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MOF (MYST1, KAT8) is the major H4K16 lysine acetyltransferase (KAT) in Drosophila and mammals and is essential for embryonic development. However, little is known regarding the role of MOF in specific cell lineages. Here we analyze the differential role of MOF in proliferating and terminally differentiated tissues at steady state and under stress conditions. In proliferating cells, MOF directly binds and maintains the expression of genes required for cell cycle progression. In contrast, MOF is dispensable for terminally differentiated, postmitotic glomerular podocytes under physiological conditions. However, in response to injury, MOF is absolutely critical for podocyte maintenance in vivo. Consistently, we detect defective nuclear, endoplasmic reticulum and Golgi structures, as well as presence of multivesicular bodies in vivo in podocytes lacking Mof following injury. Undertaking genome-wide expression analysis of podocytes, we uncover several MOF-regulated pathways required for stress response. We find that MOF, along with the members of the non-specific lethal but not the male-specific lethal complex, directly binds to genes encoding the lysosome, endocytosis and vacuole pathways, which are known regulators of podocyte maintenance. Thus, our work identifies MOF as a key regulator of cellular stress response in glomerular podocytes.

We report on our results from a fully relativistic simulation of the quenched bottomonium spectrum. Using an anisotropic formulation of Lattice QCD, we were able to retain a very fine resolution into the temporal direction for a range of different lattice spacings. At fixed renormalized anisotropy we study the scaling properties of the spectrum and compare our results with non-relativistic calculations. [Copyright &y& Elsevier]

We report results from full QCD calculations with two flavors of dynamical staggered fermions on anisotropic lattices. The physical anisotropy as determined from spatial and temporal masses, their corresponding dispersion relations, and spatial and temporal Wilson loops is studied as a function of the bare gauge anisotropy and the bare velocity of light appearing in the Dirac operator. The anisotropy dependence of staggered fermion flavor symmetry breaking is also examined. These results will then be applied to the study of 2-flavor QCD thermodynamics. [Copyright &y& Elsevier]