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Fatty acids have been widely used as trophic biomarkers in marine mammals. However, for the South American sea lion, the most abundant otariid in the eastern South Pacific, there is no information about blubber fatty acids and their link to diet. Here, we compare fatty acid profiles of sea lions from two distinct oceanographic regions in northern and southern Chile. Their fatty acids vary greatly between regions, suggesting dietary differences at a spatial scale. The fatty acid C22:6ω3 was more abundant in sea lions from the northern region, likely associated with consumption of anchovy, cephalopods, and crustaceans, which are rich in that fatty acid, and have been reported as their main prey items. Sea lions from the southern region were richer in C22:1 and C20:1, characteristic of teleost fish, suggesting a piscivorous diet. Males displayed a more diverse fatty acid composition than females, suggesting a wider trophic niche. Few individual sea lions within the southern region had unusually high levels of C18:2ω6, commonly found in terrestrial environments. This suggests consumption of farmed salmon, whose diet is usually based on terrestrial sources. This demonstrates how human intervention is being reflected in the tissues of a top predator in a natural environment.

Blue whales Balaenoptera musculus in the Indian Ocean (IO) are currently thought to represent 2 or 3 subspecies (B. m. intermedia, B. m. brevicauda, B. m. indica), and believed to be structured into 4 populations, each with a diagnostic song-type. Here we describe a previously unreported song-type that implies the probable existence of a population that has been undetected or conflated with another population. The novel song-type was recorded off Oman in the northern IO/Arabian Sea, off the western Chagos Archipelago in the equatorial central IO, and off Madagascar in the southwestern IO. As this is the only blue whale song that has been identified in the western Arabian Sea, we label it the ‘Northwest Indian Ocean’ song-type to distinguish it from other regional song-types. Spatiotemporal variation suggested a distribution west of 70̊ E, with potential affinity for the northern IO/Arabian Sea, and only minor presence in the southwestern IO. Timing of presence off Oman suggested that intensive illegal Soviet whaling that took 1294 blue whales in the 1960s likely targeted this population, as opposed to the more widely distributed ‘Sri Lanka’ acoustic population as previously assumed. Based upon geographic distribution and potential aseasonal reproduction found in the Soviet catch data, we suggest that if there is a northern IO subspecies (B. m. indica), it is likely this population. Moreover, the potentially restricted range, intensive historic whaling, and the fact that the song-type has been previously undetected, suggests a small population that is in critical need of status assessment and conservation action.

We test the performance of the Bayesian mixing model, MixSIAR, to quantitatively predict diets of consumers based on their fatty acids (FAs). The known diets of six species, undergoing controlled-feeding experiments, were compared with dietary predictions modelled from their FAs. Test subjects included fish, birds and mammals, and represent consumers with disparate FA compositions. We show that MixSIAR with FA data accurately identifies a consumer’s diet, the contribution of major prey items, when they change their diet (diet switching) and can detect an absent prey. Results were impacted if the consumer had a low-fat diet due to physiological constraints. Incorporating prior information on the potential prey species into the model improves model performance. Dietary predictions were reasonable even when using trophic modification values (calibration coefficients, CCs) derived from different prey. Models performed well when using CCs derived from consumers fed a varied diet or when using CC values averaged across diets. We demonstrate that MixSIAR with FAs is a powerful approach to correctly estimate diet, in particular if used to complement other methods.

