Search

Enterococcus sp. is one of the most important causes of substantial infections worldwide. It is a fastidious micro organism with fine nutritional and environmental requirements to cultivate, a characteristic that prevents the development of useful animal models to study the biology of the micro organism. This study was designed to determine optimal conditions for culture and growth of Enterococcus sp. The bacteria Enterococcus sp. was selected from isolates of marine water. Response surface methodology was employed to optimize a bacterial biomass growth. The five variables involved in the study of growth conditions were Yeast extract, beef extract, NaCl concentration, pH and Temperature. This is an estimate of the fraction of overall variation in the data accounted by the model, and thus the model is capable of explaining 99.96% of the variation in response

Today the “Green” nanoparticle synthesis has been achieved using environmentally acceptable solvent systems and eco-friendly reducing and capping agents. The present study investigated the synthesis of silver nanoparticle using pomegranate fruit extract as reducing agent to synthesize silver nanoparticles. The formation of nanosized silver was confirmed by its characteristic surface plasmon absorption peak at around 460 nm in UV–vis spectra. The morphology and crystalline nature, were characterized by Scanning electron microscopy (SEM), energy dispersive X-ray analysis(EDAX) X-ray diffraction (XRD), and conform the active functional groups present in the synthesized silver molecule by Fourier transform infra-red (FTIR) spectroscopy. Moreover, their antibacterial activity has been tested against Bacillussubtilis, (Gram positive) and Klebsiella planticolae (Gram-negative). The approach of plant-mediated synthesis appearsto be cost efficient, eco-friendly and easy alternative to conventional methods of silver nanoparticles synthesis.

Today the “Green” nanoparticle synthesis has been achieved using environmentally acceptable solvent systems and eco-friendly reducing and capping agents. The present study investigated the synthesis of silver nanoparticle using pomegranate fruit extract as reducing agent to synthesize silver nanoparticles. The formation of nanosized silver was confirmed by its characteristic surface plasmon absorption peak at around 460 nm in UV–vis spectra. The morphology and crystalline nature, were characterized by Scanning electron microscopy (SEM), energy dispersive X-ray analysis(EDAX) X-ray diffraction (XRD), and conform the active functional groups present in the synthesized silver molecule by Fourier transform infra-red (FTIR) spectroscopy. Moreover, their antibacterial activity has been tested against Bacillussubtilis, (Gram positive) and Klebsiella planticolae (Gram-negative). The approach of plant-mediated synthesis appearsto be cost efficient, eco-friendly and easy alternative to conventional methods of silver nanoparticles synthesis.

Enterococcus sp. is one of the most important causes of substantial infections worldwide. It is a fastidious micro organism with fine nutritional and environmental requirements to cultivate, a characteristic that prevents the development of useful animal models to study the biology of the micro organism. This study was designed to determine optimal conditions for culture and growth of Enterococcus sp. The bacteria Enterococcus sp. was selected from isolates of marine water. Response surface methodology was employed to optimize a bacterial biomass growth. The five variables involved in the study of growth conditions were Yeast extract, beef extract, NaCl concentration, pH and Temperature. This is an estimate of the fraction of overall variation in the data accounted by the model, and thus the model is capable of explaining 99.96% of the variation in response