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Today the environmental issues are a lot on the agenda and the environmental awareness are more and more common. New laws and restrictions on engines emissions are enforced and the demand on the engines gets higher and tougher. This leads to the engine testing playing a more crucial part than ever. Engine tests are done using an engine dynamometer. The dynamometer loads the engine by, in many different ways, absorbing the power and torque generated by engine. The most important functions of a dyno are to convey the power from the engine to the dyno, to load the engine, to measure the power and torque generated by the engine and to remove the excess heat that is generated. Husqvarna is a Swedish company that produces garden and forest cutting tools, their most famous products are their chainsaws. Husqvarna does an extensive amount of engine testing, long time testing, functional testing and field testing. Some functional tests, such as start-ability after use is done in a climate chamber where the humidity and temperature can be set. Today, loading a chainsaw in -25 °C is a problem due to the fact that the most used dynamometer at Husqvarna is a water brake that freezes in minus degrees. This master thesis will answer the question on how to, for small engine, simulate the load that occurs during normal use of the engine and how to develop a dynamometer suitable for Husqvarna’s needs? The focus when developing the dynamometer will lie on solving the problem with minus degrees and having a good detachable coupling between the dyno and the chainsaw. The result is a hydraulic oil dynamometer. A hydraulic pump is attached to the guide bar and chainsaw using a detachable key way coupling and bearings. By controlling restriction of the flow in the hydraulic system the load can be controlled and also ultimately the RPM of the chainsaw. The hydraulic oil works fine in the minus degree as long as the right oil with the right viscosity range is used although a problem with the

Today the environmental issues are a lot on the agenda and the environmental awareness are more and more common. New laws and restrictions on engines emissions are enforced and the demand on the engines gets higher and tougher. This leads to the engine testing playing a more crucial part than ever. Engine tests are done using an engine dynamometer. The dynamometer loads the engine by, in many different ways, absorbing the power and torque generated by engine. The most important functions of a dyno are to convey the power from the engine to the dyno, to load the engine, to measure the power and torque generated by the engine and to remove the excess heat that is generated. Husqvarna is a Swedish company that produces garden and forest cutting tools, their most famous products are their chainsaws. Husqvarna does an extensive amount of engine testing, long time testing, functional testing and field testing. Some functional tests, such as start-ability after use is done in a climate chamber where the humidity and temperature can be set. Today, loading a chainsaw in -25 °C is a problem due to the fact that the most used dynamometer at Husqvarna is a water brake that freezes in minus degrees. This master thesis will answer the question on how to, for small engine, simulate the load that occurs during normal use of the engine and how to develop a dynamometer suitable for Husqvarna’s needs? The focus when developing the dynamometer will lie on solving the problem with minus degrees and having a good detachable coupling between the dyno and the chainsaw. The result is a hydraulic oil dynamometer. A hydraulic pump is attached to the guide bar and chainsaw using a detachable key way coupling and bearings. By controlling restriction of the flow in the hydraulic system the load can be controlled and also ultimately the RPM of the chainsaw. The hydraulic oil works fine in the minus degree as long as the right oil with the right viscosity range is used although a problem with the

© 2019, Springer Nature Switzerland AG. Psychrotolerant microbes are crucial for carbon cycling and biotechnological applications. Nonetheless, the mechanisms enabling their survival and functioning in frozen environments remain unclear. To elucidate adaptations of microbial cell membranes to freezing, we incubated soils with position-specific 13C labeled glucose at + 5 (control), − 5 and − 20 °C and quantified 13C in CO2 and phospholipid fatty acids. High oxidation of glucose C-1 at + 5 °C revealed a transformation via the pentose phosphate pathway. At subzero temperatures, however, the preferential oxidation of C-4 position suggested a switch to glycolysis. The threefold increase of Gram-negative phospholipid fatty acids in soil incubated at − 5 °C was accompanied by a twofold increase in 13C incorporation. This unequal increase of phospholipid fatty acids and incorporated 13C can be explained by simultaneous desaturation of existing fatty acid chains and the de novo synthesis of monounsaturated fatty acids, which indicates microbial growth. In contrast, Gram-positive bacteria incorporated 2 times higher 13C into their phospholipid fatty acids at − 20 °C than at − 5 and + 5 °C without a significant increase in their fatty acid contents. This reflects intensive repair of membranes damaged at − 20 °C without microbial growth. The fungal/bacterial ratio was 1.5 times lower at subzero temperatures than at + 5 °C, reflecting a shift in microbial community structure towards bacteria. Accordingly, soil microorganisms adapted to freezing by (1) switching their metabolic pathway from the pentose phosphate pathway to glycolysis, (2) modifying phospholipid fatty acids by desaturation and, (3) shifting microbial community structure towards Gram-negative bacteria by reducing the fungal population.