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N-Formamides are important intermediates in the synthesis of many pharmacologically active compounds and are used as protecting groups for amines or as catalysts in different reactions. The current N-formylation experiment is designed as a part of an introductory organic chemistry course for undergraduate students. The experiment includes formylation of substituted aromatic amines using formic acid under solvent-free conditions. Students are introduced to laboratory safety precautions, reaction mechanism, and basic laboratory techniques such as a solvent-free reaction setup, reflux, filtration, melting point determination, yield calculation, and lab report write-up. Students synthesized four different formamides in 50–80% yield as a straightforward application of nucleophilic substitution reactions.

Alzheimer’s disease (AD) is a progressive disease that attacks the brain’s neurons and causes problems in memory, thinking, and reasoning skills. Personalized Medicine (PM) needs a better and more accurate understanding of the relationship between human genetic data and complex diseases like AD. The goal of PM is to tailor the treatment of a case person to his individual properties. PM requires the prediction of a person’s disease from genetic data, and its success depends on the accurate detection of genetic biomarkers. Single Nucleotide polymorphisms (SNPs) are considered the most prevalent type of variation in the human genome. Epistasis has a biological relevance to complex diseases and has an important impact on PM. Detection of the most significant epistasis interactions associated with complex diseases is a big challenge. This paper reviews several machine learning techniques and algorithms to detect the most significant epistasis interactions in Alzheimer’s disease. We discuss many machine learning techniques that can be used for detecting SNPs’ combinations like Random Forests, Support Vector Machines, Multifactor Dimensionality Reduction, Neural Network, and Deep Learning. This review paper highlights the pros and cons of these techniques and explains how they can be applied in an efficient framework to apply knowledge discovery and data mining in AD disease.

Alzheimer’s disease (AD) is an irreversible, progressive disorder that assaults the nerve cells of the brain. It is the most widely recognized kind of dementia among older adults. Apolipoprotein E (APOE), is one of the most common genetic risk factors for AD whose significant association with AD is observed in various genome-wide association studies (GWAS). Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation among individuals. SNPs related to many common diseases like AD. SNPs are recognized as significant biomarkers for this disease, they help in understanding and detecting the disease in its early stages. Detecting SNPs biomarkers associated to the disease with high classification accuracy leads to early prediction and diagnosis. Machine learning techniques are utilized to discover new biomarkers of the disease. Sequential minimal optimization (SMO) algorithm with different kernels, Naive Bayes (NB), tree augmented Naive Bayes (TAN) and K2 learning algorithm have been applied on all genetic data of Alzheimer’s disease neuroimaging initiative phase 1 (ADNI-1)/Whole genome sequencing (WGS) datasets. The highest classification accuracy was achieved using 500 SNPs based on the p-value threshold (p-value <0.05). In whole genome approach ADNI-1, results revealed that NB and K2 learning algorithms scored an overall accuracy of 98% and 98.40%, respectively. In whole genome approach WGS, NB and K2 learning algorithms scored an overall accuracy of 99.63% and 99.75%, respectively.