The genus BarretonusRoudier, 1958 is a genus endemic to Macaronesia, described from the archipelago of Madeira where four species are known to date. In this study, two new species of Barretonus, B. auaritaGarcía & Oromí sp. nov. (from La Palma, Canary Islands) and B. dauteGarcía & Andújar sp. nov. (from Tenerife, Canary Islands), are described based on morphological and molecular evidence (mtCOI), and additional notes on their biology and ecology are provided. Morphology of the new species is compared with the other representatives of the genus, and their phylogenetic relationships with a Barretonusrepresentative from Madeira and species from additional genera within the Cossoninae are shown. This work increases the number of species of the genus Barretonusfrom four to six anophthalmous or microphthalmous species with an endogean lifestyle, and incorporates the Canary Islands into its Macaronesian range of distribution.