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Sarcoid tumors were described by means of histopathological and molecular procedures in a 5-year-old donkey. Histopathological examination showed epithelial changes including hyperkeratosis, epithelial hyperplasia, koilocytosis, and rete peg formation. Neoplastic fibroblastic cells were plumb, large spindle to stellate and embedded in dense collagenous tissue. Results of Polymerase Chain Reaction and DNA sequence analysis showed that the etiological agent belonged to Bovine Papilloma Virus-II species in the delta papilloma virus genus. This case study represents the first report demonstrating the presence of Bovine Papilloma Virus-II in donkey sarcoid.

Concurrent occurence of bovine papular stomatitis, rotavirus infection and cryptosporidiosis was diagnosed postmortem in a 7-days-old calf from a farm containing 65 calves of different ages. Multifocal papular stomatitis and rumenitis were present on necropsy. While polymerase chain reaction analysis revealed rotavirus and papular stomatitis virus infections; bovine viral diarrhea, foot and mouth disease, bovine papilloma virus and coronavirus could not be detected. Overall; concurrent co-infection with bovine papular stomatitis virus, rotavirus and cryptosporidium spp. was reported for the first time.