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Haitham Amal,1,‡ Lu Ding,2,‡ Bin-bin Liu,3,‡ Ulrike Tisch,1 Zhen-qin Xu,2 Da-you Shi,2 Yan Zhao,3 Jie Chen,3 Rui-xia Sun,3 Hu Liu,2 Sheng-Long Ye,3 Zhao-you Tang,3 Hossam Haick1 1Department of Chemical Engineering and Russell Berrie Nanotechnology Institute, Technion – Israel Institute of Technology, Haifa, Israel; 2Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, China; 3Liver Cancer Institute and Zhong-shan Hospital, Fudan University, Shanghai, China ‡These authors have equal contribution to the manuscriptBackground: Hepatocellular carcinoma (HCC) is a common and aggressive form of cancer. Due to a high rate of postoperative recurrence, the prognosis for HCC is poor. Subclinical metastasis is the major cause of tumor recurrence and patient mortality. Currently, there is no reliable prognostic method of invasion.Aim: To investigate the feasibility of fingerprints of volatile organic compounds (VOCs) for the in-vitro prediction of metastasis.Methods: Headspace gases were collected from 36 cell cultures (HCC with high and low metastatic potential and normal cells) and analyzed using nanomaterial-based sensors. Predictive models were built by employing discriminant factor analysis pattern recognition, and the classification success was determined using leave-one-out cross-validation. The chemical composition of each headspace sample was studied using gas chromatography coupled with mass spectrometry (GC-MS).Results: Excellent discrimination was achieved using the nanomaterial-based sensors between (i) all HCC and normal controls; (ii) low metastatic HCC and normal controls; (iii) high metastatic HCC and normal controls; and (iv) high and low HCC. Several HCC-related VOCs that could be associated with biochemical cellular processes were identified through GC-MS analysis.Conclusion: The presented results constitute a proof-of-concept for the in-vitro prediction of the metastat

Haitham Amal,1,‡ Lu Ding,2,‡ Bin-bin Liu,3,‡ Ulrike Tisch,1 Zhen-qin Xu,2 Da-you Shi,2 Yan Zhao,3 Jie Chen,3 Rui-xia Sun,3 Hu Liu,2 Sheng-Long Ye,3 Zhao-you Tang,3 Hossam Haick1 1Department of Chemical Engineering and Russell Berrie Nanotechnology Institute, Technion – Israel Institute of Technology, Haifa, Israel; 2Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, China; 3Liver Cancer Institute and Zhong-shan Hospital, Fudan University, Shanghai, China ‡These authors have equal contribution to the manuscriptBackground: Hepatocellular carcinoma (HCC) is a common and aggressive form of cancer. Due to a high rate of postoperative recurrence, the prognosis for HCC is poor. Subclinical metastasis is the major cause of tumor recurrence and patient mortality. Currently, there is no reliable prognostic method of invasion.Aim: To investigate the feasibility of fingerprints of volatile organic compounds (VOCs) for the in-vitro prediction of metastasis.Methods: Headspace gases were collected from 36 cell cultures (HCC with high and low metastatic potential and normal cells) and analyzed using nanomaterial-based sensors. Predictive models were built by employing discriminant factor analysis pattern recognition, and the classification success was determined using leave-one-out cross-validation. The chemical composition of each headspace sample was studied using gas chromatography coupled with mass spectrometry (GC-MS).Results: Excellent discrimination was achieved using the nanomaterial-based sensors between (i) all HCC and normal controls; (ii) low metastatic HCC and normal controls; (iii) high metastatic HCC and normal controls; and (iv) high and low HCC. Several HCC-related VOCs that could be associated with biochemical cellular processes were identified through GC-MS analysis.Conclusion: The presented results constitute a proof-of-concept for the in-vitro prediction of the metastat