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Camellia oleiferais an important edible oil woody plant in China. Anthracnose is a serious disease of C. oleifera, causing severe economic losses and posing a huge threat to the C. oleiferaindustry. The fungi Colletotrichum siamenseis one of the main pathogens causing anthracnose of C. oleifera. In this study, antifungal activity of microorganisms isolated from garbage enzyme against C. siamensewere investigated for the first time. 8 strains were isolated and purified from garbage enzyme. By morphological observation, gene sequence analysis, among the strains, 4 isolates were identified as Trichoderma harzianumand 4 isolates were identified as Bacillus subtilis. The result showed that 8 isolates displayed a relatively good potential on inhibiting the mycelial growth in C. siamense, but T. harzianumwas more effective than B. subtilisas a biocontrol agent, with the best efficiency of 76.5%. In the vitro treatments, the combination of T. harzianumand B. subtiliswere the most effective treatments in reducing the incidence and severity of anthracnose in C.oleifera, with the highest control efficiency of 77.56%. It can be concluded that T. harzianumand B. subtilisare promising biocontrol agents to manage anthracnose. These promising results provided valuable information on using garbage enzyme of wastes as a new source of antagonists to control fungi disease.

We developed a variational approach to investigate the ground state energy and the extend of the wavefunction of a neutral donor located near a semiconductor surface in the presence of scanning tunneling microscope (STM) metallic tip. We apply the effective mass approximation and use a variational wavefunction that takes into account the influence of all image charges that arise due to the presence of a metallic tip. The behavior of the ground state energy when the tip approaches the semiconductor surface is investigated.