5 results on '"Shojaee‐Moradie, Fariba"'
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2. Fatty acid flux and oxidation are increased by rimonabant in obese women
- Author
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Backhouse, Katharine, Backhouse, Katharine, Šarac, Ivana R., Shojaee-Moradie, Fariba, Stolinski, Michael, Robertson, Denise M., Frost, Gary S., Bell, Jimmy D., Thomas, E. Louise, Wright, John, Russell-Jones, David, Umpleby, A. Margot, Backhouse, Katharine, Backhouse, Katharine, Šarac, Ivana R., Shojaee-Moradie, Fariba, Stolinski, Michael, Robertson, Denise M., Frost, Gary S., Bell, Jimmy D., Thomas, E. Louise, Wright, John, Russell-Jones, David, and Umpleby, A. Margot
- Abstract
This study aimed to determine in obese women if endocannabinoid receptor antagonism has effects on fatty acid and triglyceride metabolism and insulin sensitivity which are independent from the metabolic effects of weight loss. Fourteen obese (BMI=33.0 +/- 0.5 kg/m(2)) (mean SEM) Caucasian post-menopausal women, aged 57.8 +/- 4.7 years were studied. The women were randomised to 2 groups, one group received the endocannabinoid receptor antagonist rimonabant (20 mg/d) for 12 weeks. A control group achieved the same weight loss by a hypocaloric dietary intervention over 12 weeks. Palmitate production rate (Ra), a measure of lipolysis, and palmitate oxidation rate, and VLDL1 and VLDL2 triglyceride (TG) kinetics, were measured using isotopic tracers before and after the intervention. Weight loss was not different in the 2 groups; 2.6 +/- 0.5 kg with rimonabant and 3.1 +/- 1.0 kg in the control group. Palmitate Ra increased with rimonabant with no change in the control group (p=0.03 between groups). Palmitate oxidation rate increased with rimonabant but decreased in the control group (p=0.005 between groups). VLDL1 TG secretion rate decreased in the control group and increased in the rimonabant group (p = 0.008 between groups). There was no significant effect on insulin sensitivity. This study suggests that endocannabinoid receptor antagonism for 12 weeks in obese women increased lipolysis and fatty acid oxidation. The increase in VLDL1 TG secretion rate may be due to the increase in lipolysis which exceeded the increase in fatty acid oxidation.
- Published
- 2012
3. Gender Differences in VLDL1 and VLDL2 Triglyceride Kinetics and Fatty Acid Kinetics in Obese Postmenopausal Women and Obese Men
- Author
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Šarac, Ivana R., Šarac, Ivana R., Backhouse, Katharine, Shojaee-Moradie, Fariba, Stolinski, Michael, Robertson, Denise M., Bell, Jimmy D., Thomas, E. Louise, Hovorka, Roman, Wright, John, Umpleby, A. Margot, Šarac, Ivana R., Šarac, Ivana R., Backhouse, Katharine, Shojaee-Moradie, Fariba, Stolinski, Michael, Robertson, Denise M., Bell, Jimmy D., Thomas, E. Louise, Hovorka, Roman, Wright, John, and Umpleby, A. Margot
- Abstract
Context: High plasma triglycerides (TG) have been shown to be independent and better predictors of cardiovascular disease than low-density lipoprotein (LDL) cholesterol in women. This may be due to gender differences in very-low-density lipoprotein 1 (VLDL1)- and VLDL2-TG and fatty acid kinetics. Objective: Our objective was to investigate whether there are differences in VLDL1- and VLDL2-TG and fatty acid kinetics in obese men and postmenopausal women, a high risk group for cardiovascular disease. Research Design and Methods: Stable isotopes techniques were used to measure fasting palmitate rate of appearance, metabolic clearance rate, oxidation rate, and nonoxidative disposal rate, VLDL1-TG and VLDL2-TG fractional catabolic rate (FCR) and production rate (PR). Whole-body fat distribution was measured by magnetic resonance imaging. Participants: Participants included 10 postmenopausal obese women and eight obese men matched for age, body mass index, and fasting plasma TG. Results: The women had lower visceral fat and higher sc fat than the men (P lt 0.001 and P lt 0.002). Palmitate rate of appearance, metabolic clearance rate, nonoxidative disposal rate, and oxidation rate corrected for resting energy expenditure were greater in the women than the men (all P lt 0.03). VLDL2-TG PR corrected for fat-free mass was higher in the women (P lt 0.001). VLDL2-TG and VLDL2-cholesterol pools were higher in the women (P lt 0.001 and P lt 0.008). VLDL1-TG FCR and PR and VLDL2-TG FCR were not different between genders. Conclusion: Fatty acid and VLDL2-TG flux is higher in postmenopausal obese women than in obese men matched for fasting plasma TG levels. (J Clin Endocrinol Metab 97: 2475-2481, 2012)
- Published
- 2012
4. Gender Differences in VLDL1 and VLDL2 Triglyceride Kinetics and Fatty Acid Kinetics in Obese Postmenopausal Women and Obese Men
- Author
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Šarac, Ivana, Šarac, Ivana, Backhouse, Katharine, Shojaee-Moradie, Fariba, Stolinski, Michael, Robertson, Denise M., Bell, Jimmy D., Thomas, E. Louise, Hovorka, Roman, Wright, John, Umpleby, A. Margot, Šarac, Ivana, Šarac, Ivana, Backhouse, Katharine, Shojaee-Moradie, Fariba, Stolinski, Michael, Robertson, Denise M., Bell, Jimmy D., Thomas, E. Louise, Hovorka, Roman, Wright, John, and Umpleby, A. Margot
- Abstract
