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Guan H, Mei Y, Mi Y, et al. Onco Targets Ther. 2018;11:4893-4899. We, the Editor and Publisher of the journal OncoTargets and Therapy have retracted the published article. Following publication of the article, concerns were raised about the duplication of images from Figure 4 with images from an unrelated article. Specifically, The images for Figure 4B, NC and si-ANRIL, have been duplicated with images for Figure 2C, NC and miR-183, 48h, respectively from Sun X, Xu Y, Zhang S, et al. (2018). MicroRNA‑183 suppresses the vitality, invasion and migration of human osteosarcoma cells by targeting metastasis‑associated protein 110.3892/etm.2021.10947. Experimental and Therapeutic Medicine. 2018;15:5058-5064. https://doi.org/10.3892/etm.2018.6068. In addition, the images for Figure 4A, NC and si-ANRIL, 0h and 4A, Blank and NC, 12h, have been duplicated. The corresponding author did not respond to our queries and was unable to provide a satisfactory explanation for how the images came to be duplicated or provide satisfactory original data for the study. As verifying the validity of published work is core to the integrity of the scholarly record, the Publisher and Editor requested to retract the article and the corresponding author was notified of this. We have been informed in our decision-making by our editorial policies and COPE guidelines. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as “Retracted”.

This paper has been retracted. Guan H, Mei Y, Mi Y, et al. Onco Targets Ther. 2018;11:4893-4899. We, the Editor and Publisher of the journal OncoTargets and Therapy have retracted the published article. Following publication of the article, concerns were raised about the duplication of images from Figure 4 with images from an unrelated article. Specifically, The images for Figure 4B, NC and si-ANRIL, have been duplicated with images for Figure 2C, NC and miR-183, 48h, respectively from Sun X, Xu Y, Zhang S, et al. (2018). MicroRNA‑183 suppresses the vitality, invasion and migration of human osteosarcoma cells by targeting metastasis‑associated protein 110.3892/etm.2021.10947. Experimental and Therapeutic Medicine. 2018;15:5058-5064. https://doi.org/10.3892/etm.2018.6068. In addition, the images for Figure 4A, NC and si-ANRIL, 0h and 4A, Blank and NC, 12h, have been duplicated. The corresponding author did not respond to our queries and was unable to provide a satisfactory explanation for how the images came to be duplicated or provide satisfactory original data for the study. As verifying the validity of published work is core to the integrity of the scholarly record, the Publisher and Editor requested to retract the article and the corresponding author was notified of this. We have been informed in our decision-making by our editorial policies and COPE guidelines. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as “Retracted”.

This study was designed to characterize two DNA replication proteins from herpes simplex virus type 1 (HSV-1): the major DNA binding protein (ICP8) and the DNA polymerase. The two proteins are among the seven proteins required for viral DNA synthesis. Four areas were investigated. First, the ICP8 protein was purified from an overproducing cell and showed to behave the same as the viral protein in interacting with DNA. To define the DNA-binding domain of the protein, a proteolytic fragment with the same DNA-binding specificity as the intact protein was identified by a protein blotting assay. N-terminal protein sequencing located the fragment between residues 300 and about 849 in the intact protein. This fragment contains some features important for DNA binding. Second, the DNA polymerase was found to replicate non-processively in vitro. The ICP8 protein slightly stimulated the polymerase activity at lower concentrations and inhibited its activity as the ICP8 concentration increased. Third, to define the functional domain of the DNA polymerase, a fragment of the polymerase predicted to contain both the polymerase and 3$\sp\prime$-5$\sp\prime$ exonuclease activities was overexpressed in Escherichia coli. The fragment was partially purified and its enzymatic activity was examined. Finally, to identify polymerase residues involved in substrate binding, three amino acid changes in polymerase mutants with altered drug sensitivities have been identified by DNA sequencing. Two mutations, affecting nucleotide binding, occur near two highly conserved regions which constitute part of a putative nucleotide binding site. This result indicates that two more residues are important for substrate binding. The third mutation, affecting pyrophosphate binding, occurs upstream of any previously known mutations. This result may indicate a new region involved in substrate binding.