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The hemadsorption (HAD) reaction of chick embryo cells infected with mumps virus was studied by means of light and electron microscopy, with special reference to the plasma membrane of the infected cell. The concomitant observation of membrane-free aggregates of viral nucleocapsid in the cytoplasm and attached red blood cells on the surface of the same cell indicated that only infected cells hemadsorbed and that hemagglutinin is confined within the infected cell. The attachment of red blood cells to morphologically intact cell membrane prior to its differentiation into viral envelope suggested that the HAD phenomenon, dependent on the presence of hemagglutinin, was independent of the viral maturation process. The gap of low electron density normally separating the morphologically intact membrane of the tissue culture cell and that of the red blood cell at the binding site was replaced by newly formed surface projections in HAD involving a segment of differentiated plasma membrane.

The fine structure of mumps virus-infected chick embryo fibroblastic cells was examined sequentially after viral inoculation. Intracytoplasmic nucleoprotein strands, similar to those described for parainfluenza viruses, were detectable in small aggregates between 36 and 48 hr. The peripheral strands of this viral component lie beneath and along an antigenically altered bulging portion of the cell membrane. The outermost strands are consistently parallel to the differentiated segment of the plasma membrane, which is invariably associated with surface projections. As has been found with other myxoviruses, mumps virus replicates by budding from the cell surface. The virus particle, roughly spherical in shape, has a size ranging from 1,000 to 8,000 A. Filamentous forms are rarely observed in the present culture system. Ferritin-conjugated antibody specifically labels the cytoplasmic nucleoprotein, the modified cell membrane, and the virus particle. Intranuclear inclusions of low electron density and morphologically different from those described in measles virus-infected HeLa and amnion cells were observed in the nucleus of several infected cells. Immuno-electron microscopic observations suggest that the nucleoprotein synthesis rate exceeds that of cell membrane differentiation into viral envelope. This difference results in the accumulation of viral nucleoprotein in large intracytoplasmic masses which can be demonstrated by electron microscopy.

Duc-Nguyen, Huu(National Cancer Institute, Bethesda, Md.), Edith N. Rosenblum, and Robert F. Zeigel. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J. Bacteriol. 92:1133–1140. 1966.—The propagation of a murine leukemia virus (Rauscher) in a kidney cell line, derived from a rat with lymphoid leukemia, was studied. A complement-fixing (CF) antigen reacting with Rauscher immune sera was detected at various passage levels, which correlated with the visualization by use of electron microscopy of viral buds and viral particles in different stages of maturation in all passages. Five-month-old monolayers continued to shed virus and to yield high CF antigen titers. The cell-free supernatant fluid from cultures of the 14th passage was shown to be infectious for a normal rat kidney cell line, as evidenced by the appearance of the CF antigen in this line. Interferon production was not demonstrated in infected cultures. The overall data indicated that rat kidney cells could be used to propagate Rauscher virus in a carrier state.

Duc-Nguyen, Huu(The Children's Hospital of Philadelphia, Philadelphia, Pa.), and Werner Henle. Replication of mumps virus in human leukocyte cultures. J. Bacteriol. 92:258–265. 1966.—Human peripheral leukocyte cultures maintained in the presence of phytohemagglutinin (PHA) were found to support to some extent the replication of mumps virus. When such cultures were exposed, within 24 hr after their initiation, to a high input multiplicity of virus, successful infection, as determined by immunofluorescence and plaque assays, did not become evident before the 3rd or 4th day. On exposure of cultures 4 to 5 days old, viral replication was detectable within 2 days. In both instances, peak immunofluorescence and virus titers were reached when the cultures were 7 to 9 days old and composed mainly of blast forms. With decreasing input multiplicities of infection, cells containing viral antigen and production of infectious viral progeny became detectable with increasing delay. No significant viral replication was noted in surviving cells maintained in the absence of PHA. These results indicate that mainly, if not solely, the PHA-stimulated cells of the lymphocytic series support viral multiplication. The extent of the infectious process was limited, however, because the life span of the cultures was not significantly shortened, the yields of infectious virus per immunofluorescent cell were at all times low, and most infected cells contained only a few well-delineated small masses of antigen, suggestive of an abortive infection. Only fresh cultures were capable of synthesizing interferon on stimulation by mumps, Newcastle disease, or Sendai viruses. When the cultures were set up in the presence of PHA, this capacity was lost within 24 hr. PHA per sefailed to induce detectable production of an interferon under the conditions used. The implications of these findings are discussed.

