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Purpose: Integrin αvis a key regulator in the pathophysiology of hepatic fibrosis. In this study, we evaluated the potential utility of an integrin αvβ3positron emission tomography (PET) radiotracer, 18F-labeled cyclic arginine-glycine-aspartic acid penta-peptide ([18F]F-FPP-RGD2), for detecting hepatic integrin αvand function in nonalcoholic steatohepatitis (NASH) model rats using integrin αvsiRNA. Methods: NASH model rats were produced by feeding a choline-deficient, low-methionine, high-fat diet for 8 weeks. PET/computerized tomography imaging and quantification of integrin αvprotein, serum aspartate aminotransferase, and alanine aminotransferase were performed 1 week after single intravenous injection of integrin αvsiRNA. Results: Integrin αvsiRNA (0.1 and 0.5 mg/kg) dose-dependently decreased hepatic integrin αvprotein concentrations in control and NASH model rats. The hepatic mean standard uptake value of [18F]F-FPP-RGD2was decreased dose-dependently by integrin αvsiRNA. The mean standard uptake value was positively correlated with integrin αvprotein levels in control and NASH model rats. Serum aspartate aminotransferase and alanine aminotransferase concentrations were also decreased by siRNA injection and correlated with liver integrin αvprotein expression levels in NASH model rats. Conclusion: This study suggests that [18F]F-FPP-RGD2PET imaging is a promising radiotracer for monitoring hepatic integrin αvprotein levels and hepatic function in NASH pathology.

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.