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Adult patients with sickle cell disease (SCD) are highly susceptible to stem cell transplant complications, including drug toxicity, graft versus host disease (GVHD), and graft rejection due to SCD-related tissue damage, endothelial activation, and inflammation. The scarcity of compatible stem cells for transplantation further limits treatment options, with only 43 cases of adult allogeneic peripheral blood stem cell transplantation (allo-PSCT) from human leukocyte antigen (HLA)-identical sibling donors reported in the international registry for the period 1986–2013. Herein we report remarkable outcomes in a cohort of adult SCD patients who underwent allo-PSCT using a fludarabine (Flu), busulfan (Bu), and anti-T-cell lymphocyte globulin (ATG)-based conditioning regimen in combination with very low dose total body irradiation (TBI), followed by post-transplant cyclophosphamide (Cy) and sirolimus as GVHD prophylaxis. We performed a single-center, retrospective study consisting of 20 consecutive patients (mean age 33.4 years) who underwent allo-PSCT from HLA-matched related donors with a conditioning regimen of Flu 150/Bu 3.2/Cy 29/ATG 30 (Fresenius)/TBI 200 between September 2013 and September 2017. Data were validated by an independent data audit group of the affiliated JACIE-accredited transplantation center. All patients experienced a sustained donor cell engraftment. Full donor chimerism (total cell) occurred within 180 days in all patients. Mean duration of follow-up was 13.8 months (range: 0.3–50 months), with 12 (60%) patients completing 12 months. No non-relapse mortality or graft rejection occurred. Successful treatment was achieved without the presence of graft loss, grade III–IV acute GVHD, extensive chronic GVHD, or other major complications. Allo-PSCT in combination with Flu 150/Bu 3.2/Cy 29/ATG 30(Fresenius)/TBI 200- Cy/Sirolimus therapy yielded encouraging outcomes with no mortality and low incidence of GVHD. Further controlled studies will be necessary to compare transplant protocols and long-term outcomes.

Hydroxystearic acid (HSA) molecules at the air−water interface present an interesting bicompetitive adsorption between primary and secondary hydrophilic groups on either end of an alkyl chain, which, depending on the position of the second hydrophilic group, may lead to a sharp transition from an expanded phase to a crystalline condensed morphology as surface pressure is increased. Here we report a set of measurements on a series of hydroxystearic acids in which the position of the secondary competing hydrophilic group position is varied along the whole extent of the alkyl chain from position 2 (i.e., close to the primary hydrophilic group) to positions 7, 9 and 12, the latter being the compounds mostly studied in the literature. We show here direct microscopic evidence, obtained by synchrotron radiation reflectometry and grazing incidence diffraction, that the position of the secondary hydrophilic group not only strongly influences the phase diagram as determined by compression isotherms and ellipsometry but also induces different crystallization patterns in the 2D system of the Langmuir monolayer. In particular, we report for the first time the existence of a turning point in the effects of the hydroxyl position on the monolayers structure at 7-HSA.

A sensitive, specific, accurate and reproducible gas chromatography/mass spectrometry method was developed for the assay of 9‐ and 10‐hydroxystearic acids in samples obtained as cell extracts. The preparation of the samples required specific procedures to allow the analysis of both the free and the conjugated hydroxy acids as the corresponding methyl esters. The quantification used propyl‐paraben as the internal standard and monitoring of a specific fragment of each isomeric hydroxy acid methyl ester, and allowed quantification of the conjugate and the free fractions of both 9‐ and 10‐hydroxystearic acids. This method is suitable for identification and quantification (LOQ 1.8 and 4.4 ng, respectively) of these important metabolites of lipid peroxidation. In particular the development of an assay for the free 9‐hydroxystearic acid methyl ester makes the method a reliable analytical tool for investigations of the role of this metabolite in the mechanisms of tumour cell proliferation. Copyright © 2002 John Wiley & Sons, Ltd.

trans-4-Hydroxy-2-nonenal (HNE) is an end-product of lipid peroxidation in biological systems which produces a variety of powerful biological effects. A method based on electrospray mass spectrometry was developed for the determination of 4-HNE at cellular levels. Quantification was carried out by using HNE-d11 as internal standard; the mass chromatograms were acquired in the single ion monitoring mode (SIM) on the [M + H]⁺ monoisotopic species for HNE and HNE-d11. With this approach a higher precision and lower detection limit and biological sample size than those typical of the methods so far employed are achieved. Furthermore the determination of the analyte from the cell extract is directly performed without the need of any HNE derivatization. As a first application the method was used to identify and quantify HNE in human T cell leukemia extracts. Copyright © 1999 John Wiley & Sons, Ltd.

trans‐4‐Hydroxy‐2‐nonenal (HNE) is an end‐product of lipid peroxidation in biological systems which produces a variety of powerful biological effects. A method based on electrospray mass spectrometry was developed for the determination of 4‐HNE at cellular levels. Quantification was carried out by using HNE‐d11as internal standard; the mass chromatograms were acquired in the single ion monitoring mode (SIM) on the [M + H]+monoisotopic species for HNE and HNE‐d11. With this approach a higher precision and lower detection limit and biological sample size than those typical of the methods so far employed are achieved. Furthermore the determination of the analyte from the cell extract is directly performed without the need of any HNE derivatization. As a first application the method was used to identify and quantify HNE in human T cell leukemia extracts. Copyright © 1999 John Wiley & Sons, Ltd.

No Abstract.

The reactions of methylcopper‐ and dimethylcuprate‐boron trifluoride reagents with aldimines such as (I), derived from (S)‐1‐phenylethylamine, yield with good diastereoselectivity the (1S,1'S)‐amines (IIIa) and (IIIb) from aromatic imines.

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.