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The present study aimed to compare the dynamics of growth of various chicken genotypes exposed to heat stress, low-input diets, and free-range farming by using Gompertz model to gain insights into their capabilities to face environmental and nutritional challenges. Three in vivotrials (T1: heat stress, T2: low-input diets, and T3: free-range system) were conducted, involving a total of 671 animals. Five chicken genotypes were employed in each trial: 2 Italian local breeds, Bionda Piemontese (BP) and Robusta Maculata (RM), along with their crossbreeds with Sasso hens (BP×SAand RM×SA), and a commercial hybrid (Ross 308). One-day-old male chicks were individually identified, and the 5 genotypes were randomly allocated to different challenging conditions: T1 involved 2 environmental temperatures (thermoneutral vs. high temperature); T2 involved 2 diets (standard vs. low-input); T3 involved 2 rearing systems (conventional vs. free-range). The chickens were weighed once a week from their arrival until slaughtering, and the data were used to build growth curves using the Gompertz model. Chickens from different genotypes were slaughtered at varying ages based on their maturity. In all trials, the challenging conditions significantly reduced adult body weight (A; −31.0%) and maximum growth rate (MGR; −25.6%) of Ross chickens. In contrast, in T1 and T2, no significant changes were observed in the main growth curve parameters of local breeds and crossbreeds, while under free-range conditions, there was even an increase in the A and MGR of these genotypes. The crossbreeding was effective in increasing A and MGR of BP (+30.5% in BP×SA), as well as in improving the precocity and MGR of RM (+19.5% in RM×SA). Our findings highlight the effectiveness of the Gompertz model as a tool for evaluating birds’ adaptability and confirm the greater ability of local breeds and crossbreeds to adapt to different challenges. In conclusion, our methodological approach could be used to choose the genotype most suited to the environmental context and confirm the potential advantages of crossbreeding for enhancing resilience and sustainability.

Recent foodborne pathogen outbreaks associated with dry pet food and treats have focused the attention on these products as vehicles of pathogens for both pets and their owners. This study investigates the purchasing habits of dog owners and determines if and in what form pet snacks can be potentially dangerous for humans, especially for children. For these purposes, questionnaires collected from 406 dog owners were evaluated and microbiological analyses were performed on 120 dry pet snacks. The shape of the pet snack affects the purchase (median score, Mdn = 6.4) and the star bone was the preferred one (Mdn = 7.1). Most of the participants (76.0%; p< 0.001) stated that snacks are attractive to their children and that the possibility for children to get in contact with pet snacks is rare, but not implausible (Mdn = 3.1). Indeed, more than 6% of respondents admitted that there had been incidents of involuntary ingestion of pet snacks, with fever and gastrointestinal manifestations. Microbiological analyses showed that 119 dry pet snacks analyzed of a total of 120 (99.2%) were in good sanitary condition except for one sample where the presence of Listeria ivanoviiwas confirmed. The upward trend in the presence of pets in households and the strong and continuous growth in value and volume of the pet food market led researchers to analyze possible public health issues. Children and infants in particular are the most exposed subjects as they are more likely to come into contact with pet snacks, attracted by a shape similar to that of a toy. For this reason, although our findings did not highlight important microbiological contamination of pet snacks, it would be useful to standardize food safety criteria with those for human food from a One Health perspective.

AbstractMovements of livestock across the Member States is monitored using the Trade Control and Expert System (TRACES), a web-based veterinarian certification tool. This study aimed to evaluate the criticalities of this tracing system and the most frequent non-conformities in their compilation. TRACES of cattle (n = 320) departed from France and arriving or transiting across Puglia (Italy) in 2019 (n = 199) and 2020 (n = 121) were analysed. The distribution arriving in Italy and Greece did not vary between the 2 years. The majority of the bovine (53.4%) were medium-sized cattle transported for fattening with a median body weight of 410 kg. However, almost half of the TRACES did not report correctly the total expected loaded weight or the available space, so for them, it was impossible to calculate and double-check whether the minimal space allowance was respected. Long journeys must include a resting stop after the first 14 h, however, 20.9% of the examined and declared stops did not comply with it. Finally, comparing the expected declared journey duration with a calculated one (dividing travel distance by an average speed of 70 km/h, adding 1 h for loading and unloading, and 1 h or 24 h for resting stops), we found that only 30.6% of the journey durations were congruent. The irregularities resulted associated with consignors and transporters (p < .05). Overall, the proportion of mistakes and non-compliance suggest that there is a need to improve the current practice to enhance animal welfare during transportation.HIGHLIGHTSTRACES (n = 320) of cattle transported from France in 2019 and 2022 were analysed.Almost half of the TRACES were not filled correctly.TRACES should be implemented and double-checked carefully to ensure animal welfare.

