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1. ECLAPTE: Effective Closure of LAParoTomy in Emergency-2023 World Society of Emergency Surgery guidelines for the closure of laparotomy in emergency settings

Author

Frassini, S, Cobianchi, L, Fugazzola, P, Biffl, W, Coccolini, F, Damaskos, D, Moore, E, Kluger, Y, Ceresoli, M, Coimbra, R, Davies, J, Kirkpatrick, A, Di Carlo, I, Hardcastle, T, Isik, A, Chiarugi, M, Gurusamy, K, Maier, R, Segovia Lohse, H, Jeekel, H, Boermeester, M, Abu-Zidan, F, Inaba, K, Weber, D, Augustin, G, Bonavina, L, Velmahos, G, Sartelli, M, Di Saverio, S, Ten Broek, R, Granieri, S, Dal Mas, F, Fare, C, Peverada, J, Zanghi, S, Vigano, J, Tomasoni, M, Dominioni, T, Cicuttin, E, Hecker, A, Tebala, G, Galante, J, Wani, I, Khokha, V, Sugrue, M, Scalea, T, Tan, E, Malangoni, M, Pararas, N, Podda, M, De Simone, B, Ivatury, R, Cui, Y, Kashuk, J, Peitzman, A, Kim, F, Pikoulis, E, Sganga, G, Chiara, O, Kelly, M, Marzi, I, Picetti, E, Agnoletti, V, De'Angelis, N, Campanelli, G, de Moya, M, Litvin, A, Martinez-Perez, A, Sall, I, Rizoli, S, Tomadze, G, Sakakushev, B, Stahel, P, Civil, I, Shelat, V, Costa, D, Chichom-Mefire, A, Latifi, R, Chirica, M, Amico, F, Pardhan, A, Seenarain, V, Boyapati, N, Hatz, B, Ackermann, T, Abeyasundara, S, Fenton, L, Plani, F, Sarvepalli, R, Rouhbakhshfar, O, Caleo, P, Ho-Ching Yau, V, Clement, K, Christou, E, Castillo, A, Gosal, P, Balasubramaniam, S, Hsu, J, Banphawatanarak, K, Pisano, M, Adriana, T, Michele, A, Cioffi, S, Spota, A, Catena, F, Ansaloni, L, Frassini S., Cobianchi L., Fugazzola P., Biffl W. L., Coccolini F., Damaskos D., Moore E. E., Kluger Y., Ceresoli M., Coimbra R., Davies J., Kirkpatrick A., Di Carlo I., Hardcastle T. C., Isik A., Chiarugi M., Gurusamy K., Maier R. V., Segovia Lohse H. A., Jeekel H., Boermeester M. A., Abu-Zidan F., Inaba K., Weber D. G., Augustin G., Bonavina L., Velmahos G., Sartelli M., Di Saverio S., Ten Broek R. P. G., Granieri S., Dal Mas F., Fare C. N., Peverada J., Zanghi S., Vigano J., Tomasoni M., Dominioni T., Cicuttin E., Hecker A., Tebala G. D., Galante J. M., Wani I., Khokha V., Sugrue M., Scalea T. M., Tan E., Malangoni M. A., Pararas N., Podda M., De Simone B., Ivatury R., Cui Y., Kashuk J., Peitzman A., Kim F., Pikoulis E., Sganga G., Chiara O., Kelly M. D., Marzi I., Picetti E., Agnoletti V., De'Angelis N., Campanelli G., de Moya M., Litvin A., Martinez-Perez A., Sall I., Rizoli S., Tomadze G., Sakakushev B., Stahel P. F., Civil I., Shelat V., Costa D., Chichom-Mefire A., Latifi R., Chirica M., Amico F., Pardhan A., Seenarain V., Boyapati N., Hatz B., Ackermann T., Abeyasundara S., Fenton L., Plani F., Sarvepalli R., Rouhbakhshfar O., Caleo P., Ho-Ching Yau V., Clement K., Christou E., Castillo A. M. G., Gosal P. K. S., Balasubramaniam S., Hsu