1. Biochemical Characterization of Neocallimastigomycetes for the Development of Lignocellulose Conversation Technologies using Synthetic Biology
- Author
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Lankiewicz, Thomas St. Julien, O'Malley, Michelle A1, Lankiewicz, Thomas St. Julien, Lankiewicz, Thomas St. Julien, O'Malley, Michelle A1, and Lankiewicz, Thomas St. Julien
- Abstract
The global climate is in crisis, and change through disruptive technological innovation is the only option for humanity to avoid catastrophic collapses of ecosystems, economies, and, ultimately, the ability of our planet to support human life on its current magnitude. Replacing unsustainable industrial practices with novel and green processes is one area of technology that could help mitigate petroleum dependence and, by extension, damage to the biosphere. Biorefineries use synthetic biology to achieve chemical production from renewable feedstocks. Of these, lignocellulosic biorefineries that convert plant cell walls into valuable chemicals are one especially attractive concept, as lignocellulose is the most abundant renewable carbon pool in the biosphere. A central technical challenge of the lignocellulosic biorefinery concept is depolymerizing recalcitrant polymers so they can be upgraded using complementary biological and abiotic processes. Lignin is the most recalcitrant faction of plant cell walls, and this polymer is chemically distinct from the polysaccharides it protects. The aromaticity of lignin presents both a challenge and opportunity since this chemistry imparts essential properties to plant cell walls, such as hydrophobicity and resistance to degradation, that are also desirable in important consumer products like plastics. Innovation in lignin deconstruction is a current focus of lignocellulosic biorefinery research, and novel ways to degrade this polymer that are inspired by biology could help address this challenge. Large herbivores and their microbial gut communities convert lignocellulose into other chemicals at a rate and yield that eclipses our current best lignocellulosic biorefineries. My work seeks to discover the unknown aspects of biological lignin degradation as it occurs in herbivore gut microbiomes by focusing on a group of highly specialized lignocellulose degraders, The anaerobic gut fungi or Neocallimastigomycetes are some of the most
- Published
- 2022