Search

Your search keyword '"van Putten, Jos P M"' showing total 154 results
Start Over Author "van Putten, Jos P M" Publication Type Electronic Resources
154 results on '"van Putten, Jos P M"'

2. MUC13 negatively regulates tight junction proteins and intestinal epithelial barrier integrity via Protein Kinase C

Author

Infectiebiologie, Afd Biomol.Mass Spect. and Proteomics, Pathobiologie, Celbiologie, IOV CCB, Infectious Diseases and Immunology - Infection Biology, Cell Biology, Metabolism & Cancer - Cancer, Cell Biology, Metabolism & Cancer - Cellbiology, Segui-Perez, Celia, Stapels, Daphne A C, Ma, Ziliang, Su, Jinyi, Passchier, Elsemieke, Westendorp, Bart, Wubbolts, Richard W, Wu, Wei, van Putten, Jos P M, Strijbis, Karin, Infectiebiologie, Afd Biomol.Mass Spect. and Proteomics, Pathobiologie, Celbiologie, IOV CCB, Infectious Diseases and Immunology - Infection Biology, Cell Biology, Metabolism & Cancer - Cancer, Cell Biology, Metabolism & Cancer - Cellbiology, Segui-Perez, Celia, Stapels, Daphne A C, Ma, Ziliang, Su, Jinyi, Passchier, Elsemieke, Westendorp, Bart, Wubbolts, Richard W, Wu, Wei, van Putten, Jos P M, and Strijbis, Karin

Published
2024

3. Campylobacter jejuni benefits from the bile salt deoxycholate under low-oxygen condition in a PldA dependent manner

Author

Cao, Xuefeng, van Putten, Jos P M, Wösten, Marc M S M, Cao, Xuefeng, van Putten, Jos P M, and Wösten, Marc M S M

Abstract

Enteric bacteria need to adapt to endure the antibacterial activities of bile salts in the gut. Phospholipase A (PldA) is a key enzyme in the maintenance of bacterial membrane homeostasis. Bacteria respond to stress by modulating their membrane composition. Campylobacter jejuni is the most common cause of human worldwide. However, the mechanism by which C. jejuni adapts and survives in the gut environment is not fully understood. In this study, we investigated the roles of PldA, bile salt sodium deoxycholate (DOC), and oxygen availability in C. jejuni biology, mimicking an in vivo situation. Growth curves were used to determine the adaptation of C. jejuni to bile salts. RNA-seq and functional assays were employed to investigate the PldA-dependent and DOC-induced changes in gene expression that influence bacterial physiology. Survival studies were performed to address oxidative stress defense in C. jejuni. Here, we discovered that PldA of C. jejuni is required for optimal growth in the presence of bile salt DOC. Under high oxygen conditions, DOC is toxic to C. jejuni, but under low oxygen conditions, as is present in the lumen of the gut, C. jejuni benefits from DOC. C. jejuni PldA seems to enable the use of iron needed for optimal growth in the presence of DOC but makes the bacterium more vulnerable to oxidative stress. In conclusion, DOC stimulates C. jejuni growth under low oxygen conditions and alters colony morphology in a PldA-dependent manner. C. jejuni benefits from DOC by upregulating iron metabolism in a PldA-dependent manner.

Published
2023

4. Glycosylated extracellular mucin domains protect against SARS-CoV-2 infection at the respiratory surface

Author

Chatterjee, Maitrayee, Huang, Liane Z X, Mykytyn, Anna Z, Wang, Chunyan, Lamers, Mart M, Westendorp, Bart, Wubbolts, Richard W, van Putten, Jos P M, Bosch, Berend-Jan, Haagmans, Bart L, Strijbis, Karin, Chatterjee, Maitrayee, Huang, Liane Z X, Mykytyn, Anna Z, Wang, Chunyan, Lamers, Mart M, Westendorp, Bart, Wubbolts, Richard W, van Putten, Jos P M, Bosch, Berend-Jan, Haagmans, Bart L, and Strijbis, Karin

Abstract

Mucins play an essential role in protecting the respiratory tract against microbial infections while also acting as binding sites for bacterial and viral adhesins. The heavily O-glycosylated gel-forming mucins MUC5AC and MUC5B eliminate pathogens by mucociliary clearance. Transmembrane mucins MUC1, MUC4, and MUC16 can restrict microbial invasion at the apical surface of the epithelium. In this study, we determined the impact of host mucins and mucin glycans on epithelial entry of SARS-CoV-2. Human lung epithelial Calu-3 cells express the SARS-CoV-2 entry receptor ACE2 and high levels of glycosylated MUC1, but not MUC4 and MUC16, on their cell surface. The O-glycan-specific mucinase StcE specifically removed the glycosylated part of the MUC1 extracellular domain while leaving the underlying SEA domain and cytoplasmic tail intact. StcE treatment of Calu-3 cells significantly enhanced infection with SARS-CoV-2 pseudovirus and authentic virus, while removal of sialic acid and fucose from the epithelial surface did not impact viral entry. In Calu-3 cells, the transmembrane mucin MUC1 and ACE2 are located to the apical surface in close proximity and StcE treatment results in enhanced binding of purified spike protein. Both MUC1 and MUC16 are expressed on the surface of human organoid-derived air-liquid interface (ALI) differentiated airway cultures and StcE treatment led to mucin removal and increased levels of SARS-CoV-2 replication. In these cultures, MUC1 was highly expressed in non-ciliated cells while MUC16 was enriched in goblet cells. In conclusion, the glycosylated extracellular domains of different transmembrane mucins might have similar protective functions in different respiratory cell types by restricting SARS-CoV-2 binding and entry.

