mortality in hospital populations, especially in populations in intensive care units (ICUs). Central to the successful control of sepsis-associated infections is the ability to rapidly diagnose and treat disease. The ability to identify sepsis patients before they show any symptoms would have major benefits for the health care of ICU patients. For this study, 92 ICU patients who had undergone procedures that increased the risk of developing sepsis were recruited upon admission. Blood samples were taken daily until either a clinical diagnosis of sepsis was made or until the patient was discharged from the ICU. In addition to standard clinical and laboratory parameter testing, the levels of expression of interleukin-1ß (IL-1ß), IL-6, IL-8, and IL-10, tumor necrosis factor-a, FasL, and CCL2 mRNA were also measured by real-time reverse transcriptase PCR. The results of the analysis of the data using a nonlinear technique (neural network analysis) demonstrated discernible differences prior to the onset of overt sepsis. Neural networks using cytokine and chemokine data were able to correctly predict patient outcomes in an average of 83.09% of patient cases between 4 and 1 days before clinical diagnosis with high sensitivity and selectivity (91.43% and 80.20%, respectively). The neural network also had a predictive accuracy of 94.55% when data from 22 healthy volunteers was analyzed in conjunction with the ICU patient data. Our observations from this pilot study indicate that it may be possible to predict the onset of sepsis in a mixed patient population by using a panel of just seven biomarkers.

There has been speculation that environmental exposures may be involved in the aetiology of testicular cancer in adolescent boys and young men. Indirect evidence for this hypothesis would be provided by the finding of space-time clustering. To examine this we have looked for evidence of space-time clustering using data from a population-based cancer registry from Northern England. All cases of testicular cancer diagnosed in males aged 15–24 years during the period 1968–2002 were included in the study. Tests for space-time interactions between cases were applied with fixed thresholds of close in space and close in time. Addresses at birth and diagnosis were used in the analyses. To adjust for the effect of varying population density tests were repeated replacing fixed geographical distances with nearest neighbour thresholds. A total of 257 cases of testicular cancer were identified for analysis. Overall there was no evidence for space-time clustering. However, there was statistically significant space-time clustering for 15–19-year-old based on time of birth and place of diagnosis (p<0.001). The very limited finding of space-time clustering may provide tentative evidence for an environmental, or infectious component to aetiology. However, it may well be a chance finding. A larger study based on national data is required.

Abstract: Suboptimal nutrition in early life is suggested to influence plasma glucose levels in later life. This study aimed to determine and quantify influences on plasma glucose levels at age 50. We studied 169 men and 219 women from the Newcastle Thousand Families cohort who attended for clinical examination, including measurements of fasting and 2 h post oral glucose load) at age 50. A lifecourse approach was used to estimate proportions of variance in plasma glucose levels accounted for by each stage of the lifecourse. Birth weight significantly predicted two-hour glucose levels in men (adjusted p=0.03). Body composition was a significant predictor of both glucose measures in both genders. Interactions existed between body composition and birth weight on fasting glucose in men and two-hour glucose in women and between gender and birth weight on both outcome measures. Fetal life factors directly explained little variation in either glucose measure (<2%). Adult lifestyle and body composition directly explained larger proportions of the variances (8–13%) for fasting and two-hour glucose than early life measures. The significant effect of birth weight on two-hour glucose seen in men provides support for the fetal origins hypothesis, although adult factors may be more important. Any effect of birth weight on later plasma glucose levels may be compounded by additional effects of adult body composition.

We investigate the epidemiology of 12 Verocytotoxin-producing Escherichia coli (VTEC) serogroups observed in a calf cohort on a Scottish beef farm. Fitting mathematical models to the observed time-course of infections reveals that there is significant calf-to-calf transmission of VTEC. Our models suggest that 40% of all detected infections are from calf-to-calf transmission and 60% from other sources. Variation in the rates at which infected animals recover from infection by different VTEC serogroups appears to be important. Two thirds of the observed VTEC serogroups are lost from infected calves within 1 day of infection, while the rest persist for more than 3 days. Our study has demonstrated that VTEC are transmissible between calves and are typically lost from infected animals in less than 1 week. We suggest that future field studies may wish to adopt a tighter sampling frame in order to detect all circulating VTEC serogroups in similar animal populations.

