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Background: An optimal dietary Amino Acid (AA) intake is necessary for the growth of body proteins. A new method for protein quality evaluation called Digestible Indispensable AA Score (DIAAS) was established. Despite that the information about dietary AA requirements are available for different stages of life, in practical conditions, most people deal only with the dietary proteins, if ever. Objective: The aim of this mini-review was to analyze the protein quality of protein sources by DIAAS and to find AA and protein dietary requirements in human nutrition. Methods: A literature research was performed using the keyword “amino acid”, “diet”, “nutrition”, “human”, “muscle” and “requirement” individually or all together, in Scopus, Web of Science and Pubmed. Results: The optimum amount of dietary AA is necessary for AA utilization in the body and is limited by AA which is present in the lowest amount; the surplus of other AA is not utilized in the body. Food and Agriculture Organization reported requirements for dietary protein and AA intake for infants, children, however for adults (over 18) for maintenance. Most of the studies which are dealing with the dietary AA requirements in sports nutrition are aimed at the blends of AA and for branched-chain AA. Concerning the protein quality, at this time, there is little information about the protein quality evaluated by DIAAS method. Conclusion: Dietary intake of high-quality protein or a blend of different proteins will provide all AA to the body. However, studies on protein quality evaluation by DIAAS method are necessary to perform.

Background: An optimal dietary Amino Acid (AA) intake is necessary for the growth of body proteins. A new method for protein quality evaluation called Digestible Indispensable AA Score (DIAAS) was established. Despite that the information about dietary AA requirements are available for different stages of life, in practical conditions, most people deal only with the dietary proteins, if ever. Objective: The aim of this mini-review was to analyze the protein quality of protein sources by DIAAS and to find AA and protein dietary requirements in human nutrition. Methods: A literature research was performed using the keyword “amino acid”, “diet”, “nutrition”, “human”, “muscle” and “requirement” individually or all together, in Scopus, Web of Science and Pubmed. Results: The optimum amount of dietary AA is necessary for AA utilization in the body and is limited by AA which is present in the lowest amount; the surplus of other AA is not utilized in the body. Food and Agriculture Organization reported requirements for dietary protein and AA intake for infants, children, however for adults (over 18) for maintenance. Most of the studies which are dealing with the dietary AA requirements in sports nutrition are aimed at the blends of AA and for branched-chain AA. Concerning the protein quality, at this time, there is little information about the protein quality evaluated by DIAAS method. Conclusion: Dietary intake of high-quality protein or a blend of different proteins will provide all AA to the body. However, studies on protein quality evaluation by DIAAS method are necessary to perform.

During the 70-d experimental period, 9 poly-cannulated gilts fitted with T-cannulas in the jejunum and in the cecum were fed a high-fat diet (HF; 15.5% of fat). The HF diet, which served as control (CC), was supplemented either with Lactobacillus plantarum(LP) or with inulin and horse chestnut (Aesculus Hippocastanum) (IH). On d 0, 28, 42, 56, and 70, samples of jejunal and cecal digesta were collected. As a result of diet supplementation with tested substances, lower (P< 0.05) concentrations of propionic, butyric, valeric, and caproic acids in the jejunal digesta were found in treatments LP and IH than in CC. The concentrations of lactic acid in jejunal and cecal digesta were greater (P< 0.05) in the IH treatment than in the CC or LP treatments. The concentration of butyric acid in cecal digesta in treatment CC was 7.94 mmol/L and increased (P< 0.05) to 12.57 and 11.28 mmol/L in LP and IH treatments, respectively. This represents an increase of 58 and 42%, respectively, over the CC treatment. Supplementation of a HF diet with L. plantarum, inulin, and horse chestnut resulted in greater concentrations of butyric and lactic acid in cecum.