Search

Tingting Xu,1,* Wei Sun,2,* Hongqing Zhao,3,* Xinmin Wang,4 Qi Yuan,1 Xijie Zhang,1 Shan Mao,5 Xiuwei Zhang,6 Mingming Zhao,7 Zebo Sheng,8 Mingshun Zhang,9 Mao Huang,1 Ningfei Ji1 1Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of China; 2Department of Respiratory and Critical Care Medicine, Xishan People’s Hospital of Wuxi City, Wuxi, People’s Republic of China; 3Department of Respiratory and Critical Care Medicine, Wuxi Second People’s Hospital, Wuxi, People’s Republic of China; 4Department of Respiratory and Critical Care Medicine, Shuyang Hospital of Traditional Chinese Medicine, Suqian, People’s Republic of China; 5Department of Respiratory and Critical Care Medicine, Nanjing First Hospital, Nanjing, People’s Republic of China; 6Department of Respiratory and Critical Care Medicine, Nanjing Jiangning Hospital, Nanjing, People’s Republic of China; 7Department of Respiratory and Critical Care Medicine, Nanjing Gaochun People’s Hospital, Nanjing, People’s Republic of China; 8Department of Respiratory and Critical Care Medicine, Changshu First People’s Hospital, Suzhou, People’s Republic of China; 9Department of Immunology, Nanjing Medical University, Nanjing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Mao Huang; Ningfei Ji, Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Nanjing Medical University, No. 300, Guangzhou Road, Nanjing, 210029, People’s Republic of China, Tel/Fax +86-13813886116 ; +86-18951080699, Email hm6114@163.com; jiningfei@163.comPurpose: Hospitalization for acute exacerbations of chronic obstructive pulmonary disease (AECOPD) is considered as severe exacerbations. Readmission for severe exacerbations is a crucial event for COPD patients. However, factors associated with readmission for severe exacerbations are

Wenqing Yu,1,* Ningfei Ji,2,* Chengjing Gu,3,* Juan Yao,4 Mingdong Ding,1 Daming Zhou,1 Mao Huang,2 Mingshun Zhang5,6 1Department of Infectious Diseases, Taizhou People’s Hospital Affiliated to Nantong University, Taizhou, Jiangsu, 225300, People’s Republic of China; 2Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, People’s Republic of China; 3Department of Pharmacy, Taizhou People’s Hospital Affiliated to Nantong University, Taizhou, Jiangsu, 225300, People’s Republic of China; 4Department of Oncology, Huaian Hospital of Huaian City, Huaian, Jiangsu, 223200, People’s Republic of China; 5Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu, 211166, People’s Republic of China; 6NHC Key Laboratory of Antibody Technique, Nanjing Medical University, Nanjing, Jiangsu, 211166, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chengjing GuDepartment of Pharmacy, Taizhou People’s Hospital Affiliated to Nantong University, Taizhou, Jiangsu, 225300, People’s Republic of ChinaTel +86 0523-86606290Email chengjingyu706@163.comWenqing YuDepartment of Infectious Diseases, Taizhou People’s Hospital Affiliated to Nantong University, Taizhou, Jiangsu, 225300, People’s Republic of ChinaEmail wenqinggu706@126.comBackground: RORγt+Foxp3+ (Th17-like) Tregs are a plastic Treg subset implicated in immune-related diseases; however, the mechanism of Treg phenotypic transformation in malignant pleural effusion (MPE) has not been elucidated.Methods: The percentage of CD4+CD25+Foxp3+Helios+ and RORγt+Foxp3+ Tregs from peripheral blood and pleural effusion mononuclear cells were measured. The level of interferon regulatory factor 4 (IRF4) mRNA expression was detected by quantitative real-time reverse transcription polymerase chain reaction. The effects of IRF4 on the induction of Tregs from