Published
2023

6. Glycosylated extracellular mucin domains protect against SARS-CoV-2 infection at the respiratory surface

Author

Infectiebiologie, Virologie, Pathobiologie, Cell Biology, Metabolism & Cancer - Cancer, Infectious Diseases and Immunology - Infection Biology, Infectious Diseases and Immunology - Virology, Cell Biology, Metabolism & Cancer - Cellbiology, Chatterjee, Maitrayee, Huang, Liane Z X, Mykytyn, Anna Z, Wang, Chunyan, Lamers, Mart M, Westendorp, Bart, Wubbolts, Richard W, van Putten, Jos P M, Bosch, Berend-Jan, Haagmans, Bart L, Strijbis, Karin, Infectiebiologie, Virologie, Pathobiologie, Cell Biology, Metabolism & Cancer - Cancer, Infectious Diseases and Immunology - Infection Biology, Infectious Diseases and Immunology - Virology, Cell Biology, Metabolism & Cancer - Cellbiology, Chatterjee, Maitrayee, Huang, Liane Z X, Mykytyn, Anna Z, Wang, Chunyan, Lamers, Mart M, Westendorp, Bart, Wubbolts, Richard W, van Putten, Jos P M, Bosch, Berend-Jan, Haagmans, Bart L, and Strijbis, Karin

Published
2023

8. Campylobacter jejuni permeabilizes the host cell membrane by short chain lysophosphatidylethanolamines

Author

Cao, Xuefeng, van de Lest, Chris H A, Huang, Liane Z X, van Putten, Jos P M, Wösten, Marc M S M, Cao, Xuefeng, van de Lest, Chris H A, Huang, Liane Z X, van Putten, Jos P M, and Wösten, Marc M S M

Abstract

Lysophospholipids (LPLs) are crucial for regulating epithelial integrity and homeostasis in eukaryotes, however the effects of LPLs produced by bacteria on host cells is largely unknown. The membrane of the human bacterial pathogen Campylobacter jejuni is rich in LPLs. Although C. jejuni possesses several virulence factors, it lacks traditional virulence factors like type III secretion systems, present in most enteropathogens. Here, we provide evidence that membrane lipids lysophosphatidylethanolamines (lysoPEs) of C. jejuni are able to lyse erythrocytes and are toxic for HeLa and Caco-2 cells. Lactate dehydrogenase (LDH) release assays and confocal microscopy revealed that lysoPE permeabilizes the cells. LysoPE toxicity was partially rescued by oxidative stress inhibitors, indicating that intracellular reactive oxygen species may contribute to the cell damage. Our results show that especially the short-chain lysoPEs (C:14) which is abundantly present in the C. jejuni membrane may be considered as a novel virulence factor.

Published
2022

10. Campylobacter jejuni permeabilizes the host cell membrane by short chain lysophosphatidylethanolamines

Author

Infectiebiologie, Equine Musculoskeletal Biology, Veterinaire biochemie, CS_Locomotion, Cao, Xuefeng, van de Lest, Chris H A, Huang, Liane Z X, van Putten, Jos P M, Wösten, Marc M S M, Infectiebiologie, Equine Musculoskeletal Biology, Veterinaire biochemie, CS_Locomotion, Cao, Xuefeng, van de Lest, Chris H A, Huang, Liane Z X, van Putten, Jos P M, and Wösten, Marc M S M

Published
2022

12. Campylobacter jejuni permeabilizes the host cell membrane by short chain lysophosphatidylethanolamines

Author

Infectiebiologie, Equine Musculoskeletal Biology, Veterinaire biochemie, CS_Locomotion, Cao, Xuefeng, van de Lest, Chris H A, Huang, Liane Z X, van Putten, Jos P M, Wösten, Marc M S M, Infectiebiologie, Equine Musculoskeletal Biology, Veterinaire biochemie, CS_Locomotion, Cao, Xuefeng, van de Lest, Chris H A, Huang, Liane Z X, van Putten, Jos P M, and Wösten, Marc M S M

Published
2022

15. Naturally circulating pertactin-deficient Bordetella pertussis strains induce distinct gene expression and inflammatory signatures in human dendritic cells

Author

Kroes, Michiel M, Miranda-Bedate, Alberto, Hovingh, Elise S, Jacobi, Ronald, Schot, Corrie, Pupo, Elder, Raeven, René H M, van der Ark, Arno A J, van Putten, Jos P M, de Wit, Jelle, Mariman, Rob, Pinelli, Elena, Kroes, Michiel M, Miranda-Bedate, Alberto, Hovingh, Elise S, Jacobi, Ronald, Schot, Corrie, Pupo, Elder, Raeven, René H M, van der Ark, Arno A J, van Putten, Jos P M, de Wit, Jelle, Mariman, Rob, and Pinelli, Elena

