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This article discusses the discovery of a conserved hub protein, WthA, in Acinetobacter baumannii that plays a crucial role in coordinating peptidoglycan turnover and activating cell division amidases. The researchers found that WthA is essential in cells lacking lipooligosaccharide (LOS), a variant of lipopolysaccharide (LPS), and that mutants lacking WthA exhibited severe cell division defects. The study also identified homologs of WthA in other bacteria, suggesting that this protein family is involved in regulating peptidoglycan turnover across different species. However, the researchers did not find evidence that the homologous protein in Pseudomonas, LbcA, functions as an amidase activator. [Extracted from the article]

A patent application has been filed for the use of certain agents in preventing the transition from acute to chronic pain. The method involves administering these agents to patients between 1 and 7 days after a traumatic pain event, such as physical injury, invasive surgery, or acute illness. The agents mentioned include NAAA inhibitors, FAAH inhibitors, PPARa agonists, PEA, acetyl-L-carnitine, a-lipoic acid, and olesoxime. This method could potentially help prevent chronic pain in various patient populations, including those with cancer or diabetes. [Extracted from the article]

A recent preprint abstract from biorxiv.org discusses the discovery of a new family of chimeric effectors in the type VI secretion system (T6SS). The effector, TseP, from Aeromonas dhakensis, was found to have dual amidase-lysozyme activities. The research demonstrates that these effectors target peptidoglycan, offering a potential strategy to combat antimicrobial resistance. The study also highlights the structural role of the N-domain of TseP and its interaction with a carrier protein. However, it is important to note that this preprint has not yet undergone peer review. [Extracted from the article]

Researchers at the Indian Institute for Technology in Delhi, India have reported new data on gene libraries. They prepared metagenomic fosmid libraries from soil and water samples collected from various locations and used them to screen and isolate D-amidase producers. The researchers found that the maximum number of libraries were obtained from soil rich in fishery waste, while the minimum number were obtained from tap water. They isolated a novel enzyme that showed catalytic promiscuity with various amides and had acyl transferase activity. The research has been peer-reviewed and published in Applied Biochemistry and Microbiology. [Extracted from the article]

A recent study investigated the potential use of novel endolysins from Streptomyces phages as antimicrobial agents. Endolysins are phage-encoded proteins that can degrade bacterial peptidoglycan and disrupt bacterial growth. The study identified three specific endolysins, LL35lys, Nb26lys, and Tb42lys, which exhibited PG-degrading activity and potential antimicrobial activity against bacteria associated with antibiotic resistance. LL35lys was effective against Gram-positive ESKAPE strains, while Nb26lys and Tb42lys showed activity against Gram-negative bacteria. These findings suggest that endolysins from Streptomyces phages have the potential to be used as antimicrobial agents against ESKAPE bacteria. However, it is important to note that this study has not yet undergone peer review. [Extracted from the article]

A recent report from Technical University Munich (TU Munich) provides new insights into Staphylococcus, a type of bacteria. The study focused on the autolysis process, which is the breakdown of bacterial cells by enzymes. Researchers analyzed the behavior of two strains of Staphylococcus carnosus and found that a specific enzyme called AtlC plays a key role in autolysis. The study also revealed that AtlC is degraded into smaller fragments over time, which remain active in breaking down the cell wall. This research contributes to a better understanding of Staphylococcus and its autolytic behavior. [Extracted from the article]

Researchers at Nanjing Normal University in China have detailed their findings on the rapid degradation of hazardous amides by immobilized engineered Pseudomonas putida KT2440. The amides 4-trifluoromethylnicotinamide, acrylamide, and benzamide are commonly used in agriculture and industry, posing risks to the environment and animals. The study developed a novel and effective immobilized biocatalyst for the remediation of wastewater containing these hazardous amides. The research was supported by the National Natural Science Foundation of China and other funding sources. [Extracted from the article]

A recent preprint abstract discusses the heterodimerization of endolysin isoforms during bacterial infection by Staphylococcal phage {varphi}2638A. The study focuses on the production and function of short variant (SV) isoforms alongside the full-length (FL) endolysin. Through X-ray crystallography structures and AlphaFold-generated models, the researchers were able to determine the individual functions of different domains of the endolysin. They found that the production of both isoforms provides an advantage during phage infection, as phages producing only one isoform had impaired lytic activity. The researchers propose that SV isoform production ensures optimal peptidoglycan degradation prior to cell wall lysis and progeny phage release. [Extracted from the article]