J., Banphawatanarak K., Pisano M., Adriana T., Michele A., Cioffi S. P. B., Spota A., Catena F., Ansaloni L., Frassini, S, Cobianchi, L, Fugazzola, P, Biffl, W, Coccolini, F, Damaskos, D, Moore, E, Kluger, Y, Ceresoli, M, Coimbra, R, Davies, J, Kirkpatrick, A, Di Carlo, I, Hardcastle, T, Isik, A, Chiarugi, M, Gurusamy, K, Maier, R, Segovia Lohse, H, Jeekel, H, Boermeester, M, Abu-Zidan, F, Inaba, K, Weber, D, Augustin, G, Bonavina, L, Velmahos, G, Sartelli, M, Di Saverio, S, Ten Broek, R, Granieri, S, Dal Mas, F, Fare, C, Peverada, J, Zanghi, S, Vigano, J, Tomasoni, M, Dominioni, T, Cicuttin, E, Hecker, A, Tebala, G, Galante, J, Wani, I, Khokha, V, Sugrue, M, Scalea, T, Tan, E, Malangoni, M, Pararas, N, Podda, M, De Simone, B, Ivatury, R, Cui, Y, Kashuk, J, Peitzman, A, Kim, F, Pikoulis, E, Sganga, G, Chiara, O, Kelly, M, Marzi, I, Picetti, E, Agnoletti, V, De'Angelis, N, Campanelli, G, de Moya, M, Litvin, A, Martinez-Perez, A, Sall, I, Rizoli, S, Tomadze, G, Sakakushev, B, Stahel, P, Civil, I, Shelat, V, Costa, D, Chichom-Mefire, A, Latifi, R, Chirica, M, Amico, F, Pardhan, A, Seenarain, V, Boyapati, N, Hatz, B, Ackermann, T, Abeyasundara, S, Fenton, L, Plani, F, Sarvepalli, R, Rouhbakhshfar, O, Caleo, P, Ho-Ching Yau, V, Clement, K, Christou, E, Castillo, A, Gosal, P, Balasubramaniam, S, Hsu, J, Banphawatanarak, K, Pisano, M, Adriana, T, Michele, A, Cioffi, S, Spota, A, Catena, F, Ansaloni, L, Frassini S., Cobianchi L., Fugazzola P., Biffl W. L., Coccolini F., Damaskos D., Moore E. E., Kluger Y., Ceresoli M., Coimbra R., Davies J., Kirkpatrick A., Di Carlo I., Hardcastle T. C., Isik A., Chiarugi M., Gurusamy K., Maier R. V., Segovia Lohse H. A., Jeekel H., Boermeester M. A., Abu-Zidan F., Inaba K., Weber D. G., Augustin G., Bonavina L., Velmahos G., Sartelli M., Di Saverio S., Ten Broek R. P. G., Granieri S., Dal Mas F., Fare C. N., Peverada J., Zanghi S., Vigano J., Tomasoni M., Dominioni T., Cicuttin E., Hecker A., Tebala G. D., Galante J. M., Wani I., Khokha V., Sugrue M., Scalea T. M., Tan E., Malangoni M. A., Pararas N., Podda M., De Simone B., Ivatury R., Cui Y., Kashuk J., Peitzman A., Kim F., Pikoulis E., Sganga G., Chiara O., Kelly M. D., Marzi I., Picetti E., Agnoletti V., De'Angelis N., Campanelli G., de Moya M., Litvin A., Martinez-Perez A., Sall I., Rizoli S., Tomadze G., Sakakushev B., Stahel P. F., Civil I., Shelat V., Costa D., Chichom-Mefire A., Latifi R., Chirica M., Amico F., Pardhan A., Seenarain V., Boyapati N., Hatz B., Ackermann T., Abeyasundara S., Fenton L., Plani F., Sarvepalli R., Rouhbakhshfar O., Caleo P., Ho-Ching Yau V., Clement K., Christou E., Castillo A. M. G., Gosal P. K. S., Balasubramaniam S., Hsu J., Banphawatanarak K., Pisano M., Adriana T., Michele A., Cioffi S. P. B., Spota A., Catena F., and Ansaloni L.

