Search

Your search keyword '"Song, Chunlin"' showing total 4 results
Start Over Author "Song, Chunlin" Publication Type Electronic Resources
4 results on '"Song, Chunlin"'

1. Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling

Author

Zhang, Yuqing, Young, Rose, Garvanska, Dimitriya H., Song, Chunlin, Zhai, Yujing, Wang, Ying, Jiang, Hongfei, Fang, Jing, Nilsson, Jakob, Alfieri, Claudio, Zhang, Gang, Zhang, Yuqing, Young, Rose, Garvanska, Dimitriya H., Song, Chunlin, Zhai, Yujing, Wang, Ying, Jiang, Hongfei, Fang, Jing, Nilsson, Jakob, Alfieri, Claudio, and Zhang, Gang

Abstract

Accurate mitosis is coordinated by the spindle assembly checkpoint (SAC) through the mitotic checkpoint complex (MCC), which inhibits the anaphase-promoting complex or cyclosome (APC/C). As an essential regulator, Cdc20 promotes mitotic exit through activating APC/C and monitors kinetochore-microtubule attachment through activating SAC. Cdc20 requires multiple interactions with APC/C and MCC subunits to elicit these functions. Functionally assessing these interactions within cells requires efficient depletion of endogenous Cdc20, which is highly difficult to achieve by RNA interference (RNAi). Here we generated Cdc20 RNAi-sensitive cell lines which display a penetrant metaphase arrest by a single RNAi treatment. In this null background, we accurately measured the contribution of each known motif of Cdc20 on APC/C and SAC activation. The CRY box, a previously identified degron, was found critical for SAC by promoting MCC formation and its interaction with APC/C. These data reveal additional regulation within the SAC and establish a novel method to interrogate Cdc20.

Published
2024

2. A robust region-adaptive digital image watermarking system

Author

Song, Chunlin

Subjects
005.82, QA75 Electronic computers. Computer science, QA76 Computer software
Abstract

Digital image watermarking techniques have drawn the attention of researchers and practitioners as a means of protecting copyright in digital images. The technique involves a subset of information-hiding technologies, which work by embedding information into a host image without perceptually altering the appearance of the host image. Despite progress in digital image watermarking technology, the main objectives of the majority of research in this area remain improvements in the imperceptibility and robustness of the watermark to attacks. Watermark attacks are often deliberately applied to a watermarked image in order to remove or destroy any watermark signals in the host data. The purpose of the attack is. aimed at disabling the copyright protection system offered by watermarking technology. Our research in the area of watermark attacks found a number of different types, which can be classified into a number of categories including removal attacks, geometry attacks, cryptographic attacks and protocol attacks. Our research also found that both pixel domain and transform domain watermarking techniques share similar levels of sensitivity to these attacks. The experiment conducted to analyse the effects of different attacks on watermarked data provided us with the conclusion that each attack affects the high and low frequency part of the watermarked image spectrum differently. Furthermore, the findings also showed that the effects of an attack can be alleviated by using a watermark image with a similar frequency spectrum to that of the host image. The results of this experiment led us to a hypothesis that would be proven by applying a watermark embedding technique which takes into account all of the above phenomena. We call this technique 'region-adaptive watermarking'. Region-adaptive watermarking is a novel embedding technique where the watermark data is embedded in different regions of the host image. The embedding algorithms use discrete wavelet transforms and a combination of discrete wavelet transforms and singular value decomposition, respectively. This technique is derived from the earlier hypothesis that the robustness of a watermarking process can be improved by using watermark data in the frequency spectrum that are not too dissimilar to that of the host data. To facilitate this, the technique utilises dual watermarking technologies and embeds parts of the watermark images into selected regions of the host image. Our experiment shows that our technique improves the robustness of the watermark data to image processing and geometric attacks, thus validating the earlier hypothesis. In addition to improving the robustness of the watermark to attacks, we can also show a novel use for the region-adaptive watermarking technique as a means of detecting whether certain types of attack have occurred. This is a unique feature of our watermarking algorithm, which separates it from other state-of-the-art techniques. The watermark detection process uses coefficients derived from the region-adaptive watermarking algorithm in a linear classifier. The experiment conducted to validate this feature shows that, on average, 94.5% of all watermark attacks can be correctly detected and identified.

