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[EN] The susceptibility of the grapevine rootstocks most commonly used in Spain to black-foot (Ilyonectria liriodendri and Dactylonectria macrodidyma-complex) and petri disease (Cadophora luteo-olivacea, Phaeomoniella chlamydospora and five species of Phaeoacremonium) pathogens was evaluated. Rooted cuttings of rootstocks 110R, 1103P, 140Ru, 161-49C, 196-17C, Fercal and SO4 were inoculated with black-foot pathogens by dipping their roots in conidial suspensions (5×105 conidia mL-1), planted in pots containing sterilized peat moss and placed in a greenhouse. After four months of incubation, root disease severity index and dry weights of shoots and roots were recorded for each plant. Regarding petri disease pathogens, one-year-old grapevine cuttings of five rootstocks (41B, 140Ru, 161-49C, 1103P and 110R) were vacuum-inoculated with spore suspensions (106 conidia mL-1) of the fungal species and planted in two fields. After four months, the proportion of vines that sprouted in spring was visually determined. At the end of the growing season, dormant plants were uprooted, washed, and assessed for undried shoot weight. Then, the stem of each grapevine cutting was transversally split at 10 cm from the base of the plant to estimate the percentage of vascular tissue discoloured on a scale of 0 to 4. All rootstocks inoculated with Ilyonectria and Dactylonectria species were affected by the disease in some degree, the rootstock 110R being the most susceptible to black-foot. Petri disease pathogens caused a significant reduction of sprouting and shoot weight, as well as a significant increase of disease severity percentage in all grapevine rootstocks with the exception of 161-49C. Both 110R and 140Ru were the most susceptible. Grapevine rootstocks showed different levels of disease resistance, being the rootstock 110R the most susceptible to both diseases and the 161-49C rootstock the most tolerant to petri disease infection.

The susceptibility of the grapevine rootstocks most commonly used in Spain to black-foot (Ilyonectria liriodendri and Dactylonectria macrodidyma-complex) and petri disease (Cadophora luteo-olivacea, Phaeomoniella chlamydospora and five species of Phaeoacremonium) pathogens was evaluated. Rooted cuttings of rootstocks 110R, 1103P, 140Ru, 161-49C, 196-17C, Fercal and SO4 were inoculated with black-foot pathogens by dipping their roots in conidial suspensions (5×105 conidia mL-1), planted in pots containing sterilized peat moss and placed in a greenhouse. After four months of incubation, root disease severity index and dry weights of shoots and roots were recorded for each plant. Regarding petri disease pathogens, one-year-old grapevine cuttings of five rootstocks (41B, 140Ru, 161-49C, 1103P and 110R) were vacuum-inoculated with spore suspensions (106 conidia mL-1) of the fungal species and planted in two fields. After four months, the proportion of vines that sprouted in spring was visually determined. At the end of the growing season, dormant plants were uprooted, washed, and assessed for undried shoot weight. Then, the stem of each grapevine cutting was transversally split at 10 cm from the base of the plant to estimate the percentage of vascular tissue discoloured on a scale of 0 to 4. All rootstocks inoculated with Ilyonectria and Dactylonectria species were affected by the disease in some degree, the rootstock 110R being the most susceptible to black-foot. Petri disease pathogens caused a significant reduction of sprouting and shoot weight, as well as a significant increase of disease severity percentage in all grapevine rootstocks with the exception of 161-49C. Both 110R and 140Ru were the most susceptible. Grapevine rootstocks showed different levels of disease resistance, being the rootstock 110R the most susceptible to both diseases and the 161-49C rootstock the most tolerant to petri disease infection.

A questionnaire covering all aspects of grapevine propagation including cultural and sanitation practices in mother blocks and harvest and transport of cuttings from mother blocks to nurseries, nursery operations and field nursery management, was mailed to all Management Committee members of the European COST Action FA1303 “Sustainable Control of Grapevine Trunk Diseases” for distribution to the identifiable nurseries in each European country. The main objective was to develop understanding of the current propagation practices and to identify those likely to have the greatest impacts on the quality of planting material, especially with regard to the control measures used against fungal trunk pathogen infections. The questionnaire was sent to 666 vine nurseries, and 146 replies were received (21.9% response rate) The study identified several risks factors which could increase infection by fungal trunk pathogens during the propagation processes, as well as a clear need for further research into the effects of treatments on grapevine viability, including hot water treatment, and the potential of biological agents and other strategies such as ozonation to control grapevine trunk diseases in nurseries.