Published
2021

16. Antimicrobial Activities of Alginate and Chitosan Oligosaccharides Against Staphylococcus aureus and Group B Streptococcus

Author

Asadpoor, Mostafa, Ithakisiou, Georgia-Nefeli, van Putten, Jos P M, Pieters, Roland J, Folkerts, Gert, Braber, Saskia, Asadpoor, Mostafa, Ithakisiou, Georgia-Nefeli, van Putten, Jos P M, Pieters, Roland J, Folkerts, Gert, and Braber, Saskia

Abstract

The bacterial pathogens Streptococcus agalactiae (GBS) and Staphylococcus aureus (S. aureus) cause serious infections in humans and animals. The emergence of antibiotic-resistant isolates and bacterial biofilm formation entails the urge of novel treatment strategies. Recently, there is a profound scientific interest in the capabilities of non-digestible oligosaccharides as antimicrobial and anti-biofilm agents as well as adjuvants in antibiotic combination therapies. In this study, we investigated the potential of alginate oligosaccharides (AOS) and chitosan oligosaccharides (COS) as alternative for, or in combination with antibiotic treatment. AOS (2-16%) significantly decreased GBS V growth by determining the minimum inhibitory concentration. Both AOS (8 and 16%) and COS (2-16%) were able to prevent biofilm formation by S. aureus wood 46. A checkerboard biofilm formation assay demonstrated a synergistic effect of COS and clindamycin on the S. aureus biofilm formation, while AOS (2 and 4%) were found to sensitize GBS V to trimethoprim. In conclusion, AOS and COS affect the growth of GBS V and S. aureus wood 46 and can function as anti-biofilm agents. The promising effects of AOS and COS in combination with different antibiotics may offer new opportunities to combat antimicrobial resistance.

Published
2021

17. Identification of Allobaculum mucolyticum as a novel human intestinal mucin degrader

Author

van Muijlwijk, Guus H, van Mierlo, Guido, Jansen, Pascal W T C, Vermeulen, Michiel, Bleumink-Pluym, Nancy M C, Palm, Noah W, van Putten, Jos P M, de Zoete, Marcel R, van Muijlwijk, Guus H, van Mierlo, Guido, Jansen, Pascal W T C, Vermeulen, Michiel, Bleumink-Pluym, Nancy M C, Palm, Noah W, van Putten, Jos P M, and de Zoete, Marcel R

Abstract

The human gut microbiota plays a central role in intestinal health and disease. Yet, many of its bacterial constituents are functionally still largely unexplored. A crucial prerequisite for bacterial survival and proliferation is the creation and/or exploitation of an own niche. For many bacterial species that are linked to human disease, the inner mucus layer was found to be an important niche. Allobaculum mucolyticum is a newly identified, IBD-associated species that is thought be closely associated with the host epithelium. To explore how this bacterium is able to effectively colonize this niche, we screened its genome for factors that may contribute to mucosal colonization. Up to 60 genes encoding putative Carbohydrate Active Enzymes (CAZymes) were identified in the genome of A. mucolyticum. Mass spectrometry revealed 49 CAZymes of which 26 were significantly enriched in its secretome. Functional assays demonstrated the presence of CAZyme activity in A. mucolyticum conditioned medium, degradation of human mucin O-glycans, and utilization of liberated non-terminal monosaccharides for bacterial growth. The results support a model in which sialidases and fucosidases remove terminal O-glycan sugars enabling subsequent degradation and utilization of carbohydrates for A. mucolyticum growth. A. mucolyticum CAZyme secretion may thus facilitate bacterial colonization and degradation of the mucus layer and may pose an interesting target for future therapeutic intervention.

Published
2021

18. The transmembrane mucin muc1 facilitates b1-integrin-mediated bacterial invasion

Author

Li, Xinyue, Wubbolts, Richard W, Bleumink-Pluym, Nancy M C, van Putten, Jos P M, Strijbis, Karin, Li, Xinyue, Wubbolts, Richard W, Bleumink-Pluym, Nancy M C, van Putten, Jos P M, and Strijbis, Karin

Abstract

At the intestinal host-microbe interface, the transmembrane mucin MUC1 can function as a physical barrier as well as a receptor for bacteria. MUC1 also influences epithelial cell morphology and receptor function. Various bacterial pathogens can exploit integrins to infect eukaryotic cells. It is yet unclear whether MUC1 influences the interaction of bacteria with integrins. We used Escherichia coli expressing the invasin (inv) protein of Yersinia pseudotuberculosis (E. coli inv) to assess the effects of MUC1 on b1 integrin (ITGB1)-mediated bacterial invasion. Our results show that expression of full-length MUC1 does not yield a physical barrier but slightly enhances E. coli inv uptake. Enzymatic removal of the MUC1 extracellular domain (ED) using a secreted protease of C1 esterase inhibitor (StcE) of pathogenic Escherichia coli had no additional effect on E. coli inv invasion. In contrast, expression of a truncated MUC1 that lacks the cytoplasmic tail (CT) reduced bacterial entry sub-stantially. Substitution of tyrosine residues in the MUC1 CT also reduced bacterial uptake, while deletion of the C-terminal half of the cytoplasmic tail only had a minor effect, pointing to a regulatory role of tyrosine phosphorylation and the N-terminal region of the MUC1 CT in integrin-mediated uptake process. Unexpectedly, StcE removal of the ED in MUC1-DCT cells reversed the block in bacterial invasion. Together, these findings indicate that MUC1 can facilitate b1-integrin-mediated bacterial invasion by a concerted action of the large glycosylated extracellular domain and the membrane-juxtaposed cytoplasmic tail region. IMPORTANCE Bacteria can exploit membrane receptor integrins for cellular invasion, either by direct binding of bacterial adhesins or utilizing extracellular matrix compo-nents. MUC1 is a large transmembrane glycoprotein expressed by most epithelial cells that can have direct defensive or receptor functions at the host-microbe interface and is involved in facilita

