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Human vascular endothelial cells (HUVECs) were exposed to CoCl 2as an IN VITRO model of hypoxia. Expression of VCAM-1 (vascular cell adhesion molecule), reduction of PECAM-1 (platelet endothelial cell adhesion molecule) and cytoskeletal changes without alterations in cell viability were observed. HUVECs were also exposed to ESCHERICHIA COLI lipopolysaccaride (LPS) as an IN VITRO model of inflammation: significant IL-6 release was measured. Pre-treatment of HUVECs with aescin prevented, in a concentration-dependent fashion (0.1 - 1 ?M), the action of CoCl 2on VCAM-1 and PECAM-1, also preserving endothelial cell morphology. Furthermore, aescin pre-treatment reduced IL-6 release from LPS-activated vascular endothelium. [ABSTRACT FROM AUTHOR]