AbstractLimited information is available to evaluate optimal growth in Toy Poodles. This work aimed at comparing three growth curves, proposing centile charts and developing a model to estimate the adult body weight (BW) in Toy Poodles. A total of 65 puppies (male = 30, female = 35) born in the same breeding centre were used. BW at birth and, weekly BW, for 53 weeks, BW of parents, litter size, type of management, daily activity and neutering were recorded. Forty-six puppies were sold, and their data were reported by the new owners. Three growth curves (i.e. Hawthorne, Brody and Gompertz) were constructed and compared; Linear Mixed Models including demographic characteristics and management habits were built. The BW at birth was 154 ± 35 g and adult BW was 3208 ± 860 g. Based on the goodness-of-fit and accuracy indices, Gompertz was the best growth model and was selected to plot centile curves based on sex. Toy Poodles achieved 50% of their adult weight at 11–12 weeks, with an overall growth rate of 11.8%. Adult BW was affected by birth BW (p < .01), sex (p < .05) and mother’s BW (p < .01) and their effects varied depending on the age. Extrinsic factors, including litter size, type of management and daily activity were less significant, probably due to the standardised and high-level management of these Toy Poodles. These new and applicable tools for monitoring the growth and predicting adult BW could be useful for veterinarians, breeders and owners for early diagnosis of poor health and welfare. Subject classification codes:companion animals sectionsHighlightsPerformance of three logistic models for describing the growth curve in Italian Toy Poodles were comparedBased on the goodness of fit and accuracy indices, Gompertz was the best growth modelThe centile growth curves were constructed for males and females using the GompertzAdult body weight (BW) was mainly affected by the sex and birth BW, and less by BW of the parents and litter sizeMonitoring BW of puppies may be useful to enhance their health and welfare

The majority of poultry meat used to be sourced from intensively housed birds. However, consumer preference has since demanded poultry producers develop more sustainable farming systems. Although free-range farming is considered beneficial for animal welfare, it is not as easy to standardize as an intensive system, which makes the choice of bird genotype appear crucial for alternative systems. In this study, we aimed to evaluate the effect of conventional and free-range rearing systems on the immune status, stress parameters, intestinal morphology and mortality in commercial hybrids (Ross 308) and local poultry strains, Bionda Piemontese (BP), Robusta Maculata (RM), BP x Sasso (BPxS), and RM x Sasso (RMxS). RNA was extracted from the jejunum and spleen to assess the mRNA expression of IL-2, IL-6, IL-10, IL-18, IL-1β, inducible nitric oxide synthase (iNOS), toll-like receptor (TLR)-4, and interferon gamma (IFN-γ). The heterophil:lymphocyte (H/L) ratio and intestinal histomorphometric evaluation were also calculated. We found that compared to the conventional system, the rearing system significantly affected the jejunum expression of IL-10, iNOS, IL-2, and IL-6, where these genes were upregulated in free-range system. A significant interaction between the rearing system and the genotype was also shown. More specifically, local breeds showed a significantly higher expression (P< 0.001) of IL-6in the free-range system compared to the same genotypes in the conventional system. Moreover, IL-6is constantly upregulated in local breeds within the free-range system compared to Ross hybrids. We also found significantly increased H/L and mortality rates in the latter, compared to the local breeds in the free-range reared system. The jejunum morphology also demonstrated a significantly higher villus height in BP and BPxS compared to the Ross hybrids. Overall, the results of our study confirm that the intense selection for growth in broiler chickens may have reduced their ability to react to the environmental stimuli related to free-range systems, resulting in a lower adaptability to a free-range environment, thus making them inappropriate for any farming system other than the conventional one. On the contrary, local chicken breeds are able to adapt and survive in the free-range system of rearing, and represent a genetic resource especially when adaptability to free-range conditions is required.