Published
2021

19. Antimicrobial Activities of Alginate and Chitosan Oligosaccharides Against Staphylococcus aureus and Group B Streptococcus

Author

Afd Pharmacology, Infectiebiologie, dI&I I&I-2, Afd Chemical Biology and Drug Discovery, Pharmacology, Chemical Biology and Drug Discovery, Asadpoor, Mostafa, Ithakisiou, Georgia-Nefeli, van Putten, Jos P M, Pieters, Roland J, Folkerts, Gert, Braber, Saskia, Afd Pharmacology, Infectiebiologie, dI&I I&I-2, Afd Chemical Biology and Drug Discovery, Pharmacology, Chemical Biology and Drug Discovery, Asadpoor, Mostafa, Ithakisiou, Georgia-Nefeli, van Putten, Jos P M, Pieters, Roland J, Folkerts, Gert, and Braber, Saskia

Published
2021

20. The ALPK1 pathway drives the inflammatory response to Campylobacter jejuni in human intestinal epithelial cells

Author

LS Infectiebiologie (Bacteriologie), dI&I I&I-2, Infectiebiologie, Cui, Jiannan, Duizer, Coco, Bouwman, Lieneke I, van Rooijen, Kristel S, Voogdt, Carlos G P, van Putten, Jos P M, de Zoete, Marcel R, LS Infectiebiologie (Bacteriologie), dI&I I&I-2, Infectiebiologie, Cui, Jiannan, Duizer, Coco, Bouwman, Lieneke I, van Rooijen, Kristel S, Voogdt, Carlos G P, van Putten, Jos P M, and de Zoete, Marcel R

Published
2021

21. Identification of Allobaculum mucolyticum as a novel human intestinal mucin degrader

Author

dI&I I&I-2, Sub Biomol.Mass Spectrometry & Proteom., Infectiebiologie, van Muijlwijk, Guus H, van Mierlo, Guido, Jansen, Pascal W T C, Vermeulen, Michiel, Bleumink-Pluym, Nancy M C, Palm, Noah W, van Putten, Jos P M, de Zoete, Marcel R, dI&I I&I-2, Sub Biomol.Mass Spectrometry & Proteom., Infectiebiologie, van Muijlwijk, Guus H, van Mierlo, Guido, Jansen, Pascal W T C, Vermeulen, Michiel, Bleumink-Pluym, Nancy M C, Palm, Noah W, van Putten, Jos P M, and de Zoete, Marcel R

Published
2021

22. Naturally circulating pertactin-deficient Bordetella pertussis strains induce distinct gene expression and inflammatory signatures in human dendritic cells

Author

LS Equine Muscoskeletal Biology, Infectiebiologie, dI&I I&I-2, Kroes, Michiel M, Miranda-Bedate, Alberto, Hovingh, Elise S, Jacobi, Ronald, Schot, Corrie, Pupo, Elder, Raeven, René H M, van der Ark, Arno A J, van Putten, Jos P M, de Wit, Jelle, Mariman, Rob, Pinelli, Elena, LS Equine Muscoskeletal Biology, Infectiebiologie, dI&I I&I-2, Kroes, Michiel M, Miranda-Bedate, Alberto, Hovingh, Elise S, Jacobi, Ronald, Schot, Corrie, Pupo, Elder, Raeven, René H M, van der Ark, Arno A J, van Putten, Jos P M, de Wit, Jelle, Mariman, Rob, and Pinelli, Elena

Published
2021

23. The transmembrane mucin muc1 facilitates b1-integrin-mediated bacterial invasion

Author

Infectiebiologie, Celbiologie, IOV CCB, dB&C I&I, dI&I I&I-2, Li, Xinyue, Wubbolts, Richard W, Bleumink-Pluym, Nancy M C, van Putten, Jos P M, Strijbis, Karin, Infectiebiologie, Celbiologie, IOV CCB, dB&C I&I, dI&I I&I-2, Li, Xinyue, Wubbolts, Richard W, Bleumink-Pluym, Nancy M C, van Putten, Jos P M, and Strijbis, Karin