Magnetisation and susceptibility measurements are reported for the pseudobinary intermetallic compounds TbxY1−xCu2(x= 0.1, 0.3, 0.5, 0.7, 0.9 and1). Metamagnetic behaviour was observed at low temperatures in this system, except for Tb0.1Y0.9Cu2. The critical magnetic field and the Néel temperature smoothly decrease with increasing Y content. The temperature dependence of the magnetisation of TbCu2was studied. The interaction parameters between Tb3+ions were determined for the compounds investigated.

Magnetic field sensors based on the planar Hall effect using single layer Ni80Fe20thin films have been designed, fabricated and characterized. By optimizing the sensor's dimension, such as the thickness and the length to width ratio, experimental results have shown that the sensor's sensitivity could be increased up to three times thanks to the enhancement of the shape magnetic anisotropy. The highest sensitivity of 100 ?V Oe?1corresponding to the relative resistance change up to 20 m?/Oe was achieved at a supplied DC current of 5 mA for the cross-shaped geometry of the sensor with 5 nm in the thickness and a length/width ratio of 10. Simple structure, low fabrication cost, and low power consumption make this sensor very promising for low field magnetic field sensing such as geomagnetic field detections.

BackgroundThe relationship between temperature and mortality has been found to be U-, V-, or J-shaped in developed temperate countries; however, in developing tropical/subtropical cities, it remains unclear.ObjectivesOur goal was to investigate the relationship between temperature and mortality in Hue, a subtropical city in Viet Nam.DesignWe collected daily mortality data from the Vietnamese A6 mortality reporting system for 6,214 deceased persons between 2009 and 2013. A distributed lag non-linear model was used to examine the temperature effects on all-cause and cause-specific mortality by assuming negative binomial distribution for count data. We developed an objective-oriented model selection with four steps following the Akaike information criterion (AIC) rule (i.e. a smaller AIC value indicates a better model).ResultsHigh temperature-related mortality was more strongly associated with short lags, whereas low temperature-related mortality was more strongly associated with long lags. The low temperatures increased risk in all-category mortality compared to high temperatures. We observed elevated temperature-mortality risk in vulnerable groups: elderly people (high temperature effect, relative risk [RR]=1.42, 95% confidence interval [CI]=1.11–1.83; low temperature effect, RR=2.0, 95% CI=1.13–3.52), females (low temperature effect, RR=2.19, 95% CI=1.14–4.21), people with respiratory disease (high temperature effect, RR=2.45, 95% CI=0.91–6.63), and those with cardiovascular disease (high temperature effect, RR=1.6, 95% CI=1.15–2.22; low temperature effect, RR=1.99, 95% CI=0.92–4.28).ConclusionsIn Hue, the temperature significantly increased the risk of mortality, especially in vulnerable groups (i.e. elderly, female, people with respiratory and cardiovascular diseases). These findings may provide a foundation for developing adequate policies to address the effects of temperature on health in Hue City.

Recently, the electric field induced manipulation of magnetic properties has become one of the most interesting research topics because of its promising applications in magnetoelectronics, such as future logic elements and electric-magnetic memories. In this paper, the NiFe/CoFe ferromagnetic nanostructured thin films were directly deposited on the ferroelectric PZT substrate, which was polarized parallel to the thickness or along the surface. In these samples, the stress originating from the piezoelectric layer is transferred into the magnetostrictive layer, which induces an elastic strain and results in a change in magnetization, thanks to the converse magnetoelectric effect. By analyzing the magnetization of the nanocomposite under a fixed magnetic field up to 5×103?Oe at different voltages applied to the ferromagnetic substrate, we can investigate the voltage-controllable magnetization in this composite. The obtained results show that the magnetization of the NiFe/CoFe film changes around 6% at the low voltage of 60?V and up to two times larger at 800?V in an external magnetic field of 50?Oe. The use of voltage also allows the reversible adjustment of the magnetization orientation in ferromagnetic layers.

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