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Octanol–water partition coefficients (log Po/w) of fifteen previously synthesized 17β-carboxamide glucocorticoid derivatives and prednisolone were determined using shake-flask method. The retention behavior of selected compounds was tested in five reversed-phased thin-layer chromatography (RP-TLC) systems, consisting of water and organic solvent (acetonitrile, acetone, ethanol 96%, methanol, or tetrahydrofuran), and chromatography parameters (RM0, S, and C0) were calculated. Simple linear regression (SLR) analysis was performed, and the correlation between log Po/w and chromatography parameters was tested. The most appropriate RP-TLC system for log Po/w prediction was that consisting of water and ethanol 96% as the mobile phase. Statisitical evaluation of the quality of models created using this system proved their reliability for log Po/w prediction of new 17β-carboxamide glucocorticoid derivatives, and the model with the most favorable statistical parameters was underlined.

This paper describes a simple, rapid, and reliable HPTLC procedure for the determination of cyproterone acetate and ethinylestradiol in tablets. Identification and determination were performed on 10 cm × 20 cm LiChrospher silica gel 60 F254HPTLC plates with cyclohexane—ethyl acetate, 6 + 4 (v/v), as mobile phase. Calibration plots were established showing the dependence of response (peak area) on amount chromatographed. The ranges validated were 250–4000 ng (r= 0.9923) and 100–500 ng (r= 0.9996) for cyproterone acetate and ethinylestradiol, respectively. The suitability of this HPTLC method for quantitative determination of the compounds was proved by validation of precision and accuracy. The method was used for determination of the compounds in commercial pharmaceutical dosage forms (Androcur 10 mg and Diane 35 tablets). The method is simple, reproducible, and accurate and can be used as a more effective option than other chromatographic techniques in routine quality control.