Published
2021

24. Identification of Allobaculum mucolyticum as a novel human intestinal mucin degrader

Author

dI&I I&I-2, Sub Biomol.Mass Spectrometry & Proteom., Infectiebiologie, van Muijlwijk, Guus H, van Mierlo, Guido, Jansen, Pascal W T C, Vermeulen, Michiel, Bleumink-Pluym, Nancy M C, Palm, Noah W, van Putten, Jos P M, de Zoete, Marcel R, dI&I I&I-2, Sub Biomol.Mass Spectrometry & Proteom., Infectiebiologie, van Muijlwijk, Guus H, van Mierlo, Guido, Jansen, Pascal W T C, Vermeulen, Michiel, Bleumink-Pluym, Nancy M C, Palm, Noah W, van Putten, Jos P M, and de Zoete, Marcel R

Published
2021

25. The ALPK1 pathway drives the inflammatory response to Campylobacter jejuni in human intestinal epithelial cells

Author

LS Infectiebiologie (Bacteriologie), dI&I I&I-2, Infectiebiologie, Cui, Jiannan, Duizer, Coco, Bouwman, Lieneke I, van Rooijen, Kristel S, Voogdt, Carlos G P, van Putten, Jos P M, de Zoete, Marcel R, LS Infectiebiologie (Bacteriologie), dI&I I&I-2, Infectiebiologie, Cui, Jiannan, Duizer, Coco, Bouwman, Lieneke I, van Rooijen, Kristel S, Voogdt, Carlos G P, van Putten, Jos P M, and de Zoete, Marcel R

Published
2021

26. Naturally circulating pertactin-deficient Bordetella pertussis strains induce distinct gene expression and inflammatory signatures in human dendritic cells

Author

LS Equine Muscoskeletal Biology, Infectiebiologie, dI&I I&I-2, Kroes, Michiel M, Miranda-Bedate, Alberto, Hovingh, Elise S, Jacobi, Ronald, Schot, Corrie, Pupo, Elder, Raeven, René H M, van der Ark, Arno A J, van Putten, Jos P M, de Wit, Jelle, Mariman, Rob, Pinelli, Elena, LS Equine Muscoskeletal Biology, Infectiebiologie, dI&I I&I-2, Kroes, Michiel M, Miranda-Bedate, Alberto, Hovingh, Elise S, Jacobi, Ronald, Schot, Corrie, Pupo, Elder, Raeven, René H M, van der Ark, Arno A J, van Putten, Jos P M, de Wit, Jelle, Mariman, Rob, and Pinelli, Elena

Published
2021

27. The transmembrane mucin muc1 facilitates b1-integrin-mediated bacterial invasion

Author

Infectiebiologie, Celbiologie, IOV CCB, dB&C I&I, dI&I I&I-2, Li, Xinyue, Wubbolts, Richard W, Bleumink-Pluym, Nancy M C, van Putten, Jos P M, Strijbis, Karin, Infectiebiologie, Celbiologie, IOV CCB, dB&C I&I, dI&I I&I-2, Li, Xinyue, Wubbolts, Richard W, Bleumink-Pluym, Nancy M C, van Putten, Jos P M, and Strijbis, Karin

Published
2021

28. Antimicrobial Activities of Alginate and Chitosan Oligosaccharides Against Staphylococcus aureus and Group B Streptococcus

Author

Afd Pharmacology, Infectiebiologie, dI&I I&I-2, Afd Chemical Biology and Drug Discovery, Pharmacology, Chemical Biology and Drug Discovery, Asadpoor, Mostafa, Ithakisiou, Georgia-Nefeli, van Putten, Jos P M, Pieters, Roland J, Folkerts, Gert, Braber, Saskia, Afd Pharmacology, Infectiebiologie, dI&I I&I-2, Afd Chemical Biology and Drug Discovery, Pharmacology, Chemical Biology and Drug Discovery, Asadpoor, Mostafa, Ithakisiou, Georgia-Nefeli, van Putten, Jos P M, Pieters, Roland J, Folkerts, Gert, and Braber, Saskia

Published
2021

29. Antimicrobial Activities of Alginate and Chitosan Oligosaccharides Against Staphylococcus aureus and Group B Streptococcus

Author

Afd Pharmacology, Infectiebiologie, dI&I I&I-2, Afd Chemical Biology and Drug Discovery, Pharmacology, Chemical Biology and Drug Discovery, Asadpoor, Mostafa, Ithakisiou, Georgia-Nefeli, van Putten, Jos P M, Pieters, Roland J, Folkerts, Gert, Braber, Saskia, Afd Pharmacology, Infectiebiologie, dI&I I&I-2, Afd Chemical Biology and Drug Discovery, Pharmacology, Chemical Biology and Drug Discovery, Asadpoor, Mostafa, Ithakisiou, Georgia-Nefeli, van Putten, Jos P M, Pieters, Roland J, Folkerts, Gert, and Braber, Saskia

Published
2021

30. The ALPK1 pathway drives the inflammatory response to Campylobacter jejuni in human intestinal epithelial cells

Author

LS Infectiebiologie (Bacteriologie), dI&I I&I-2, Infectiebiologie, Cui, Jiannan, Duizer, Coco, Bouwman, Lieneke I, van Rooijen, Kristel S, Voogdt, Carlos G P, van Putten, Jos P M, de Zoete, Marcel R, LS Infectiebiologie (Bacteriologie), dI&I I&I-2, Infectiebiologie, Cui, Jiannan, Duizer, Coco, Bouwman, Lieneke I, van Rooijen, Kristel S, Voogdt, Carlos G P, van Putten, Jos P M, and de Zoete, Marcel R

