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Congenital defects are those abnormalities present at birth. During embryogenesis, many anomalies can occur. The primitive gut tube lengthens quickly and rotates, allowing the gastrointestinal tract acquire its final position and orientation. Because the colon of large animals is complex, most changes occur in this segment. Thus, in ruminants, colon atresia is the most frequent malformation, affecting mainly ascending colon, at the level of the spiral loop. There are no previous references about a very atypical colon atresia at the junction of distal loop and transverse colon, such we have described in a 5-day-old calf, after a history of abdominal distention and absence of feces at birth, even with a patent anal opening. Atresia coli was detected at distal position of the typical colon atresia, at the junction of distal loop and transverse colon. In addition, the distal blind end was bent into a U-shape supported by the mesocolon. Besides the anatomical findings of this worthwhile atresia coli we discuss its possible etiology, in which local factors, such as a compromised blood supply during embryogenesis, are more consistent than genetic factors. Finding out the causes of atresia coli would help to reduce its incidence, lessen animal suffering and economic loss.

Abstract: The pathogenesis of pituitary adenomas and many of the genes influencing growth of these tumors are unknown. TGFβ is known to inhibit proliferation of cultured anterior pituitary cells and anterior pituitary tumors, but the signal transduction pathways involved in the inhibition of growth are unclear. We treated the human HP75 pituitary cell line with 10−9 M TGFβ1 for 4, 24, and 96 h and performed global gene expression profiling by Affymetrix GeneChip microarray analysis. Quantitative PCR validation of specific genes involved in the TGFβ1-induced regulation of pituitary cell growth was also done. Of the 15,000 genes queried, there were 37 genes up-regulated and 48 genes down-regulated twofold or more after 4 h of TGFβ1 treatment. There were 121 genes up-regulated and 109 genes down-regulated twofold or more after 24 h of TGFβ1 treatment and 112 genes up-regulated and 43 genes down-regulated twofold or more after 96 h of TGFβ1 treatment. Galectin-3 (Gal-3) protein was decreased by TGFβ1 treatment and several genes which interacted with Gal-3 including RUNX1 and WNT5B were up-regulated after TGFβ1 treatment. SOX4 was also up-regulated by TGFβ1 treatment. SMAD3, which is directly involved in the TGFβ signal transduction pathway, was down-regulated by TGFβ1 treatment. These findings highlight the diverse gene networks and pathways through which TGFβ operates in its effects on pituitary tumor cells.

The accessory olfactory bulb (AOB) is the primary target of the sensory epithelium of the vomeronasal organ (VNO), and thus constitutes a fundamental component of the accessory olfactory system, which is involved in responses to behaviour‐related olfactory stimuli. In this study we investigated the characteristics of the AOB, VNO, vomeronasal nerves (VNNs) and caudal nasal nerve (CdNN) in pigs and sheep, species in which olfaction plays a key behavioural role both in the neonatal period and in adulthood. The patterns of staining of the AOB by the Bandeiraea simplicifoliaand Lycopersicon esculentumlectins were the same in the 2 species, whereas the Ulex europeusand Dolichos bifloruslectins gave different patterns. In both species, lectin staining of the AOB was consistent with that of the VNNs, while the CdNN did not label any of the structures studied. The entire sensory epithelium of the pig was labelled by Ulex europeusand Lycopersicum esculentumlectins, and all 4 lectins used labelled the mucomicrovillar surface of the sensory epithelium in sheep.