Rabbit breeding has many critical aspects related to reproduction, production, and animal welfare, which reduce its profitability as well as consumer attractiveness. Dietary supplementation with n-3 polyunsaturated fatty acids (PUFA) seems to be a good nutritional strategy to improve several aspects of rabbit breeding, enhance animal welfare and produce a new functional food considered healthy for human consumption. For this reason, the main available scientific research regarding the physiological effects of n-3 PUFA rich products supplemented to the rabbit diet will be reviewed. In particular, consequences on the reproductive performances of both doe and buck, the productive parameters, and the meat quality will be analysed.

The present study aims to validate the Gompertz model to predict the growth performance of chicken crosses according to growth curve parameters of the parental lines and the estimated heterosis for each curve parameter. A total of 252 one-day-old chicks of both sexes belonging to six genotypes, including Ross 308, Sassò (SA), Bionda Piemontese (BP), and Robusta Maculata (RM), and the crosses between these local breeds and SA (BP × SA and RM × SA) were randomly allocated in 18 pens (3 pens/genotype) in mixed-sex groups (14 animals/pen; 7 females and 7 males). The individual body weight (BW) of all birds was recorded once a week from hatching until slaughtering (81 d for Ross 308; 112 d for SA, 140 d for the other genotypes). We drew up our final dataset with 240 birds (40 birds/genotype; 20 females and 20 males). The growth curve of each genotype was described using the Gompertz model, and the heterosis for each growth curve parameter was calculated as the difference between F1crosses and the average of parental breeds. The predicted growth curve parameters were evaluated by cross-validation. The Gompertz model accurately estimated the growth curves of all the genotypes (R2>0.90). Heterosis was significant for almost all growth curve parameters in both crosses (P<0.05). Heterosis ranged from -13.0% to +11.5%, depending on parameters, but varied slightly between the crossbreeds (BP × SA and RM × SA). The predicted values of adult BW, weight at the inflection point, and maximum growth rate were overestimated for BP × SA and underestimated for RM × SA, with a mean error between observed and predicted values < │2.7│% for all the curve parameters. In conclusion, the growth performance of chicken crosses between local breeds and commercial strains can be accurately predicted with Gompertz parameters of the parental lines adjusting for heterosis.

The aim of this study was to analyze the relationship between the kinetic behavior, carcass characteristics, oxidative status (blood and meat), and meat fatty acid profiles of 6 organically reared slower growing chicken genotypes (SrG). One hundred male chickens of 6 SrG were used: Ranger Classic (RC), Ranger Gold (RG), Rowan Ranger (RR), RedJA (RJ), CY Gen 5 JA87 (CY), and M22 × JA87 (M). Twenty chickens/genotype were selected to analyze behavior, while, 15 individuals were slaughtered and different traits were analyzed in the blood and drumstick meat. The variables were grouped into different principal components: kinetic activity (PC1, with explorative attitude as the highest score), productive performance (PC2, carcass and head/feet yields), blood (PC3, carbonyls, and TBARS) and meat (PC6, thiols, and TBARS) markers, technological traits (PC4, pH, and color), proximate meat composition (PCA5, moisture, lipids, protein, and ash), fatty acid profile, and nutritional indexes (PC7, IP, and PUFAn-3). Uni- and bivariate analyses showed a strong positive association between kinetic behaviors and blood and meat oxidation and a medium positive association with fatty acid profile and nutritional indexes, whereas a negative association was found between productive performance loads and the technological traits of meat. Generalized linear models showed that all PCs were influenced by genotype. In particular, CY and M resulted as less active genotypes; conversely, RR showed more kinetic activity, whereas RJ, RG, and RC exhibited intermediate levels of activity. Cluster analysis of kinetic behavior and blood or meat oxidative status highlighted 2 groups: nonwalking (NW: CY and M) and walking (W: RC, RG, RR, and RJ) animals. However, in the W group, another was visualized, constituted by genotypes with high kinetic activity resulting in the worst oxidative balance (Walking not trained-genotypes, Wnt: RR and RJ). The present results confirmed that the kinetic behavior of SrG genotypes is negatively correlated with productive performance. Furthermore, a significant association between kinetic behavior and blood (positively correlated) or meat (negatively correlated) oxidative status was noted. Such differences are mainly due to the intrinsic response of the genotypes used (i.e., training-walking capacity).