Published
2021

31. Identification of Allobaculum mucolyticum as a novel human intestinal mucin degrader

Author

dI&I I&I-2, Sub Biomol.Mass Spectrometry & Proteom., Infectiebiologie, van Muijlwijk, Guus H, van Mierlo, Guido, Jansen, Pascal W T C, Vermeulen, Michiel, Bleumink-Pluym, Nancy M C, Palm, Noah W, van Putten, Jos P M, de Zoete, Marcel R, dI&I I&I-2, Sub Biomol.Mass Spectrometry & Proteom., Infectiebiologie, van Muijlwijk, Guus H, van Mierlo, Guido, Jansen, Pascal W T C, Vermeulen, Michiel, Bleumink-Pluym, Nancy M C, Palm, Noah W, van Putten, Jos P M, and de Zoete, Marcel R

Published
2021

32. Naturally circulating pertactin-deficient Bordetella pertussis strains induce distinct gene expression and inflammatory signatures in human dendritic cells

Author

LS Equine Muscoskeletal Biology, Infectiebiologie, dI&I I&I-2, Kroes, Michiel M, Miranda-Bedate, Alberto, Hovingh, Elise S, Jacobi, Ronald, Schot, Corrie, Pupo, Elder, Raeven, René H M, van der Ark, Arno A J, van Putten, Jos P M, de Wit, Jelle, Mariman, Rob, Pinelli, Elena, LS Equine Muscoskeletal Biology, Infectiebiologie, dI&I I&I-2, Kroes, Michiel M, Miranda-Bedate, Alberto, Hovingh, Elise S, Jacobi, Ronald, Schot, Corrie, Pupo, Elder, Raeven, René H M, van der Ark, Arno A J, van Putten, Jos P M, de Wit, Jelle, Mariman, Rob, and Pinelli, Elena

Published
2021

33. The transmembrane mucin muc1 facilitates b1-integrin-mediated bacterial invasion

Author

Infectiebiologie, Celbiologie, IOV CCB, dI&I I&I-2, Infectious Diseases and Immunology - Infection Biology, Li, Xinyue, Wubbolts, Richard W, Bleumink-Pluym, Nancy M C, van Putten, Jos P M, Strijbis, Karin, Infectiebiologie, Celbiologie, IOV CCB, dI&I I&I-2, Infectious Diseases and Immunology - Infection Biology, Li, Xinyue, Wubbolts, Richard W, Bleumink-Pluym, Nancy M C, van Putten, Jos P M, and Strijbis, Karin

Published
2021

34. Identification of Allobaculum mucolyticum as a novel human intestinal mucin degrader

Author

MMB Research line 2, Infection & Immunity, van Muijlwijk, Guus H, van Mierlo, Guido, Jansen, Pascal W T C, Vermeulen, Michiel, Bleumink-Pluym, Nancy M C, Palm, Noah W, van Putten, Jos P M, de Zoete, Marcel R, MMB Research line 2, Infection & Immunity, van Muijlwijk, Guus H, van Mierlo, Guido, Jansen, Pascal W T C, Vermeulen, Michiel, Bleumink-Pluym, Nancy M C, Palm, Noah W, van Putten, Jos P M, and de Zoete, Marcel R

Published
2021

36. The Unique Phospholipidome of the Enteric Pathogen Campylobacter jejuni: Lysophosholipids Are Required for Motility at Low Oxygen Availability

Author

Cao, Xuefeng, Brouwers, Jos F H M, van Dijk, Linda, van de Lest, Chris H A, Parker, Craig T, Huynh, Steven, van Putten, Jos P M, Kelly, David J, Wösten, Marc M S M, Cao, Xuefeng, Brouwers, Jos F H M, van Dijk, Linda, van de Lest, Chris H A, Parker, Craig T, Huynh, Steven, van Putten, Jos P M, Kelly, David J, and Wösten, Marc M S M

Abstract

In response to changes in their environment bacteria need to change both their protein and phospholipid repertoire to match environmental requirements, but the dynamics of bacterial phospholipid composition under different growth conditions is still largely unknown. In the present study, we investigated the phospholipidome of the bacterial pathogen Campylobacter jejuni. Transcription profiling on logarithmic and stationary phase grown cells of the microaerophilic human pathogen C. jejuni using RNA-seq revealed differential expression of putative phospholipid biosynthesis genes. By applying high-performance liquid chromatography tandem-mass spectrometry, we identified 203 phospholipid species representing the first determination of the phospholipidome of this pathogen. We identified nine different phospholipid classes carrying between one and three acyl chains. Phospholipidome analysis on bacteria of different ages (0-5 days) showed rapid changes in the ratio of phospholipids containing ethanolamine, or glycerol as phospholipid head group and in the number of cyclopropane bond containing fatty acids. Oxygen concentration influenced the percentage of lysophospholipids, and cyclo-propane bonds containing acyl chains. We show that large amounts of the phospholipids are lysophospholipids (30-45%), which mutant studies reveal are needed for normal C. jejuni motility at low oxygen conditions. C. jejuni possesses an unusual phospholipidome that is highly dynamic in response to environmental changes.

