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Microscopy and histology techniques can be applied to morphological study of fungi and bacteria contaminating ancient human osteological remains. Undecalcified samples are cut with a diamond rotary blade microtome and an original technique was applied using adhesive tape to prevent damage to the bone surface during sectioning. We used light microscopy, polarized light microscopy, and epi-illumination fluorescence systems. Common dyes can be applied to 80 μm sections using classic staining techniques to reveal the architecture of bone and the presence of infecting biological agents.

On 109 male Sherpas, aged 18–85 years, 24 anthropometric measurements were performed, including weight, height, 18 bony measurements, 2 limb circumferences, and 4 skinfolds. From these data, upper and lower limb lengths, 12 indices, and total body fat were calculated. The Sherpas are of medium stature and body build, with Mongoloid cranial and facial characteristics and very little body fat. Their relatively large chests, long legs, and large calf muscles may be a racial adaptation to the high altitude at which they live and to the load-carrying, which is a feature of their life.