1. Diarrhea and Colitis in Mice Require the SalmonellaPathogenicity Island 2-Encoded Secretion Function but Not SifA or Spv Effectors
- Author
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Fierer, Joshua, Okamoto, Sharon, Banerjee, Ananya, and Guiney, Donald G.
- Abstract
ABSTRACTWe investigated the roles of Salmonellapathogenicity island 2 (SPI-2) and two SPI-2 effectors in Salmonellacolitis and diarrhea in genetically resistant BALB/c.D2Slc11a1congenic mice with the wild-type Nramp1 locus. Wild-type Salmonella entericaserovar Typhimurium 14028s caused a pan-colitis, and the infected mice developed frank diarrhea with a doubling of the fecal water content. An ssaVmutant caused only a 26% increase in fecal water content, without producing the pathological changes of colitis, and it did not cause weight loss over a 1-week period of observation. However, two SPI-2 effector mutants, the spvBand sifAmutants, and a double spvB sifAmutant caused diarrhea and colitis, even though the sifAmutant was sensitive to killing by bone marrow-derived macrophages from BALB/c.D2 mice and was severely impaired in extraintestinal growth but not in growth in the cecum. These results demonstrate that systemic S. entericainfection and diarrhea/colitis are distinct pathogenic processes and that only the former requires spvBand sifA.
- Published
- 2012
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