Published
2020

37. The Unique Phospholipidome of the Enteric Pathogen Campylobacter jejuni: Lysophosholipids Are Required for Motility at Low Oxygen Availability

Author

dB&C FR-RMSC FR, dB&C FR-RMSC RMSC, Equine Musculoskeletal Biology, Veterinaire biochemie, dES RMSC, Infectiebiologie, dI&I I&I-2, Cao, Xuefeng, Brouwers, Jos F H M, van Dijk, Linda, van de Lest, Chris H A, Parker, Craig T, Huynh, Steven, van Putten, Jos P M, Kelly, David J, Wösten, Marc M S M, dB&C FR-RMSC FR, dB&C FR-RMSC RMSC, Equine Musculoskeletal Biology, Veterinaire biochemie, dES RMSC, Infectiebiologie, dI&I I&I-2, Cao, Xuefeng, Brouwers, Jos F H M, van Dijk, Linda, van de Lest, Chris H A, Parker, Craig T, Huynh, Steven, van Putten, Jos P M, Kelly, David J, and Wösten, Marc M S M

Published
2020

40. The Unique Phospholipidome of the Enteric Pathogen Campylobacter jejuni: Lysophosholipids Are Required for Motility at Low Oxygen Availability

Author

dB&C FR-RMSC FR, dB&C FR-RMSC RMSC, Equine Musculoskeletal Biology, Veterinaire biochemie, dES RMSC, Infectiebiologie, dI&I I&I-2, Cao, Xuefeng, Brouwers, Jos F H M, van Dijk, Linda, van de Lest, Chris H A, Parker, Craig T, Huynh, Steven, van Putten, Jos P M, Kelly, David J, Wösten, Marc M S M, dB&C FR-RMSC FR, dB&C FR-RMSC RMSC, Equine Musculoskeletal Biology, Veterinaire biochemie, dES RMSC, Infectiebiologie, dI&I I&I-2, Cao, Xuefeng, Brouwers, Jos F H M, van Dijk, Linda, van de Lest, Chris H A, Parker, Craig T, Huynh, Steven, van Putten, Jos P M, Kelly, David J, and Wösten, Marc M S M

Published
2020

41. The Unique Phospholipidome of the Enteric Pathogen Campylobacter jejuni: Lysophosholipids Are Required for Motility at Low Oxygen Availability

Author

dB&C FR-RMSC FR, dB&C FR-RMSC RMSC, Equine Musculoskeletal Biology, Veterinaire biochemie, dES RMSC, Infectiebiologie, dI&I I&I-2, Cao, Xuefeng, Brouwers, Jos F H M, van Dijk, Linda, van de Lest, Chris H A, Parker, Craig T, Huynh, Steven, van Putten, Jos P M, Kelly, David J, Wösten, Marc M S M, dB&C FR-RMSC FR, dB&C FR-RMSC RMSC, Equine Musculoskeletal Biology, Veterinaire biochemie, dES RMSC, Infectiebiologie, dI&I I&I-2, Cao, Xuefeng, Brouwers, Jos F H M, van Dijk, Linda, van de Lest, Chris H A, Parker, Craig T, Huynh, Steven, van Putten, Jos P M, Kelly, David J, and Wösten, Marc M S M

Published
2020

44. MUC1 is a receptor for the Salmonella SiiE adhesin that enables apical invasion into enterocytes

Author

Li, Xinyue, Bleumink-Pluym, Nancy M. C., Luijkx, Yvette M. C. A., Wubbolts, Richard W., van Putten, Jos P. M., Strijbis, Karin, Li, Xinyue, Bleumink-Pluym, Nancy M. C., Luijkx, Yvette M. C. A., Wubbolts, Richard W., van Putten, Jos P. M., and Strijbis, Karin

Abstract

The cellular invasion machinery of the enteric pathogen Salmonella consists of a type III secretion system (T3SS) with injectable virulence factors that induce uptake by macropinocytosis. Salmonella invasion at the apical surface of intestinal epithelial cells is inefficient, presumably because of a glycosylated barrier formed by transmembrane mucins that prevents T3SS contact with host cells. We observed that Salmonella is capable of apical invasion of intestinal epithelial cells that express the transmembrane mucin MUC1. Knockout of MUC1 in HT29-MTX cells or removal of MUC1 sialic acids by neuraminidase treatment reduced Salmonella apical invasion but did not affect lateral invasion that is not hampered by a defensive barrier. A Salmonella deletion strain lacking the SiiE giant adhesin was unable to invade intestinal epithelial cells through MUC1. SiiE-positive Salmonella closely associated with the MUC1 layer at the apical surface, but invaded Salmonella were negative for the adhesin. Our findings uncover that the transmembrane mucin MUC1 is required for Salmonella SiiE-mediated entry of enterocytes via the apical route.