Abstract

Laparotomy incisions provide easy and rapid access to the peritoneal cavity in case of emergency surgery. Incisional hernia (IH) is a late manifestation of the failure of abdominal wall closure and represents frequent complication of any abdominal incision: IHs can cause pain and discomfort to the patients but also clinical serious sequelae like bowel obstruction, incarceration, strangulation, and necessity of reoperation. Previous guidelines and indications in the literature consider elective settings and evidence about laparotomy closure in emergency settings is lacking. This paper aims to present the World Society of Emergency Surgery (WSES) project called ECLAPTE (Effective Closure of LAParoTomy in Emergency): the final manuscript includes guidelines on the closure of emergency laparotomy.

Published
2023

2. ECLAPTE: Effective Closure of LAParoTomy in Emergency-2023 World Society of Emergency Surgery guidelines for the closure of laparotomy in emergency settings.

Author

Frassini, S., Cobianchi, L., Fugazzola, P., Biffl, W.L., Coccolini, F., Damaskos, D., Moore, E.E., Kluger, Y., Ceresoli, M., Coimbra, R., Davies, J., Kirkpatrick, A., Carlo, I. Di, Hardcastle, T.C., Isik, A., Chiarugi, M., Gurusamy, K., Maier, R.V., Segovia Lohse, H.A., Jeekel, H., Boermeester, M.A., Abu-Zidan, F., Inaba, K., Weber, D.G., Augustin, G., Bonavina, L., Velmahos, G., Sartelli, M., Saverio, S. Di, Broek, R.P.G ten, Granieri, S., Mas, F. Dal, Farè, C.N., Peverada, J., Zanghì, S., Viganò, J., Tomasoni, M., Dominioni, T., Cicuttin, E., Hecker, A., Tebala, G.D., Galante, J.M., Wani, I., Khokha, V., Sugrue, M., Scalea, T.M., Tan, E.C.T.H., Malangoni, M.A., Pararas, N., Podda, M., Simone, B. De, Ivatury, R., Cui, Y., Kashuk, J., Peitzman, A., Kim, F., Pikoulis, E., Sganga, G., Chiara, O., Kelly, M.D., Marzi, I., Picetti, E., Agnoletti, V., De'Angelis, N., Campanelli, G., Moya, M. de, Litvin, A., Martínez-Pérez, A., Sall, I., Rizoli, S., Tomadze, G., Sakakushev, B., Stahel, P.F., Civil, I., Shelat, V., Costa, D., Chichom-Mefire, A., Latifi, R., Chirica, M., Amico, F., Pardhan, A., Seenarain, V., Boyapati, N., Hatz, B., Ackermann, T., Abeyasundara, S., Fenton, L., Plani, F., Sarvepalli, R., Rouhbakhshfar, O., Caleo, P., Ho-Ching Yau, V., Clement, K., Christou, E., Castillo, A.M.G., Gosal, P.K.S., Balasubramaniam, S., Hsu, J., Banphawatanarak, K., Pisano, M., Adriana, T., Michele, A., Cioffi, S.P.B., Spota, A., Catena, F., Ansaloni, L., Frassini, S., Cobianchi, L., Fugazzola, P., Biffl, W.L., Coccolini, F., Damaskos, D., Moore, E.E., Kluger, Y., Ceresoli, M., Coimbra, R., Davies, J., Kirkpatrick, A., Carlo, I. Di, Hardcastle, T.C., Isik, A., Chiarugi, M., Gurusamy, K., Maier, R.V., Segovia Lohse, H.A., Jeekel, H., Boermeester, M.A., Abu-Zidan, F., Inaba, K., Weber, D.G., Augustin, G., Bonavina, L., Velmahos, G., Sartelli, M., Saverio, S. Di, Broek, R.P.G ten, Granieri, S., Mas, F. Dal, Farè, C.N., Peverada, J., Zanghì, S., Viganò, J., Tomasoni, M., Dominioni, T., Cicuttin, E., Hecker, A., Tebala, G.D., Galante, J.M., Wani, I., Khokha, V., Sugrue, M., Scalea, T.M., Tan, E.C.T.H., Malangoni, M.A., Pararas, N., Podda, M., Simone, B. De, Ivatury, R., Cui, Y., Kashuk, J., Peitzman, A., Kim, F., Pikoulis, E., Sganga, G., Chiara, O., Kelly, M.D., Marzi, I., Picetti, E., Agnoletti, V., De'Angelis, N., Campanelli, G., Moya, M. de, Litvin, A., Martínez-Pérez, A., Sall, I., Rizoli, S., Tomadze, G., Sakakushev, B., Stahel, P.F., Civil, I., Shelat, V., Costa, D., Chichom-Mefire, A., Latifi, R., Chirica, M., Amico, F., Pardhan, A., Seenarain, V., Boyapati, N., Hatz, B., Ackermann, T., Abeyasundara, S., Fenton, L., Plani, F., Sarvepalli, R., Rouhbakhshfar, O., Caleo, P., Ho-Ching Yau, V., Clement, K., Christou, E., Castillo, A.M.G., Gosal, P.K.S., Balasubramaniam, S., Hsu, J., Banphawatanarak, K., Pisano, M., Adriana, T., Michele, A., Cioffi, S.P.B., Spota, A., Catena, F., and Ansaloni, L.