During the winter of 2000 to 2001, an outbreak due to SalmonellaEnteritidis (SE) phage type 30 (PT30), a rare strain, was detected in Canada. The ensuing investigation involved Canadian and American public health and food regulatory agencies and an academic research laboratory. Enhanced laboratory surveillance, including phage typing and pulsed-field gel electrophoresis, was used to identify cases. Case questionnaires were administered to collect information about food and environmental exposures. A case-control study with 16 matched case-control pairs was conducted to test the hypothesis of an association between raw whole almond consumption and infection. Almond samples were collected from case homes, retail outlets, and the implicated processor, and environmental samples were collected from processing equipment and associated farms for microbiological testing. One hundred sixty-eight laboratory-confirmed cases of SE PT30 infection (157 in Canada, 11 in the United States) were identified between October 2000 and July 2001. The case-control study identified raw whole almonds as the source of infection (odds ration, 21.1; 95% confidence interval, 3.6 to ∞). SE PT30 was detected in raw whole natural almonds collected from home, retail, distribution, and warehouse sources and from environmental swabs of processing equipment and associated farmers' orchards. The frequent and prolonged recovery of this specific organism from a large agricultural area was an unexpected finding and may indicate significant diffuse contamination on these farms. Identification of almonds as the source of a foodborne outbreak is a previously undocumented finding, leading to a North American recall of this product and a review of current industry practices.

The fall and recovery of dissolved oxygen (DO) is documented throughout the Gamtoos Estuary, South Africa during dry conditions and following light and heavy rainfall over a 13-month period from November 1992 to November 1993. Hypoxic conditions generally occurred in the near-bottom waters of the upper estuary. Localized fluctuations in DO levels are related to the natural diurnal fluctuation associated with photosynthesis of aquatic flora. The drop in DO levels following light rainfall is associated with the volume of oxygen-consuming compounds entering the estuary via runoff from adjacent agricultural fields. This hypoxia was short-lived. Following an extreme rainfall event, however, almost immediate hypoxia was recorded throughout the estuary, and DO levels deteriorated for some time thereafter as a result of the substantial input of organic matter into the estuary. The area of hypoxia and recovery was governed by the freshwater input at the tidal head, estuarine hydrometry and hydrodynamics. Tidal processes were identified as a source of replenishment of oxygen when, during high tide, seawater with a higher DO content penetrated the estuary.

The PAMELA experiment will be launched on-board of a polar orbiting Resurs DK1 satellite in mid-2003. The primary objective of PAMELA is to measure the flux of antiprotons (80 MeV – 190 GeV) and positrons (50 MeV – 270 GeV) in the cosmic radiation. The wide energy range and large statistics (O(104) antiprotons and O(105) positrons) will allow sensitive tests of cosmic ray propagation models and searches for exotic sources of antiparticles, such as cold dark matter neutralino annihilations. PAMELA is built around a permanent magnet spectrometer equipped with double-sided silicon detectors and surrounded by an active veto shield. This is complemented by a proportional straw tube / carbon fibre radiator transition radiation detector and a silicon-tungsten imaging calorimeter which is augmented by a scintillator shower tail catcher. A time-of-flight system provides the primary experimental trigger. The status of PAMELA is presented along with main results from simulations and test-beam studies. [Copyright &y& Elsevier]

The expansion of value-added uses for rice has created a demand for quantitative models of functional changes during postharvest handling. Consequently, this study evaluated the effects of postharvest parameters on the functional properties of long-grain (cvs. Cypress and Kaybonnet) and medium-grain (cv. Bengal) rice. The experimental treatments included rough rice drying conditions (low vs. high temperature drying), storage moisture content (10, 12, and 14%), storage temperature (4, 21, and 38°C), and storage duration (up to 36 weeks). Milling, cooking, and amylograph pasting properties were analyzed. Polynomial models (up to third-order) were developed to describe the effects of postharvest factors on the functional properties. Drying treatments, storage moisture content, and storage duration affected (P< 0.05) all of the functional properties. Storage temperature influenced (P< 0.01) cooking and pasting properties, but not milling properties. Overall, there were significant interactions among the postharvest parameters. Additionally, these factors were related to the functional properties by higher-order relationships.