Context: High plasma triglycerides (TG) have been shown to be independent and better predictors of cardiovascular disease than low-density lipoprotein (LDL) cholesterol in women. This may be due to gender differences in very-low-density lipoprotein 1 (VLDL1)- and VLDL2-TG and fatty acid kinetics. Objective: Our objective was to investigate whether there are differences in VLDL1- and VLDL2-TG and fatty acid kinetics in obese men and postmenopausal women, a high risk group for cardiovascular disease. Research Design and Methods: Stable isotopes techniques were used to measure fasting palmitate rate of appearance, metabolic clearance rate, oxidation rate, and nonoxidative disposal rate, VLDL1-TG and VLDL2-TG fractional catabolic rate (FCR) and production rate (PR). Whole-body fat distribution was measured by magnetic resonance imaging. Participants: Participants included 10 postmenopausal obese women and eight obese men matched for age, body mass index, and fasting plasma TG. Results: The women had lower visceral fat and higher sc fat than the men (P lt 0.001 and P lt 0.002). Palmitate rate of appearance, metabolic clearance rate, nonoxidative disposal rate, and oxidation rate corrected for resting energy expenditure were greater in the women than the men (all P lt 0.03). VLDL2-TG PR corrected for fat-free mass was higher in the women (P lt 0.001). VLDL2-TG and VLDL2-cholesterol pools were higher in the women (P lt 0.001 and P lt 0.008). VLDL1-TG FCR and PR and VLDL2-TG FCR were not different between genders. Conclusion: Fatty acid and VLDL2-TG flux is higher in postmenopausal obese women than in obese men matched for fasting plasma TG levels. (J Clin Endocrinol Metab 97: 2475-2481, 2012)
- Published
- 2012
5. Investigation of total HDL and HDL subclass kinetics using stable isotope techniques in healthy subjects
- Author
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Wang, Ke, Umpleby, Margot, and Shojaee- Moradie, Fariba
- Subjects
612.1 - Abstract
Background: HDLs are heterogeneous particles, and apoA-I is the major apolipoprotein in human HDL. CVD is a multifactorial condition, and a various lipids and lipoproteins in the plasma are involved in the development of CVD. It has been suggested that HDLs have an inverse association with the risk of CVD. A high sugar intake (especially fructose and sucrose) was found to associate with a low HDL-C level and increased risk of CVD. However, the effect of dietary sugar on HDL kinetics is unclear. An insight of HDL subclass kinetics may provide a better understanding of the whole dynamic of HDL metabolism. Methods: Two studies were undertaken: 1) A controlled, randomized crossover dietary intervention study was carried out in 6 overweight middle-aged men. Subjects underwent two 12-week dietary interventions with high and low non-milk extrinsic sugar diets. Total HDL kinetics was measured using a primed constant intravenous infusion of [1-13C] leucine for 10 hours. 2) A HDL subclass kinetic study was carried out in 6 healthy subjects (3 males and 3 females). An intravenous bolus injection of [1-13C] leucine was applied to measure HDL subclass kinetics. Blood samples were taken during a 10-hour study and the following 2 weeks. Total HDL, HDL2 and HDL3 were separated from the plasma by ultracentrifugation, and αHDL and preβHDL were isolated by agarose gel electrophoresis. ApoA-I in HDL fractions was separated by SDS-PAGE. After purification, hydrolysis and derivatization, the isotopic enrichment of apoA-I in HDL was measured by GC-MS and apoA-I fractional catabolic rate (FCR) and production rate (PR) was calculated for each subclass and total HDL. Results: In the dietary intervention study, the FCR of total HDL apoA-I on the high and low sugar diet (0.20 ± 0.02 and 0.18 ± 0.02 pools/day) was similar, as was the PR (7.33 ± 0.66 and 6.05 ± 0.72 mg/kg/day respectively). In the HDL subclass study, the concentration of αHDL apoA-I (0.97 ± 0.05 g/L) was significantly higher than that of preβHDL apoA-I (0.15 ± 0.03 g/L) (p<0.001). The FCR of αHDL and preβHDL apoA-I was 0.10 ± 0.02 and 0.13 ± 0.04 pools/day, and the PR of αHDL and preβHDL apoA-I was 3.94 ± 0.73 and 0.67 ± 0.12 mg/kg/day respectively. The concentration of HDL3 apoA-I (0.68 ± 0.04 g/L) was significantly higher than that of HDL2 apoA-I (0.23 ± 0.06 g/L) (p=0.002). The FCR of HDL2 and HDL3 apoA-I was 0.15 ± 0.02 pools/day for both, and PR of HDL2 and HDL3 apoA-I was 1.35 ± 0.35 2 and 3.81 ± 0.51 mg/kg/day respectively. A significant difference was observed between αHDL and preβHDL apoA-I PR (p=0.010), and between HDL2 and HDL3 apoA-I PR (p=0.030) in the whole group. The concentration of HDL2 apoA-I was higher in women (0.32 ± 0.08 g/L) than men (0.13 ± 0.02 g/L) though the difference was not significant. HDL2 apoA-I PR was significantly higher in women than men (p=0.017). Conclusion: The high and low sugar diet did not affect HDL metabolism in overweight men. The higher apoA-I concentration of αHDL and HDL3 might be due to the higher apoA-I PR of αHDL and HDL3 compared to preβHDL and HDL2 respectively in healthy subjects. The higher level of HDL2 apoA-I in female than male subjects might be due to the higher PR of HDL2 apoA-I in women.
- Published
- 2015
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