Practices and research on measuring traditionally urban sustainability abound, therefore the challenge now is related to how the urban carbon issues are included into current measuring methods, thus there is a need to develop methods for measuring urban low-carbon sustainability. In this paper, a simple method, which is based on low-carbon sustainability index, is developed. The overall urban low-carbon sustainability index is the weighted sum of 11 single indices, and each single index is defined as the indicator assessing the development level against the baseline. The baseline is often the criteria or the minimum requirement of low-carbon sustainability. Case studies in four Chinese cities have put this method into practice, and the results show that all four selected cities fail to pass the testing of sensible low-carbon sustainability rule and they are all in weakly low-carbon sustainable development. Although the four cities have made great progress in their capacity building on pollution control and their capacities on wastewater treatment, main pollutants' removal and household and hazardous wastes treatment are enough to meet the needs of local development, they are all facing the great challenges on using of sustainable energy, offsetting of CO2 emissions and adoptions of nature-based solutions. The method developed by this research is a useful tool for decision makers identifying whether the local development is not on a low-carbon sustainable path.

Monocytes exist in two major populations, termed Ly6C^hi and Ly6C^low monocytes. Compared to Ly6C^hi monocytes, less is known about Ly6C^low monocyte recruitment and mechanisms involved in the recruitment of this subset. Furthermore, the role of Ly6C^low monocytes during infections is largely unknown. Here, using intravital microscopy, we demonstrate that Ly6C^low monocytes are predominantly recruited to the brain vasculature following intravenous infection with Cryptococcus neoformans, a fungal pathogen causing meningoencephalitis. The recruitment depends primarily on the interaction of VCAM1 expressed on the brain endothelium with VLA4 expressed on Ly6C^low monocytes. Furthermore, TNFR signaling is essential for the recruitment through enhancing VLA4 expression on Ly6C^low monocytes. Interestingly, the recruited Ly6C^low monocytes internalized C. neoformans and carried the organism while crawling on and adhering to the luminal wall of brain vasculature and migrating to the brain parenchyma. Our study reveals a substantial recruitment of Ly6C^low monocytes to the brain and highlights important properties of this subset during infection.

Energy service companies (ESCOs) play crucial role in building energy efficiency retrofit sector. However limited access to green financing has prevented ESCOs in their expansions in China. This paper, based on a survey of 469 samples and on-site visiting to and interviewing relevant 50 actors of ESCOs, financial institutions and local housing authorities, identifies main barriers of accessing to green financing at both systemic policy level and operational meso and micro level in China, and analyzes good practices at local level that overcome the barriers. The paper concludes that, although there are barriers existing at the policy level in China, substantial attentions and priorities should be given to take actions for overcoming the barriers existed at the operational meso and micro level. The paper suggests that the good practices of capacity building for ESCOs and local financial sector, intensifying participation of intermediate organizations or facilitators and diversifying financial sources and funding mechanisms and models that emerge from the local level should be disseminated in China.

Fungal dissemination into the bloodstream is a critical step leading to invasive fungal infections. Here, using intravital imaging, we show that Kupffer cells (KCs) in the liver have a prominent function in the capture of circulating Cryptococcus neoformans and Candida albicans, thereby reducing fungal dissemination to target organs. Complement C3 but not C5, and complement receptor CRIg but not CR3, are involved in capture of C. neoformans. Internalization of C. neoformans by KCs is subsequently mediated by multiple receptors, including CR3, CRIg, and scavenger receptors, which work synergistically along with C5aR signaling. Following phagocytosis, the growth of C. neoformans is inhibited by KCs in an IFN-γ independent manner. Thus, the liver filters disseminating fungi from circulation via KCs, providing a mechanistic explanation for the enhanced risk of cryptococcosis among individuals with liver diseases, and suggesting a therapeutic strategy to prevent fungal dissemination through enhancing KC functions.

China's regular energy statistics does not include the building sector, and data on building energy demand is included in other types of energy consumption in the Energy Balance Sheet (EBS). Therefore data on building energy demand is not collected based on statistics, but rather calculated or estimated by various approaches in China. This study aims at developing and testing China's building energy statistics by applying an adapted EBS. The advantage of the adapted EBS is that statistical data is from the regular statistical system and no additional statistical efforts are needed. The research result shows that the adapted EBS can be included in China regular energy statistical system and can be standardized in a transparent way. Testing of the adapted EBS shows that China's building energy demand has shown an annual increase of 7.6% since 2001, and a lower contribution to the total energy demand as compared to the developed world. There is also a close link to lifestyle and living standard while industrial energy demand is mainly driven by economy and decoupling of building energy demand with increasing of building floor area, this is due to a considerable improvement of building energy efficiency. The adapted EBS creates a method for China conducting statistics of building energy consumption at the sector level in a uniform way and serves as the basis for any sound building energy efficiency policy decisions.