Researchers from the University of Lisbon have published new data on staph infections, specifically focusing on the impact of cellular and enzymatic determinants on the exolytic action of an anti-staphylococcal enzybiotic. The study explores the use of bacteriophage endolysins as potential weapons against antibiotic-resistant bacteria. The researchers found that the bacterial membrane proton motive force (PMF) and certain cell wall glycopolymers of Gram-positive bacteria play a role in tolerance to endolysins. The study concludes that the PMF and wall teichoic acids (WTAs) interfere differently with the functional domains of endolysins, affecting both binding and catalytic efficiencies. [Extracted from the article]

Researchers from Zagazig University in Egypt have made new findings on Aspergillus fumigatus, a fungus that produces acrylamide, a potentially toxic and carcinogenic compound found in overheated foods. The researchers purified an enzyme called amidase from Aspergillus fumigatus, which showed significant activity in degrading acrylamide in various food products such as meat, cookies, potato chips, and bread. The enzyme degraded about 95% of acrylamide to acrylic acid, suggesting its potential use in reducing the levels of acrylamide in food products. Further research is ongoing to assess the enzyme's affinity for selective hydrolysis of acrylamide without affecting other beneficial compounds. [Extracted from the article]

A recent report from Matsuyama University in Ehime, Japan, provides new information on genomics and genetics. The research focuses on Clostridioides difficile, a pathogen that causes pseudomembranous colitis, and the need for novel antimicrobial agents for treatment. The study identifies a gene encoding an endolysin, Ecd18980, which exhibits lytic activity against C. difficile. The researchers also characterized the biochemical properties of Ecd18980, including its optimal pH, salt concentration, and thermal stability. This research has been peer-reviewed and may be of interest to those studying drugs and therapies, enzymes and coenzymes, and pharmaceuticals. [Extracted from the article]

Keywords: Valhalla; State:New York; United States; North and Central America; Amidase; Amidohydrolases; Amino Acids; Basic Amino Acids; Cancer; Diamino Amino Acids; Enzymes and Coenzymes; Glutaminase; Glutamine; Health and Medicine; Neutral Amino Acids; Nitrogenous Group Transferases; Oncology; Transaminases EN Valhalla State:New York United States North and Central America Amidase Amidohydrolases Amino Acids Basic Amino Acids Cancer Diamino Amino Acids Enzymes and Coenzymes Glutaminase Glutamine Health and Medicine Neutral Amino Acids Nitrogenous Group Transferases Oncology Transaminases 1135 1135 1 10/09/23 20231010 NES 231010 2023 OCT 10 (NewsRx) -- By a News Reporter-Staff News Editor at Cancer Weekly -- A new study on Cancer is now available. However, mammalian tissues/cancers possess a rarely mentioned, alternative pathway (the glutaminase II pathway): L-glutamine is transaminated to alpha-ketoglutaramate (KGM), followed by omega-amidase (omega A)-catalyzed hydrolysis of KGM to alpha-ketoglutarate. This pathway contrasts with the much less studied GT omega A (glutamine transaminase-omega-amidase or glutaminase II) pathway (pathway 2): [L-glutamine reversible arrow alpha-ketoglutaramate (KGM) reversible arrow alpha-ketoglutarate -> TCA cycle]. [Extracted from the article]

Amidase, Amidohydrolases, Amino Acids, Basic Amino Acids, Cancer, Diamino Amino Acids, Enzymes and Coenzymes, Glutaminase, Glutamine, Health and Medicine, Neutral Amino Acids, Nitrogenous Group Transferases, Oncology, Transaminases Keywords: Amidase; Amidohydrolases; Amino Acids; Basic Amino Acids; Cancer; Diamino Amino Acids; Enzymes and Coenzymes; Glutaminase; Glutamine; Health and Medicine; Neutral Amino Acids; Nitrogenous Group Transferases; Oncology; Transaminases EN Amidase Amidohydrolases Amino Acids Basic Amino Acids Cancer Diamino Amino Acids Enzymes and Coenzymes Glutaminase Glutamine Health and Medicine Neutral Amino Acids Nitrogenous Group Transferases Oncology Transaminases 1366 1366 1 09/11/23 20230912 NES 230912 2023 SEP 12 (NewsRx) -- By a News Reporter-Staff News Editor at Cancer Weekly -- Fresh data on cancer are presented in a new report. However, mammalian tissues/cancers possess a rarely mentioned, alternative pathway (the glutaminase II pathway): l-glutamine is transaminated to a-ketoglutaramate (KGM), followed by o-amidase (oA)-catalyzed hydrolysis of KGM to a-ketoglutarate. [Extracted from the article]