Published
2012

3. Spatial separation of phosphatase and kinase activity within the Bub complex is required for proper mitosis

Author

Wang, Lei, Kruse, Thomas, López-Méndez, Blanca, Zhang, Yuqing, Song, Chunlin, Zhu, Lei, Li, Bing, Fang, Jing, Lu, Zhimin, Nilsson, Jakob, Zhang, Gang, Wang, Lei, Kruse, Thomas, López-Méndez, Blanca, Zhang, Yuqing, Song, Chunlin, Zhu, Lei, Li, Bing, Fang, Jing, Lu, Zhimin, Nilsson, Jakob, and Zhang, Gang

Abstract

The Bub1 and BubR1 kinetochore proteins support proper chromosome segregation and mitotic checkpoint activity. Bub1 and BubR1 are paralogs with Bub1 being a kinase, while BubR1 localizes the PP2A-B56 protein phosphatase to kinetochores in humans. Whether this spatial separation of kinase and phosphatase activity is important is unclear as some organisms integrate both activities into one Bub protein. Here, we engineer human Bub1 and BubR1 proteins integrating kinase and phosphatase activities into one protein and show that these do not support normal mitotic progression. A Bub1-PP2A-B56 complex can support chromosome alignment but results in impairment of the checkpoint due to dephosphorylation of the Mad1 binding site in Bub1. Furthermore, a chimeric BubR1 protein containing the Bub1 kinase domain induces delocalized H2ApT120 phosphorylation, resulting in the reduction of centromeric hSgo2 and chromosome segregation errors. Collectively, these results argue that the spatial separation of kinase and phosphatase activities within the Bub complex is required for balancing its functions in the checkpoint and chromosome alignment.

Published
2023

4. Early Prediction Model of Gestational Hypertension by Multi-Biomarkers Before 20 Weeks Gestation

Author

Zhou,Cheng, Song,Chunlin, Huang,Xiang, Chen,Shufen, Long,Yan, Zeng,Shanshui, Yang,Hongling, Jiang,Min, Zhou,Cheng, Song,Chunlin, Huang,Xiang, Chen,Shufen, Long,Yan, Zeng,Shanshui, Yang,Hongling, and Jiang,Min

Abstract

Cheng Zhou,1 Chunlin Song,1 Xiang Huang,1 Shufen Chen,1 Yan Long,2 Shanshui Zeng,2 Hongling Yang,2 Min Jiang2 1Laboratory of Molecular Diagnostics, Southern Medical University Affiliated Maternal & Child Health Hospital of Foshan, Foshan, 528000, People’s Republic of China; 2Department of Laboratory, Guangzhou Women and Children’s Medical Centre, Guangzhou Medical University, Guangzhou, 510623, People’s Republic of ChinaCorrespondence: Hongling Yang; Min JiangDepartment of Laboratory, Guangzhou Women and Children’s Medical Centre, Guangzhou Medical University, No. 9, Jinsui Road, Guangzhou, 510623, People’s Republic of ChinaTel +86-20-38857723; +86-20-38076256Email hlyang62@163.com; jiangmin2011@126.comBackground: Gestational hypertension (GH), a hypertensive disorder of pregnancy (HDP), is a leading cause of maternal and fetal mortality due to the lack of clarity on its exact etiology and clinically feasible prediction models. This study was performed to discover novel biomarkers before 20 weeks gestation and thereby construct an early GH prediction model.Methods: This study was designed based on differentially expressed protein screening followed by clinical validation. In the screening phase, a nested case-controlled study was conducted by plasma proteomic analyses using label-free LC-MS/MS and plasma samples from seven pre-GH cases before 20-week gestation and seven age- and gestational week-matched controls. In the validation phase, 10 proteins with differential expression in the screening phase were validated by ELISA or electrochemiluminescence in an independent study consisting of 29 pre-GH cases before 20-week gestation and 29 matched controls.Results: In the screening phase, 149 proteins were found to be differentially expressed between the two groups and were predominantly involved in complement and coagulation cascades, platelet degranulation and positive regulation of cell motility. Further valid

Published
2021

Catalog

Books, media, physical & digital resources
See catalog results