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We have used natural abundance13C magnetic resonance spectroscopy (MRS) to measure glycogen content of muscle and liver before and after heavy exercise, and after consumption of different carbohydrate-based drinks. After an overnight fast, five healthy men (mean±SEM age 23±1 years) exercised to exhaustion at 75% of VO2max on two occasions (mean work rate 165±8 W for 78±14 min) and then, in a single blind random order, consumed either of two drinks containing the same carbohydrate load (177 g). Spectra were recorded over Vastus Lateralis muscle and the liver before and after exercise, and hourly for 5 h after the carbohydrate load. In muscle, glycogen content after exercise was 37% and 31% of basal (preexercise) concentration before consuming the drinks. After carbohydrate loading, glycogen concentration had increased significantly (p<0.05) to 70% and 64% of basal concentration respectively after 5 h. Hepatic glycogen concentration did not change significantly throughout. The study demonstrates the feasibility of sequential MRS measurement of muscle and liver glycogen before and after exercise and after carbohydrate loading.

Zusammenfassung.: 37 Patienten mit chronischem Pilonidalsinus wurden in dem Zeitraum von Mai 1993–Mai 1997 nach Totalexcision und Revision der Steibeinspitze mit einer Lappenplastik nach Dufourmentel versorgt. Nachuntersucht wurden 32 Patienten. Bei 3 Patienten entwickelte sich postoperativ ein punktionswrdiges Wundserom, bei 2 Patienten kam es zu einer Sekundrheilung. Die durchschnittliche Krankenhausverweildauer lag bei 6,3 Tagen, die durchschnittliche Arbeitsunfhigkeit bei 2,5 Wochen. Die Methode ist einfach durchfhrbar, Rezidive sind in bereinstimmung mit der Literatur nicht bekannt.

[4-14C]Testosterone was incubated with human male scalp skin and the metabolites identified as 5α-androstane-3α, 17β-diol, androsterone, epiandrosterone, dihydrotestosterone, androstenedione and androstanedione. A metabolite not previously reported from incubations of human skin with androgens was isolated and is probably a steroid ester.One bald and one hairy scalp biopsy specimen from each of five subjects were incubated with [4-14C]testosterone for 1 h and the radioactivity of each metabolite was measured after separation by thin-layer chromatography. Both the uptake and metabolism of testosterone were greater in bald skin than in hairy skin. Little difference in the percentage of each metabolite in the total metabolite pool was found between bald and hairy skin from the same subject and skin from different subjects. This demonstrated that male pattern baldness was not accompanied by gross changes in testosterone metabolism. The main finding was that bald skin contained increased amounts of 5α-reduced metabolites.