1. Mutations within gene XNR_2147for TetR-like protein enhance lincomycin resistance and endogenous specialized metabolism of Streptomyces albusJ1074
- Author
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Tseduliak, Vasylyna-Marta, Dolia, Borys, Ostash, Iryna, Lopatniuk, Maria, Busche, Tobias, Ochi, Kozo, Kalinowski, Jörn, Luzhetskyy, Andriy, Fedorenko, Victor, and Ostash, Bohdan
- Abstract
Streptomyces albusJ1074 is one of the most popular heterologous expression platforms among streptomycetes. Identification of new genes and mutations that influence specialized metabolism in this species is therefore of great applied interest. Here, we describe S. albusKO-1304 that was isolated as a spontaneous lincomycin-resistant variant of double rpsLR94GrsmGR15SG40Emutant KO-1295. Besides altered antibiotic resistance profile, KO-1304 exhibited increased antibiotic activity as compared to its parental strains. KO-1304 genome sequencing revealed mutations within gene XNR_2147encoding putative TetR-like protein. Gene XNR_2146for efflux protein is the most likely target of repressing action of Xnr_2147. Our data agree with the scenario where lincomycin resistance phenotype of KO-1304 arose from inability of mutated Xnr_2147 protein to repress XNR_2146. Introduction of additional copy of XNR_2146into wild type strain increased antibiotic activity of the latter, attesting to the practical value of transporter genes for strain improvement.
- Published
- 2023
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