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Proteinase‐activated receptor‐2 (PAR2) belongs to a novel subfamily of G‐protein‐coupled receptors with seven‐transmembrane domains. PAR2can be activated by serine proteases such as trypsin, mast cell tryptase, and allergic or bacterial proteases. This receptor is expressed by various cells and seems to be crucially involved during inflammation and the immune response. As previously reported, human neutrophils express functional PAR2. However, the precise physiological role of PAR2on human neutrophils and its implication in human diseases remain unclear. We demonstrate that PAR2agonist‐stimulated human neutrophils show significantly enhanced migration in 3‐D collagen lattices. PAR2agonist stimulation also induced down‐regulation of L‐selectin display and up‐regulation of membrane‐activated complex‐1 very late antigen‐4 integrin expression on the neutrophil cell surface. Moreover, PAR2stimulation results in an increased secretion of the cytokines interleukin (IL)‐1β, IL‐8, and IL‐6 by human neutrophils. These data indicate that PAR2plays an important role in human neutrophil activation and may affect key neutrophil functions by regulating cell motility in the extracellular matrix, selectin shedding, and up‐regulation of integrin expression and by stimulating the secretion of inflammatory mediators. Thus, PAR2may represent a potential therapeutic target for the treatment of diseases involving activated neutrophils.