1. 5-Fluorouracil Combined with Rutaecarpine Synergistically Suppresses the Growth of Colon Cancer Cells by Inhibiting STAT3
- Author
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Yu,Zhen, Chan,Shixin, Wang,Xu, Sun,Rui, Wang,Ming, Wang,Zhenglin, Zuo,Xiaomin, Chen,Jiajie, Zhang,Huabing, Chen,Wei, Yu,Zhen, Chan,Shixin, Wang,Xu, Sun,Rui, Wang,Ming, Wang,Zhenglin, Zuo,Xiaomin, Chen,Jiajie, Zhang,Huabing, and Chen,Wei
- Abstract
Zhen Yu,1,* Shixin Chan,2,* Xu Wang,2 Rui Sun,2 Ming Wang,1 Zhenglin Wang,2 Xiaomin Zuo,2 Jiajie Chen,3 Huabing Zhang,4 Wei Chen1,2,5 1Department of General Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Peopleâs Republic of China; 2Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Peopleâs Republic of China; 3Department of Dermatology, First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Peopleâs Republic of China; 4Department of Biochemistry & Molecular Biology, School of Basic Medicine, Anhui Medical University, Hefei, 230032, Peopleâs Republic of China; 5Anhui Provincial Institute of Translational Medicine, Hefei, 230022, Peopleâs Republic of China*These authors contributed equally to this workCorrespondence: Huabing Zhang, Department of Biochemistry & Molecular Biology, School of Basic Medicine, Anhui Medical University, Hefei, 230032, Peopleâs Republic of China, Email slzhang1977@163.com; huabingzhang@ahmu.edu.cn Wei Chen, Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, Peopleâs Republic of China, Tel +86 13966711566, Fax +86 0551-63633742, Email chenwei366@126.com; chenwei366@ahmu.edu.cnPurpose: To evaluate the effect of 5-fluorouracil (5-FU) combined with rutaecarpine (RUT) on the antiproliferative, anti-migratory, and apoptosis-promoting ability of colorectal cancer (CRC) cells and explore the underlying mechanism.Methods: The antiproliferative effects of RUT and 5-FU on CRC cells were evaluated using MTT and colony formation assays. Anti-migration was assessed by cell scratch and transwell tests. The synergistic effect of RUT and 5-FU was assessed by isobologram and combination index analysis using CompuSyn software. The effects of RUT and 5-FU on cell apoptosis were detected by flow cytometry. Differences in protein expression levels with o
- Published
- 2023