Abstract

Contains fulltext : 296003.pdf (Publisher’s version ) (Open Access), Laparotomy incisions provide easy and rapid access to the peritoneal cavity in case of emergency surgery. Incisional hernia (IH) is a late manifestation of the failure of abdominal wall closure and represents frequent complication of any abdominal incision: IHs can cause pain and discomfort to the patients but also clinical serious sequelae like bowel obstruction, incarceration, strangulation, and necessity of reoperation. Previous guidelines and indications in the literature consider elective settings and evidence about laparotomy closure in emergency settings is lacking. This paper aims to present the World Society of Emergency Surgery (WSES) project called ECLAPTE (Effective Closure of LAParoTomy in Emergency): the final manuscript includes guidelines on the closure of emergency laparotomy.

Published
2023

3. Evidence of Altered Guinea Pig Ventricular Cardiomyocyte Protein Expression and Growth in Response to a 5 min in vitro Exposure to H2O2

Author

Seenarain, V., Viola, H.M., Ravenscroft, G., Casey, T.M., Lipscombe, R.J., Ingley, E., Laing, N.G., Bringans, S.D., Hool, L.C., Seenarain, V., Viola, H.M., Ravenscroft, G., Casey, T.M., Lipscombe, R.J., Ingley, E., Laing, N.G., Bringans, S.D., and Hool, L.C.

Abstract

Oxidative stress and alterations in cellular calcium homeostasis are associated with the development of cardiac hypertrophy. However, the early cellular mechanisms for the development of hypertrophy are not well understood. Guinea pig ventricular myocytes were exposed to 30 μ H2O 2 for 5 min followed by 10 units/mL catalase to degrade the H 2O2, and effects on protein expression were examined 48 h later. Transient exposure to H2O2 increased the level of protein synthesis more than 2-fold, assessed as incorporation of [ 3H]leucine (n = 12; p < 0.05). Cell size was increased slightly, but there was no evidence of major cytoskeletal disorganization assessed using fluorescence microscopy. Changes in the expression of individual proteins were assessed using iTRAQ protein labeling followed by mass spectrometry analysis (LC-MALDI-MSMS); 669 proteins were identified, and transient exposure of myocytes to H2O2 altered expression of 35 proteins that were predominantly mitochondrial in origin, including TCA cycle enzymes and oxidative phosphorylation proteins. Consistent with changes in the expression of mitochondrial proteins, transient exposure of myocytes to H2O 2 increased the magnitude of the mitochondrial NADH signal 10.5 ± 2.3% compared to cells exposed to 0 μH2O2 for 5 min followed by 10 units/mL catalase (n = 8; p < 0.05). In addition, metabolic activity was significantly increased in the myocytes 48 h after transient exposure to H2O2, assessed as formation of formazan from tetrazolium salt. We conclude that a 5 min exposure of ventricular myocytes to 30 μ H2O2 is sufficient to significantly alter protein expression, consistent with the development of hypertrophy in the myocytes. Changes in mitochondrial protein expression and function appear to be early sequelae in the development of hypertrophy.

Published
2010

4. Characterisation of changes in the cardiac proteome after transient exposure of myocytes to hydrogen peroxide

Author

Seenarain, V., Viola, H., Ingley, E., Casey, T., Lipscombe, R., Hool, L., Seenarain, V., Viola, H., Ingley, E., Casey, T., Lipscombe, R., and Hool, L.

Abstract

We have shown previously that exposure of guinea pig ventricular myocytes to 30 μM hydrogen peroxide (H2O2) for 5 min followed by 10 U/ml catalase to degrade the H2O2 results in a persistent increase in superoxide production by the mitochondria. This leads to a 2-fold increase in protein synthesis measured as [3H] leucine incorporation. We characterised the effect of a transient exposure of cardiac myocytes to H2O2 on the proteome. Myocytes were exposed to 0 μM or 30 μM H2O2 for 5 min followed by 10U/ml catalase. Protein was extracted, labelled with iTRAQ reagents and the peptides were analysed by LC–MALDI-TOF/TOF mass spectrometry and identified against SwissProt and Ludwig NR databases. More than 900 proteins were identified. Transient exposure of the myocytes to H2O2 resulted in altered expression of 62 proteins. 40% of proteins identified with altered expression were mitochondrial. Consistent with our in vitro data, mitochondria complex I and complex III subunit expression was upregulated while downregulation of complex IV and ATP synthase subunits suggested compromised ATP production. We also detected an increase in sarcomere associated proteins including Troponin C, Tropomyosin I alpha isoform 6 and Myosin light chain 2 and 3. Prohibitin was downregulated and calcireticulin expression was upregulated, consistent with promotion of cellular growth. We are currently validating the findings with in vitro functional studies in the myocytes. These data suggest that transient exposure to cardiac myocytes is sufficient to significantly alter expression of proteins and provides insight into early mechanisms of development of cardiac hypertrophy.

Published
2009

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