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ABSTRACTRecently, there has been a co-evolution of mammalian libraries and diverse microfluidic approaches for therapeutic antibody hit discovery. Mammalian libraries enable the preservation of full immune repertoires, produce hit candidates in final format and facilitate broad combinatorial bispecific antibody screening, while several available microfluidic methodologies offer opportunities for rapid high-content screens. Here, we report proof-of-concept studies exploring the potential of combining microfluidic technologies with mammalian libraries for antibody discovery. First, antibody secretion, target co-expression and integration of appropriate reporter cell lines enabled the selection of in-transacting agonistic bispecific antibodies. Second, a functional screen for internalization was established and comparison of autocrine versus co-encapsulation setups highlighted the advantages of an autocrine one cell approach. Third, synchronization of antibody-secreting cells prior to microfluidic screens reduced assay variability. Furthermore, a display to secretion switchable system was developed and applied for pre-enrichment of antibody clones with high manufacturability in conjunction with subsequent screening for functional properties. These case studies demonstrate the system’s feasibility and may serve as basis for further development of integrated workflows combining manufacturability sorting and functional screens for the identification of optimal therapeutic antibody candidates.

This paper presents the charging process procedure of Li-ion Battery pack for electric vehicle, which is implemented based on constant current and constant voltage (CC-CV) mode. All the informations regarding battery voltage level, state of charge (SOC) during charging and discharging processes, and battery temperature, is displayed on computer via battery management system (BMS). During the charging process, the BMS monitors the voltage balancing in Li-ion battery pack, as well as the cells voltage in each modules. The voltage difference between the highest voltage cell and the lowest voltage cell is very small, which validates the voltage stability and balance in battery pack during the charging and discharging processes.