This investigation aimed to identify, by the double immunogold procedure, the ultrastructural characteristics of prolactin (PRL) cells in the mink. Such cells showed a marked pleomorphism and had a close topographic relationship with growth hormone cells. A common morphological characteristic of PRL cells in all stages of mink development was the presence of round secretory granules, in contrast to changes in the ultrastructural characteristics of PRL cells with physiological state and photoperiod. Thus PRL cells in prepubertal, pubertal, and sexually inactive adult mink, killed under a short-day photoperiod, showed little development of the organelles but a significantly increased cytoplasmic electron density. In sexually active mink and in lactating females under long-day conditions, PRL cells had a highly developed cytoplasmic organelle structure consisting mainly of rough endoplasmic reticulum. The morphometric study demonstrated that the mean diameter of the secretory granules similarly varied in both sexes. Pubertal mink had PRL cells with smaller secretory granules (female 74.1±0.6 nm, male 80.4±1.7 nm), whereas adult mink killed under a long-day photoperiod presented PRL cells with larger secretory granules (female 194.5±2.2 nm, male 203.3±1.7 nm). The changes in the ultrastructural characteristics of PRL cells during the annual cycle suggest a photoperiodic influence upon these cells. In addition the heterogeneity in ultrastructural characteristics and storage characteristics of PRL in some adult mink may suggest a varying metabolic role for PRL under certain, as yet not fully characterized, conditions.

A simple procedure is described for removing Epon resin from semi-thin 1 μm sections, which permits excellent postembedding immunohistochemical staining (avidin-biotin complex technique). The procedure was developed for the detection of growth hormone and prolactin in bovine adenohypophysis fixed with 2% paraformaldehyde and 0.5% glutaraldehyde in 0.1 msodium cacodylate buffer pH 7.4–7.6. The results indicate that the removal of the epoxy embedding medium prior to the application of the immunohistochemical reagents was essential for the successful localization of the antigenic determinants of the two hormones. The immunocytochemical reactivity was obtained only after treating the sections with a solution of potassium hydroxide in a mixture of absolute methyl alcohol and propylene oxide (Maxwell's solution). An enhanced immunoreactivity was obtained when this treatment was followed by an additional treatment with either 4% hydrogen peroxide or a saturated aqueous solution of sodium metaperiodate. Because of the easy preparation of the Epon removal solution and the good structural preservation without damage to the antigenic determinants, Maxwell's solution is suggested as a good etching agent which can be used in immunohistochemical studies on semi-thin sections with excellent results.

The origin and location of the arteries of the vomeronasal organ (VNO) in the sheep were studied by means of dissection, scanning electron microscopy of corrosion casts, and the cutting-grinding technique after injection with Araldite CY23-HY2967 via one of the carotid arteries. Dissection revealed that the most ventral of the three main branches of the sphenopalatine artery is responsible for the blood supply to the VNO. Scanning electron microscopy of corrosion casts revealed that the arterioles of the vomeronasal organ form a microvascular network. Cross sections of the region of the nasal cavity containing the VNO, obtained by the cutting-grinding technique, showed that the arterioles of the vomeronasal plexus are located medial and ventral to the vomeronasal duct. These results confirm the usefulness of the cutting-grinding technique as a complementary procedure in morphological studies of structures containing hard tissues.

The main goal of this work was to investigate the general distribution of arterial blood around and inside the vomeronasal organ (VNO) of the cat. Macro- and microdissection methods together with light and scanning electron microscopy were used. Heads were injected with an India ink/agar mixture (the VNO subsequently being cut in transverse, sagittal and horizontal sections), with clear latex (the VNO subsequently being cut in transverse sections), or with an epoxy resin to obtain casts for examination by scanning electron microscopy. Dissection and microdissection show that the infraorbital, minor palatine, and descending palatine arteries have a common origin, rostral to the Rete mirabile arteria maxillaris. In transverse series and in the rostral half of the VNO, an arteriole is consistently observed between the vomeronasal duct and the lateral sheet of the vomeronasal cartilage. In this same segment, arterial branches with different orientations (perpendicular, horizontal, or transverse with respect to the main axis of the organ) are observed. Scanning electron microscopy of arterial casts shows that arterial vessels of the mucosa of the nasal septum have a direct relationship with the VNO. Branches of the sphenopalatine artery are the chief route of blood supply to the VNO. The vomeronasal parenchyma has few arterial vessels, and these are usually situated in the same position. Differences observed between the arteries inside and outside the VNO and the dilation of both by isoproterenol support the idea that the VNO is similar to erectile tissue organs and that it may act as a physiological pump. Anat. Rec. 247:129–136 © 1997 Wiley-Liss, Inc.