1. Cloning and expression analysis of HXK1gene from Jatropha curcasL.
- Author
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Xin, Hu, Tang, Lizhou, Liu, Chao, Gao, Yong, Dai, Dongqin, Chen, Huanhuan, Deng, Yan, Tian, Bin, and Wang, Haibo
- Abstract
HXK (Hexokinase) gene family and the role of JcHXK1in Jatropha curcasL. were explored. Totally 4 HXKgenes JcHXK1, JcHXK2, JcHXK3and JcHKL1were identified and complete ORF of JcHXK1was cloned. Functional domain, phylogenetic evolution and low-temperature expression characteristics were analyzed. Results showed that full-length JcHXK1cDNA was 1497 bp, encoding 498 amino acids with molecular weight of 53.81 ​kDa and pI of 5.03. Further phylogenetic evolutionary analysis demonstrated HXK1 protein was clustered into 6 small branches and 2 large branches. Sequence alignment showed that HXK1 contained several conserved glycine residues and hydrophobic channels. Prokaryotic expression vector of JcHXK1was constructed and 12% SDS-PAGE detection showed that it was highly expressed in E. coli. These research was expected to lay a foundation for further gene functional verification and cold signal transduction mechanism for HXK1in Jatropha curcasL.
- Published
- 2020
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