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1. Overview of the Third International Workshop on Swine Leukocyte Differentiation Antigens

Author

Pérez de Lastra, José Manuel [0000-0003-4663-5565], Lee, Rogan [0000-0001-8364-9515], Haverson, K., Saalmüller, A., Álvarez, Belén, Alonso, Fernando, Bailey, M., Bianchi, A. T. J., Boersma, W. J. A., Chen, Zhang, Davis, William, Domínguez, Javier, Engelhardt, H., Ezquerra Martínez, Ángel, Grosmaire, L. S., Hamilton, M. J., Hollemweguer, E., Huang, C. A., Khanna, K. V., Kuebart, G., Lackovic, G., Ledbetter, J. A., Lee, Rogan, Llanes, D., Lunney, J. K., McCullough, Kenneth C., Molitor, T., Nielsen, Jens, Niewold, T. A., Pescovitz, M. D., Pérez de Lastra, José Manuel, Rehakova, Z., Salmon, H., Schnitzlein, W. M., Seebach, J., Simón, Alicia, Sinkora, J., Sinkora, M., Stokes, Christopher, Summerfield, A., Sver, L., Thacker, E., Valpotic, I., Yang, Huaizhi, Zuckermann, F., Zwart, R. J., Pérez de Lastra, José Manuel [0000-0003-4663-5565], Lee, Rogan [0000-0001-8364-9515], Haverson, K., Saalmüller, A., Álvarez, Belén, Alonso, Fernando, Bailey, M., Bianchi, A. T. J., Boersma, W. J. A., Chen, Zhang, Davis, William, Domínguez, Javier, Engelhardt, H., Ezquerra Martínez, Ángel, Grosmaire, L. S., Hamilton, M. J., Hollemweguer, E., Huang, C. A., Khanna, K. V., Kuebart, G., Lackovic, G., Ledbetter, J. A., Lee, Rogan, Llanes, D., Lunney, J. K., McCullough, Kenneth C., Molitor, T., Nielsen, Jens, Niewold, T. A., Pescovitz, M. D., Pérez de Lastra, José Manuel, Rehakova, Z., Salmon, H., Schnitzlein, W. M., Seebach, J., Simón, Alicia, Sinkora, J., Sinkora, M., Stokes, Christopher, Summerfield, A., Sver, L., Thacker, E., Valpotic, I., Yang, Huaizhi, Zuckermann, F., and Zwart, R. J.

Abstract

The aim of the Third International Workshop on Swine Leukocyte Differentiation Antigens (CD workshop), supported by the Veterinary Immunology Committee (VIC) of the International Union of Immunological Societies (IUIS), was to standardize the assignment of monoclonal antibodies (mAb) reactive with porcine leukocyte differentiation antigens and to define new antibody clusters, using nomenclature in accordance with human and ruminant CD nomenclature, as agreed at the summary meeting of the Second International Swine CD Workshop in Davis, 1995 only mAb with proven reactivity for the orthologous porcine gene product or cross-reactivity for the human gene products, were given the full CD nomenclature, all other allocations were prefixed with 'w'.As in previous workshops, the overall organization was entrusted to the chair and first author, with support by the chair of the previous workshop and second author.In addition to the existing 26 pig leukocyte CD/SWC determinants established in previous workshops, this workshop established/confirmed another 11 CDs for pig leukocytes, identified by a total of 21 mAb CD11R1 (2 mAb), CD11R2 (1 mAb), CD11R3 (4 mAb), wCD40 (1 mAb), wCD46 (4 mAb), wCD47 (3 mAb), wCD49d (1 mAb), CD61 (1 mAb), wCD92 (1 mAb), wCD93 (1 mAb) and CD163 (2 mAb). Copyright © 2001 Elsevier Science B.V.

Published
2001

2. Summary of the first round analyses of the Third International Workshop on Swine Leukocyte Differentiation Antigens

Author

Pérez de Lastra, José Manuel [0000-0003-4663-5565], Lee, Rogan [0000-0001-8364-9515], Haverson, K., Saalmüller, A., Chen, Zhang, Huang, C. A., Simón, Alicia, Seebach, J., Boersma, W. J. A., Zwart, R. J., Niewold, T. A., Thacker, E., Llanes, D., Pérez de Lastra, José Manuel, Engelhardt, H., Ezquerra Martínez, Ángel, Alonso, Fernando, Domínguez, Javier, Ledbetter, J. A., Grosmaire, L. S., Lee, Rogan, Nielsen, Jens, Salmon, H., Valpotic, I., Sver, L., Lackovic, G., Summerfield, A., Khanna, K. V., Pérez de Lastra, José Manuel [0000-0003-4663-5565], Lee, Rogan [0000-0001-8364-9515], Haverson, K., Saalmüller, A., Chen, Zhang, Huang, C. A., Simón, Alicia, Seebach, J., Boersma, W. J. A., Zwart, R. J., Niewold, T. A., Thacker, E., Llanes, D., Pérez de Lastra, José Manuel, Engelhardt, H., Ezquerra Martínez, Ángel, Alonso, Fernando, Domínguez, Javier, Ledbetter, J. A., Grosmaire, L. S., Lee, Rogan, Nielsen, Jens, Salmon, H., Valpotic, I., Sver, L., Lackovic, G., Summerfield, A., and Khanna, K. V.

