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Your search keyword '"Stremersch, Stephan"' showing total 4 results
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4 results on '"Stremersch, Stephan"'

1. Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering

Author

Afd Pharmaceutics, Pharmaceutics, Raes, Laurens, Pille, Melissa, Harizaj, Aranit, Goetgeluk, Glenn, van Hoeck, Jelter, Stremersch, Stephan, Fraire, Juan C, Brans, Toon, de Jong, Olivier G, Maas - Bakker, Roel, Mastrobattista, Enrico, Vader, Pieter, de Smedt, Stefaan C, Vandekerckhove, Bart, Raemdonck, Koen, Braeckmans, Kevin, Afd Pharmaceutics, Pharmaceutics, Raes, Laurens, Pille, Melissa, Harizaj, Aranit, Goetgeluk, Glenn, van Hoeck, Jelter, Stremersch, Stephan, Fraire, Juan C, Brans, Toon, de Jong, Olivier G, Maas - Bakker, Roel, Mastrobattista, Enrico, Vader, Pieter, de Smedt, Stefaan C, Vandekerckhove, Bart, Raemdonck, Koen, and Braeckmans, Kevin

Published
2021

2. Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering

Author

Afd Pharmaceutics, Pharmaceutics, Raes, Laurens, Pille, Melissa, Harizaj, Aranit, Goetgeluk, Glenn, van Hoeck, Jelter, Stremersch, Stephan, Fraire, Juan C, Brans, Toon, de Jong, Olivier G, Maas - Bakker, Roel, Mastrobattista, Enrico, Vader, Pieter, de Smedt, Stefaan C, Vandekerckhove, Bart, Raemdonck, Koen, Braeckmans, Kevin, Afd Pharmaceutics, Pharmaceutics, Raes, Laurens, Pille, Melissa, Harizaj, Aranit, Goetgeluk, Glenn, van Hoeck, Jelter, Stremersch, Stephan, Fraire, Juan C, Brans, Toon, de Jong, Olivier G, Maas - Bakker, Roel, Mastrobattista, Enrico, Vader, Pieter, de Smedt, Stefaan C, Vandekerckhove, Bart, Raemdonck, Koen, and Braeckmans, Kevin

Published
2021

3. Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering

Author

Afd Pharmaceutics, Pharmaceutics, Raes, Laurens, Pille, Melissa, Harizaj, Aranit, Goetgeluk, Glenn, van Hoeck, Jelter, Stremersch, Stephan, Fraire, Juan C, Brans, Toon, de Jong, Olivier G, Maas - Bakker, Roel, Mastrobattista, Enrico, Vader, Pieter, de Smedt, Stefaan C, Vandekerckhove, Bart, Raemdonck, Koen, Braeckmans, Kevin, Afd Pharmaceutics, Pharmaceutics, Raes, Laurens, Pille, Melissa, Harizaj, Aranit, Goetgeluk, Glenn, van Hoeck, Jelter, Stremersch, Stephan, Fraire, Juan C, Brans, Toon, de Jong, Olivier G, Maas - Bakker, Roel, Mastrobattista, Enrico, Vader, Pieter, de Smedt, Stefaan C, Vandekerckhove, Bart, Raemdonck, Koen, and Braeckmans, Kevin

Published
2021

4. On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids

Author

Deschout, Hendrik, Raemdonck, Koen, Stremersch, Stephan, Maoddi, Pietro, Mernier, Guillaume, Renaud, Philippe, Jiguet, Sébastien, Hendrix, An, Bracke, Marc, Van Den Broecke, Rudy, Röding, Magnus, Rudemo, Mats, Demeester, Jo, De Smedt, Stefaan C., Strubbe, Filip, Neyts, Kristiaan, Braeckmans, Kevin, Deschout, Hendrik, Raemdonck, Koen, Stremersch, Stephan, Maoddi, Pietro, Mernier, Guillaume, Renaud, Philippe, Jiguet, Sébastien, Hendrix, An, Bracke, Marc, Van Den Broecke, Rudy, Röding, Magnus, Rudemo, Mats, Demeester, Jo, De Smedt, Stefaan C., Strubbe, Filip, Neyts, Kristiaan, and Braeckmans, Kevin

Abstract

Cell-derived membrane vesicles that are released in biofluids, like blood or saliva, are emerging as potential non-invasive biomarkers for diseases, such as cancer. Techniques capable of measuring the size and concentration of membrane vesicles directly in biofluids are urgently needed. Fluorescence single particle tracking microscopy has the potential of doing exactly that by labelling the membrane vesicles with a fluorescent label and analysing their Brownian motion in the biofluid. However, an unbound dye in the biofluid can cause high background intensity that strongly biases the fluorescence single particle tracking size and concentration measurements. While such background intensity can be avoided with light sheet illumination, current set-ups require specialty sample holders that are not compatible with high-throughput diagnostics. Here, a microfluidic chip with integrated light sheet illumination is reported, and accurate fluorescence single particle tracking size and concentration measurements of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours are demonstrated. © 2013 The Royal Society of Chemistry.

Published
2014

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