Published
2019

45. Activation of Human NK Cells by Bordetella pertussis Requires Inflammasome Activation in Macrophages

Author

Faculteit Diergeneeskunde, LS Infectiebiologie (Bacteriologie), dI&I I&I-2, Kroes, Michiel M, Mariman, Rob, Hijdra, Daniëlle, Hamstra, Hendrik-Jan, van Boxtel, Karlijn J W M, van Putten, Jos P M, de Wit, Jelle, Pinelli, Elena, Faculteit Diergeneeskunde, LS Infectiebiologie (Bacteriologie), dI&I I&I-2, Kroes, Michiel M, Mariman, Rob, Hijdra, Daniëlle, Hamstra, Hendrik-Jan, van Boxtel, Karlijn J W M, van Putten, Jos P M, de Wit, Jelle, and Pinelli, Elena

Published
2019

46. MUC1 is a receptor for the Salmonella SiiE adhesin that enables apical invasion into enterocytes

Author

dI&I I&I-2, LS Infectiebiologie (Bacteriologie), Faculteit Diergeneeskunde, dB&C I&I, LS Celbiologie-Algemeen, Sub Center for Cell Imaging, Afd Chemical Biology and Drug Discovery, Li, Xinyue, Bleumink-Pluym, Nancy M. C., Luijkx, Yvette M. C. A., Wubbolts, Richard W., van Putten, Jos P. M., Strijbis, Karin, dI&I I&I-2, LS Infectiebiologie (Bacteriologie), Faculteit Diergeneeskunde, dB&C I&I, LS Celbiologie-Algemeen, Sub Center for Cell Imaging, Afd Chemical Biology and Drug Discovery, Li, Xinyue, Bleumink-Pluym, Nancy M. C., Luijkx, Yvette M. C. A., Wubbolts, Richard W., van Putten, Jos P. M., and Strijbis, Karin

Published
2019

47. Activation of Human NK Cells by Bordetella pertussis Requires Inflammasome Activation in Macrophages

Author

Faculteit Diergeneeskunde, LS Infectiebiologie (Bacteriologie), dI&I I&I-2, Kroes, Michiel M, Mariman, Rob, Hijdra, Daniëlle, Hamstra, Hendrik-Jan, van Boxtel, Karlijn J W M, van Putten, Jos P M, de Wit, Jelle, Pinelli, Elena, Faculteit Diergeneeskunde, LS Infectiebiologie (Bacteriologie), dI&I I&I-2, Kroes, Michiel M, Mariman, Rob, Hijdra, Daniëlle, Hamstra, Hendrik-Jan, van Boxtel, Karlijn J W M, van Putten, Jos P M, de Wit, Jelle, and Pinelli, Elena

Published
2019

48. MUC1 is a receptor for the Salmonella SiiE adhesin that enables apical invasion into enterocytes

Author

dI&I I&I-2, LS Infectiebiologie (Bacteriologie), Faculteit Diergeneeskunde, dB&C I&I, LS Celbiologie-Algemeen, Sub Center for Cell Imaging, Afd Chemical Biology and Drug Discovery, Li, Xinyue, Bleumink-Pluym, Nancy M. C., Luijkx, Yvette M. C. A., Wubbolts, Richard W., van Putten, Jos P. M., Strijbis, Karin, dI&I I&I-2, LS Infectiebiologie (Bacteriologie), Faculteit Diergeneeskunde, dB&C I&I, LS Celbiologie-Algemeen, Sub Center for Cell Imaging, Afd Chemical Biology and Drug Discovery, Li, Xinyue, Bleumink-Pluym, Nancy M. C., Luijkx, Yvette M. C. A., Wubbolts, Richard W., van Putten, Jos P. M., and Strijbis, Karin

Published
2019

49. Activation of Human NK Cells by Bordetella pertussis Requires Inflammasome Activation in Macrophages

Author

Faculteit Diergeneeskunde, LS Infectiebiologie (Bacteriologie), dI&I I&I-2, Kroes, Michiel M, Mariman, Rob, Hijdra, Daniëlle, Hamstra, Hendrik-Jan, van Boxtel, Karlijn J W M, van Putten, Jos P M, de Wit, Jelle, Pinelli, Elena, Faculteit Diergeneeskunde, LS Infectiebiologie (Bacteriologie), dI&I I&I-2, Kroes, Michiel M, Mariman, Rob, Hijdra, Daniëlle, Hamstra, Hendrik-Jan, van Boxtel, Karlijn J W M, van Putten, Jos P M, de Wit, Jelle, and Pinelli, Elena

Published
2019

50. MUC1 is a receptor for the Salmonella SiiE adhesin that enables apical invasion into enterocytes

Author

dI&I I&I-2, LS Infectiebiologie (Bacteriologie), Afd Chemical Biology and Drug Discovery, IOV CCB, Celbiologie, Infectiebiologie, Infectious Diseases and Immunology - Infection Biology, Li, Xinyue, Bleumink-Pluym, Nancy M. C., Luijkx, Yvette M. C. A., Wubbolts, Richard W., van Putten, Jos P. M., Strijbis, Karin, dI&I I&I-2, LS Infectiebiologie (Bacteriologie), Afd Chemical Biology and Drug Discovery, IOV CCB, Celbiologie, Infectiebiologie, Infectious Diseases and Immunology - Infection Biology, Li, Xinyue, Bleumink-Pluym, Nancy M. C., Luijkx, Yvette M. C. A., Wubbolts, Richard W., van Putten, Jos P. M., and Strijbis, Karin

Published
2019

Catalog

Books, media, physical & digital resources
See catalog results