Abstract

The reactivity of 155 monoclonal antibodies submitted to the Third International Workshop on Swine Leukocyte Differentiation Antigens, together with 41 internal standards, was analysed by flow cytometry on 29 different pig cell targets as well as two human cell targets as a means of establishing suitable panels of monoclonal antibodies for more detailed clustering analyses by the various subsections of the workshop. Results were collected either without further gating, with gating based on FS/SS characteristics or with gating based on the co-expression of a reference antibody in two-colour flow cytometry. The CD or SWC reactivity of the internal standards had been established in previous workshops. Data sets were subsequently analysed by statistical clustering using the Leucocyte Typing Database IV software. The resulting 18 cluster groups were allocated to the appropriate second round sections of the workshop, after reviewing the overall cellular reactivity of each cluster as well as the specificity of known standards which clustered in a group. Copyright © 2001 Elsevier Science B.V.

Published
2001

3. Summary of workshop findings for antibodies reacting with porcine T-cells and activation antigens:results from the 2. International Swine CD Workshop

Author

Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Ezquerra, A., Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Davis, W., Barbosa, I. R., Zuckermann, F. A., Saalmüller, A., Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Ezquerra, A., Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Davis, W., Barbosa, I. R., Zuckermann, F. A., and Saalmüller, A.

Published
1998

4. Summary of workshop findings for antibodies reacting with porcine T-cells and activation antigens:results from the 2. International Swine CD Workshop

Author

Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Ezquerra, A., Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Davis, W., Barbosa, I. R., Zuckermann, F. A., Saalmüller, A., Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Ezquerra, A., Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Davis, W., Barbosa, I. R., Zuckermann, F. A., and Saalmüller, A.

Published
1998

5. Summary of workshop findings for antibodies reacting with porcine T- cells and activation antigens Results from the Second International Swine CD Workshop

Author

Lee, Rogan [0000-0001-8364-9515], Pescovitz, M. D., Book, B. K., Aasted, B., Domínguez, Javier, Ezquerra Martínez, Ángel, Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, Jens, Arn, S., Sachs, David H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, Rogan, Davis, William, Barbosa, I. R., Zuckermann, F., Saalmüller, A., Lee, Rogan [0000-0001-8364-9515], Pescovitz, M. D., Book, B. K., Aasted, B., Domínguez, Javier, Ezquerra Martínez, Ángel, Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, Jens, Arn, S., Sachs, David H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, Rogan, Davis, William, Barbosa, I. R., Zuckermann, F., and Saalmüller, A.

Abstract

After initial evaluation of the 176 new and 19 control monoclonal antibodies (mAb) submitted to the Second International Swine CD Workshop, 57 were assigned to the T-cell/activation marker subgroup. These 57 mAb were further analyzed using flow cytometry on whole blood lymphocytes, splenocytes, Peyer's patch lymphocytes, in vitro cell lines, broncho-alveolar lavage cells, Con A and PHA blasts, fetal cell populations, and by 2-color flow cytometry against mAb to porcine CD2, CD4, and CD8. Finally, the molecular weights of the target antigens were characterized when possible. As a result of these analyses, 23 mAb were distributed into 7 CD clusters. Newly confirmed mAb assigments included two CD2; one CD4; two CD5; one 7 CD and one wCD25. Three new mAb were found that reacted with wCD8, one of which defined a new epitope, wCD8c. For the first time, mAb against porcine CD3 were identified, including 6 mAb that reacted with three different epitopes. Several new mAb reacted with antigens whose expression varied depending on the activation state of the test cell. These will require further characterization in order to assign a CD number.

Published
1998

6. Summary of workshop findings for antibodies reacting with porcine T-cells and activation antigens:results from the 2. International Swine CD Workshop

Author

Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Ezquerra, A., Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Davis, W., Barbosa, I. R., Zuckermann, F. A., Saalmüller, A., Pescovitz, M. D., Book, B. K., Aasted, B., Dominguez, J., Ezquerra, A., Trebichavsky, I., Novikov, B., Valpotic, I., Sver, L., Nielsen, J., Arn, S., Sachs, D. H., Lunney, J. K., Boyd, P. C., Walker, J., Lee, R., Davis, W., Barbosa, I. R., Zuckermann, F. A., and Saalmüller, A.

Published
1998

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