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This paper investigates the fuzzy $H_{\infty}$ filter design issue for nonlinear systems with time-varying delay. In order to obtain less conservative fuzzy $H_{\infty}$ filter design method, a novel integral inequality is employed to replace the conventional Lebniz-Newton formula to analyze the stability conditions of the filtering error system. Besides, the information of the membership functions is introduced in the criterion to further relax the derived results. The proposed delay dependent filter design method is presented as LMI-based conditions, and corresponding definite expressions of fuzzy $H_{\infty}$ filter are given as well. Finally, a simulation example is provided to prove the effectiveness and superiority of the designed fuzzy $H_{\infty}$ filter., Comment: This paper was published in 2017 IEEE SMC. arXiv admin note: text overlap with arXiv:2209.04989. text overlap with arXiv:2209.04989

This paper is concerned with the fuzzy $H_{\infty}$ filter design issue for nonlinear systems with time-varying delay. To overcome the shortcomings of the conventional methods with matched preconditions, the fuzzy $H_{\infty}$ filter to be designed and the T-S fuzzy model are assumed to have different premise membership functions and number of rules, thus, greater design flexibility and robustness to uncertainty can be achieved. However, such design will also make the derived results conservative, to relax the result, a novel integral inequality which is tighter than the traditional inequalities derived from the Leibniz-Newton formula is applied, besides, a fuzzy Lypunov function and the information of the membership functions are also introduced. All the design methods are presented in LMI-based conditions. Finally, two numerical examples are given to prove the effectiveness and superiority of the proposed approach., Comment: This paper was published at IFAC 2017

© 2020, The Author(s). Large-scale, unbiased proteomics studies are constrained by the complexity of the plasma proteome. Here we report a highly parallel protein quantitation platform integrating nanoparticle (NP) protein coronas with liquid chromatography-mass spectrometry for efficient proteomic profiling. A protein corona is a protein layer adsorbed onto NPs upon contact with biofluids. Varying the physicochemical properties of engineered NPs translates to distinct protein corona patterns enabling differential and reproducible interrogation of biological samples, including deep sampling of the plasma proteome. Spike experiments confirm a linear signal response. The median coefficient of variation was 22%. We screened 43 NPs and selected a panel of 5, which detect more than 2,000 proteins from 141 plasma samples using a 96-well automated workflow in a pilot non-small cell lung cancer classification study. Our streamlined workflow combines depth of coverage and throughput with precise quantification based on unique interactions between proteins and NPs engineered for deep and scalable quantitative proteomic studies.

Qing Liu,1,2,* Hongwei Xia,2,* Sheng Zhou,1,2 Qiulin Tang,1,2 Jitao Zhou,1 Min Ren,1 Feng Bi1,2 1Department of Abdominal Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of China; 2Laboratory of Molecular Targeted Therapy in Oncology, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of China*These authors contributed equally to this workCorrespondence: Feng BiDepartment of Abdominal Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of ChinaTel +86 189 8060 1771Email bifeng@scu.edu.cnBackground: Statins, which are used to lower blood cholesterol levels by inhibiting HMG-CoA reductase, have shown anticancer effects in many cancer cells. However, the role of statins in gastric cancer remains unclear. This study aims to investigate whether the statins could antagonize progression of gastric cancer cells and tried to find the molecule mechanism.Methods: Effects of simvastatin on the morphology, proliferation, migration, apoptosis, and invasion of gastric cancer cells were detected and compared. Western blotting, cell viability assay, fluorescence, and transfection were employed to study the molecule mechanism of the effects and the interaction between YAP and β-catenin signaling.Results: Simvastatin could inhibit proliferation, migration and invasion, and promote the apoptosis in gastric cancer cells. Mechanistic studies showed that simvastatin treatment could inhibit the expression of β-catenin and the activity of YAP and the downstream targets of YAP and β-catenin in gastric cancer cells. Moreover, we found that YAP and β-catenin could form a positive feedback loop in gastric cancer cells. Further investigation revealed that simvastatin mainly acted through by inhibiting the activity of RhoA to inhibit YAP and β-catenin, and the geranylgeranyl pyrophosphate pathway media

Sheng Zhou,1,2 Meilin Ren,1,2 Huanji Xu,1,2 Hongwei Xia,2 Qiulin Tang,2 Ming Liu1 1Department of Abdominal Oncology, West China Hospital, Sichuan University, Chengdu, Sichuan Province 610041, People’s Republic of China; 2Laboratory of Molecular Targeted Therapy in Oncology, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan Province 610041, People’s Republic of ChinaCorrespondence: Qiulin Tang; Ming Liu Tel +86-28-85164045Fax +86-28-85423203Email tangqiulin1981@163.com; mingliu721@aliyun.comBackground: Colon cancer is one of the most common cancers worldwide. IFN-stimulated gene 15 (ISG15), a ubiquitin-like molecule, is strongly up-regulated by type I interferon as a crucial response to a variety of microbial and cellular stress stimuli. However, the role of ISG15 in colon cancer remains unclear.Methods: The expression of ISG15 in colon cancer tissues and cell lines was detected by using Western blotting and immunohistochemistry. ISG15 expression levels of colon cancer cells treated with trametinib was verified by using the data downloaded from the Gene Expression Omnibus (GEO) databases, quantitative real-time PCR analysis and Western blots assays. ISG15-siRNA was used to silence ISG15 in colon cancer cell line to determine the roles of ISG15 in colon cancer cell proliferation.Results: ISG15 was highly expressed in colon cancer tissues and ISG15 upregulation was closely associated with poor prognoses in colon cancer patients. Enhanced ISG15 expression promoted the migration and proliferation of colon cancer cells in vitro, while ISG15 knockdown decreased cell proliferation and metastasis. In addition, we first found that the mRNA and protein expression of ISG15 were up-regulated following trametinib treatment. Further investigation showed that ISG15 knockdown could enhance the anti-cancer effect of trametinib in colon cancer cells.Conclus

Incorporating synthetic polypeptides with intrinsic secondary structures into side chains of brush-like polymers may facilitate interesting physical properties of these materials, as the entanglement and association behaviors of the polypeptide chains significantly differ from those of flexible polymer coils. This pilot study aimed to correlate the physical properties of polypeptide-grafted brush polymers with the structure and the interactions of grafted polypeptide chains. In the 9-month project, we have synthesized a small library of brush polymers with grafted polypeptides of specific compositions and molecular weights, by integrating ring-opening metathesis polymerization (ROMP) and ring-opening polymerization of amino acid N-carboxyanhyrides (NCA). The structures and association behaviors of the grafted polypeptide chains in these brush polymers have been characterized by FTIR, NMR and X-ray scattering methods and correlated with the architecture of the macromolecules. We found that grafted polypeptides with long helical structures facilitate the intermolecular association of the brush-like polymers, and allow for the formation of robust films in which the molecular orientation can be induced by mechanical stretching or thermal annealing. The molecular nature of the entanglement behaviors found in these unique brush polymers may be further elucidated by studying the viscoelastic properties of these materials., The original document contains color images.