Serpins are the prototypical members of the conformational disease family, a group of proteins that undergoes a change in shape that subsequently leads to tissue deposition. One specific example is alpha(1)-antichymotrypsin (ACT), which undergoes misfolding and aggregation that has been implicated in emphysema and Alzheimer's disease. In this study we have used guanidine hydrochloride (GdnHCl)-induced denaturation to investigate the conformational changes involved in the folding and unfolding of ACT. When the reaction was followed by circular dichroism spectroscopy, one stable intermediate was observed in 1.5 m GdnHCl. The same experiment monitored by fluorescence revealed a second intermediate formed in 2.5 m GdnHCl. Both these intermediates bound the hydrophobic dye ANS. These data suggest a four-state model for ACT folding N <--> I(1) <--> I(2) <--> U. I(1) and I(2) both have a similar loss of secondary structure (20%) compared with the native state. In I(2), however, there is a significant loss of tertiary interactions as revealed by changes in fluorescence emission maximum and intensity. Kinetic analysis of the unfolding reaction indicated that the native state is unstable with a fast rate of unfolding in water of 0.4 s(-1). The implications of these data for both ACT function and associated diseases are discussed.

Electrospray mass spectrometry (ES/MS), capillary-zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), and multianalyte resonant mirror are used to evaluate the heterogeneity of samples of ricin toxins extracted from five horticultural varieties of Ricinus communis seeds: R. communis zanzibariensis, carmencita, impala, sanguineus, and gibsonii. The investigation is also extended to the geographical provenance of the beans. Combining mass spectrometry, CE techniques, and resonant mirror results in a powerful analytical tool capable to characterize and differentiate between different varieties of ricin toxins. Each technique complements the others, adding another level of information. This study reveals a large extent of heterogeneity for each cultivar, demonstrating that ricin toxins consist of a series of glycosylated proteins most likely originating from a multigene family. By combining these techniques, it is possible to differentiate between zanzibariensis and the other four varieties, and that variations in the functional characteristics may be observed between the different cultivars. This study demonstrates that knowledge of the variety of R. communis beans used and their geographical provenance is essential before any type of investigation of ricin toxins is carried out. Consequently, any unusual behavior observed can only be attributed to that particular cultivar studied and not automatically extended to include all R. communis varieties. Copyright 2000 Academic Press.

The PAMELA space experiment is aimed at precise measurements of the charged light component of the cosmic ray spectrum in the energy range spanning from the sub-GeV region to the TeV region, with a particular focus on antimatter. The instrument consists of a magnetic spectrometer, an electromagnetic sampling calorimeter,a time-of-flight system, an anticoincidence shield, a tail-catcher scintillator and a neutron detector. Launched in June 2006 and hosted on the Resurs-DK1 satellite, PAMELA has been taking data for more than eight years, providing scientific results with unprecedented statistics and a continuous monitoring of the sun activity and the heliosphere.

Using the variational method, we derive simple, closed-form algebraic expressions that approximate the optimal input rms pulse width and the corresponding minimum output rms width for Gaussian pulses subject to both dispersive and nonlinear effects in single-mode fibers. We present results in both numerical and analytical forms and confirm them by the split-step Fourier numerical method. Our results cover both normal and anomalous dispersion in fibers with gain and loss. For the case of normal dispersion we show that both the optimal input and output widths are asymptotically linearly dependent on distance and dependent on the square roots of the dispersion coefficient and the transmitted power.

This work describes the development and evaluation of a multiplex polymerase chain reaction (PCR) for the detection ofBacillus anthracisstrains harbouring plasmid pX02. The multiplex also incorporated an internal control (IC) to avoid false negative reactions. Internal controls consisted of plasmids containing modified PCR target sequences, corresponding to the capC and BA813 genes ofB. anthracis, which were then co-amplified with the original target sequences using the same set of amplimers. The initial IC construct comprised of an internally deleted form of the genomic target sequence cloned into pUC19. A series of nested DNA fragments corresponding to the 23S rRNA sequences ofBacillus cereuswere then subcloned into the point of deletion, producing a number of IC constructs with similar sequences but increasing product size on PCR amplification. Neither the presence of IC DNA template or IC PCR product size affected the specificity or non-specific cross-reactivity of the original PCR assay. The concentration of IC was critical, too much IC DNA template would out compete the genomic DNA template, thus giving a false negative result. However, when the concentration of IC was optimal assay sensitivity was not compromised.

Gives details on wild turkeys. Year-round slaughtering throughout history; Modern game-management practices; Population increase in recent years; Specifics on subspecies including Easterns, Floridas, Rio Grandes and Merriams; Turkey records; Turkey call manufacturers. INSET: Turkey trivia.;Turkey records..

Recounts how bowhunter Bruce Jameson pursued a fabled giant buck for two years and finally shot the 243-pound animal.

Considers the benefits of friction calls when hunting turkeys. INSET: Choosing a call; Caring for calls.;Box calls; Slate calls....