Keywords for this news article include: Hangzhou, People's Republic of China, Asia, Amidase, Amides, Antibiotics, Antiinfectives, Bacterial Infections and Mycoses, Beta-Lactam Antibiotics, Drugs and Therapies, Enzymes and Coenzymes, Health and Medicine, Natural Penicillins, Organic Chemicals, Penicillin G Therapy, Penicillin Therapy, Penicillins, Pharmaceuticals, Hangzhou Medical College. Keywords: Hangzhou; People's Republic of China; Asia; Amidase; Amides; Antibiotics; Antiinfectives; Bacterial Infections and Mycoses; Beta-Lactam Antibiotics; Drugs and Therapies; Enzymes and Coenzymes; Health and Medicine; Natural Penicillins; Organic Chemicals; Penicillin G Therapy; Penicillin Therapy; Penicillins; Pharmaceuticals EN Hangzhou People's Republic of China Asia Amidase Amides Antibiotics Antiinfectives Bacterial Infections and Mycoses Beta-Lactam Antibiotics Drugs and Therapies Enzymes and Coenzymes Health and Medicine Natural Penicillins Organic Chemicals Penicillin G Therapy Penicillin Therapy Penicillins Pharmaceuticals 1588 1588 1 06/12/23 20230616 NES 230616 2023 JUN 16 (NewsRx) -- By a News Reporter-Staff News Editor at Drug Week -- Investigators publish new report on Bacterial Infections and Mycoses. [Extracted from the article]

In this work, a genome-based screening approach of the genome of E. faecium allowed the identification of a putative PDE gene with predictive amidase activity (EfAmi1; EC 3.5.1.28) in a prophage-integrated sequence. Keywords: Amidase; Bacteriophages; Enterococcaceae; Enterococcus; Enterococcus faecium; Enzymes and Coenzymes; Gram-Positive Bacteria; Lactobacillales; Prophages EN Amidase Bacteriophages Enterococcaceae Enterococcus Enterococcus faecium Enzymes and Coenzymes Gram-Positive Bacteria Lactobacillales Prophages 3232 3232 1 05/29/23 20230602 NES 230602 2023 JUN 2 (NewsRx) -- By a News Reporter-Staff News Editor at Health & Medicine Week -- Researchers detail new data in Enterococcus faecium. Amidase, Bacteriophages, Enterococcaceae, Enterococcus, Enterococcus faecium, Enzymes and Coenzymes, Gram-Positive Bacteria, Lactobacillales, Prophages. [Extracted from the article]

Keywords: Shanghai; People's Republic of China; Asia; Metabolism Research; Health and Medicine; Amidase; Amino Acids; Ammonia; Enzymes and Coenzymes; Nitrogen Compounds; Organic Chemicals; Peptides; Proteins; Urea EN Shanghai People's Republic of China Asia Metabolism Research Health and Medicine Amidase Amino Acids Ammonia Enzymes and Coenzymes Nitrogen Compounds Organic Chemicals Peptides Proteins Urea 1710 1710 1 05/15/23 20230519 NES 230519 2023 MAY 19 (NewsRx) -- By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators publish new report on Health and Medicine - Metabolism Research. SIRT4 knockout in cultured cells caused higher OTCCP-K307 levels, activated OTC, elevated urea cycle intermediates and urea production via amino acid catabolism. [Extracted from the article]

Keywords: Lund; Sweden; Europe; Life Science; Amidase; Bacterial Polysaccharides; Bacteriophages; Drugs and Therapies; Enzymes and Coenzymes; Glycopeptides; Mucoproteins; Peptides; Peptidoglycan; Prophages; Proteins EN Lund Sweden Europe Life Science Amidase Bacterial Polysaccharides Bacteriophages Drugs and Therapies Enzymes and Coenzymes Glycopeptides Mucoproteins Peptides Peptidoglycan Prophages Proteins 1243 1243 1 04/03/23 20230407 NES 230407 2023 APR 7 (NewsRx) -- By a News Reporter-Staff News Editor at Drug Week -- Research findings on Life Science are discussed in a new report. Lund, Sweden, Europe, Life Science, Amidase, Bacterial Polysaccharides, Bacteriophages, Drugs and Therapies, Enzymes and Coenzymes, Glycopeptides, Mucoproteins, Peptides, Peptidoglycan, Proteins, Prophages. [Extracted from the article]