A recent evaluation of the Better Mills Initiative in China reveal that brands, mills and sector associations agree the initiative was important, given the tightened environmental laws and the value of addressing environmental risks. A narrow scope, clear performance measures and targets and a strategic approach is vital to the success of such an initiative.

A recent evaluation of the Better Mills Initiative in China reveal that brands, mills and sector associations agree the initiative was important, given the tightened environmental laws and the value of addressing environmental risks. A narrow scope, clear performance measures and targets and a strategic approach is vital to the success of such an initiative.

A recent evaluation of the Better Mills Initiative in China reveal that brands, mills and sector associations agree the initiative was important, given the tightened environmental laws and the value of addressing environmental risks. A narrow scope, clear performance measures and targets and a strategic approach is vital to the success of such an initiative.

This paper reports on a nationwide field survey of managing energy efficiency of buildings under energy performance contracting (EPC) in Chinese building sector. The survey aims at getting insight of Chinese experiences of EPC and survey yielded information on profile, specificity and risk specifications of EPC in Chinese building sector. The key findings are that the existing EPC projects are mainly driven by policies and majority of first parties in EPC are owners of public buildings. The contract specificity is worryingly low, with underspecification prominent in the contract sections of renewal and change of the planned solutions, dispute resolution and compensation for personal and property damage. Insufficient risk specification was a major cause of contract failure and disputing. High risks are observed in not enough feasibility study, delay in completion, operational risks, delay in payment and uninsured loss. Most post EPC projects would be worryingly unsuccessful, given to the facts that many of them have not established their energy team, have no further investment and have no effective maintenance. The Chinese existing emission trading scheme (ETS) offers a vital opportunity for upscaling EPC in building sector and policy framing is needed for linking EPC projects and ETS.

Chemokines and chemokine receptors cooperate to promote immune cell recruitment to the central nervous system (CNS). In this study, we investigated the roles of CXCR2 and CXCL1 in leukocyte recruitment to the CNS using a murine model of neuroinflammation. Wild-type (WT), CXCL1−/−, and CXCR2−/− mice each received an intracerebroventricular (i.c.v.) injection of lipopolysaccharide (LPS). Esterase staining and intravital microscopy were performed to examine neutrophil recruitment to the brain. To assess endothelial activation in these mice, the expression of adhesion molecules was measured via quantitative real-time polymerase chain reaction (PCR) and Western blotting. To identify the cellular source of functional CXCR2, chimeric mice were generated by transferring bone marrow cells between the WT and CXCR2−/− mice. Expression levels of the chemokines CXCL1, CXCL2, and CXCL5 were significantly increased in the brain following the i.c.v. injection of LPS. CXCR2 or CXCL1 deficiency blocked neutrophil infiltration and leukocyte recruitment in the cerebral microvessels. In the CXCR2−/− and CXCL1−/− mice, the cerebral endothelial expression of adhesion molecules such as P-selectin and VCAM-1 was dramatically reduced. Furthermore, the bone marrow transfer experiments demonstrated that CXCR2 expression on CNS-residing cells is essential for cerebral endothelial activation and leukocyte recruitment. Compared with microglia, cultured astrocytes secreted a much higher level of CXCL1 in vitro. Astrocyte culture conditioned medium significantly increased the expression of VCAM-1 and ICAM-1 in cerebral endothelial cells in a CXCR2-dependent manner. Additionally, CXCR2 messenger RNA (mRNA) expression in cerebral endothelial cells but not in microglia or astrocytes was increased following tumor necrosis factor-α (TNF-α) stimulation. The intravenous injection of the CXCR2 antagonist SB225002 significantly inhibited endothelial activation and leukocyte recruitment to cerebral microvess