1. Multicenter inter-laboratory analysis of routine susceptibility testing with a challenge panel of resistant strains.
- Author
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UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Laboratoire de biologie clinique, UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, UCL - (SLuc) Service de microbiologie, Deckers, Corentin, Soleimani, Reza, Denis, Olivier, Bogaerts, Pierre, BERHIN, Catherine, Rodríguez-Villalobos, Hector, Descy, Julie, Hallin, Marie, Nonhoff, Claire, Desmet, Stefanie, Magerman, Koen, Vanden Abeele, Anne Marie, Lissoir, Bénédicte, Matheeussen, Veerle, Vernelen, Kris, Huang, Te-Din, Belgian National Antibiogram Committee, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Laboratoire de biologie clinique, UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, UCL - (SLuc) Service de microbiologie, Deckers, Corentin, Soleimani, Reza, Denis, Olivier, Bogaerts, Pierre, BERHIN, Catherine, Rodríguez-Villalobos, Hector, Descy, Julie, Hallin, Marie, Nonhoff, Claire, Desmet, Stefanie, Magerman, Koen, Vanden Abeele, Anne Marie, Lissoir, Bénédicte, Matheeussen, Veerle, Vernelen, Kris, Huang, Te-Din, and Belgian National Antibiogram Committee
- Abstract
To elaborate a new national challenge panel of resistant GNB/GPC strains for the validation of routine antimicrobial susceptibility testing (AST) methods, an interlaboratory evaluation was organized. Results of 12 well-characterized MDR strains tested by 9 laboratories using local disk diffusion (DD) and automated AST (AUST) methods were compared to the reference broth microdilution. Overall categorical agreements (CA) ranged from 70% to 100% for both DD and AUST and were > 90% for all but one strain for all antibiotics. Our multicenter AST study showed good reproducibility and the panel can be used as national resistant reference strains for routine AST validation.
- Published
- 2022