Background: Most fatty acids (FAs) making up the adipose tissue in mammals have a dietary origin and suffer little modification when they are stored. However, we propose that some of those FAs, specifically those that can be synthesised or modified by mammals, are also being influenced by thermal forces and used as part of the mechanism to regulate core body temperature. As FA desaturation increases, adipose tissues can reach colder temperatures without solidifying. The ability to cool the superficial fat tissues helps create a thermal gradient, which contributes to body heat loss reduction. Therefore, it is expected that animals exposed to colder environments will possess adipose tissues with higher proportions of desaturated FAs. Here, through a model selection approach that accounts for phylogeny, we investigate how the variation in FA desaturation in 54 mammalian species relates to the thermal proxies: latitude, physical environment (terrestrial, semi-aquatic and fully-aquatic) and hair density. Results: The interaction between the environment (terrestrial, semi- or fully-aquatic) and the latitude in which the animals lived explained best the variation of FA desaturation in mammals. Aquatic mammals had higher FA desaturation compared to terrestrial mammals. Semi-aquatic mammals had significantly higher levels of desaturated FAs when living in higher latitudes whereas terrestrial and fully-aquatic mammals did not. To account for dietary influence, a double bond index was calculated including all FAs, and revealed no correlation with latitude in any of the groups. Conclusions: We propose that FA modification is an important component of the thermoregulatory strategy, particularly in semi-aquatic mammals. Potentially this is because, like terrestrial mammals, they experience the greatest air temperature variations across latitudes, but they lack a thick fur coat and rely primarily on their blubber. Unlike fully-aquatic mammals, extremely thick blubber is not ideal

Animal movement is fundamental for ecosystem functioning and species survival, yet the effects of the anthropogenic footprint on animal movements have not been estimated across species. Using a unique GPS-tracking database of 803 individuals across 57 species, we found that movements of mammals in areas with a comparatively high human footprint were on average one-half to one-third the extent of their movements in areas with a low human footprint.We attribute this reduction to behavioral changes of individual animals and to the exclusion of species with long-range movements from areas with higher human impact. Global loss of vagility alters a key ecological trait of animals that affects not only population persistence but also ecosystem processes such as predator-prey interactions, nutrient cycling, and disease transmission.

It is proposed that where sexually selected vocal communication is an honest signal, the call production rate is predicted to change throughout the breeding season. Male leopard seals call underwater for many hours each day over their three- to four-month breeding season, and it is hypothesized that a decrease in calling rate would be associated with the declining body condition of smaller males. The calling rates of leopard seals were measured (N=49 recordings) and compared between seals of different size classes throughout the breeding season. Male leopard seals produce their calls at more stable rates as they become larger. In this study, larger male leopard seals adopted a strategy of consistent underwater calling throughout the breeding season, whereas there was a breakdown in the calling stereotypy of the smaller males at its height. Toward the end of the breeding season, the smaller seals produced fewer calls in shortened calling bouts, and they took more rest periods. Therefore, underwater calling may represent an honest signal in the leopard seal. For marine mammals that call underwater, the production of repetitive sequences advertises the breath-holding ability of the caller to the listeners, and this ability may be related to male stamina and endurance, thus representing an honest signal that could be widespread in other species.

Understanding species distribution and behavior is essential for conservation programs of migratory species with recovering populations. The critically endangered Antarctic blue whale (Balaenoptera musculus intermedia) was heavily exploited during the whaling era. Because of their low numbers, highly migratory behavior, and occurrence in remote areas, their distribution and range are not fully understood, particularly in the southwest Pacific Ocean. This is the first Antarctic blue whale study covering the southwest Pacific Ocean region from temperate to tropical waters (32°S to 15°S). Passive acoustic data were recorded between 2010 and 2011 across the southwest Pacific (SWPO) and southeast Indian (SEIO) oceans. We detected Antarctic blue whale calls in previously undocumented SWPO locations off eastern Australia (32°S, 152°E) and within the Lau Basin (20°S, 176°W and 15°S, 173°W), and SEIO off northwest Australia (19°S, 115°E).In temperate waters, adjacent ocean basins had similar seasonal occurrence, in that calling Antarctic blue whales were present for long periods, almost year round in some areas. In northern tropical waters, calling whales were mostly present during the austral winter. Clarifying the occurrence and distribution of critically endangered species is fundamental for monitoring population recovery, marine protected area planning, and in mitigating anthropogenic threats.

Previous studies of the vocalization frequencies of mammals have suggested that it is either body mass or environment that drives these frequencies. Using 193 species across the globe from the terrestrial and aquatic environments and a model selection approach, we identified that the best-supported model for minimum and maximum frequencies for vocalization included both body mass and environment. The minimum frequencies of vocalizations of species from all environments retained the influence of body mass. For maximum frequency however, aquatic species are released from such a trend with body mass having little constraint on frequencies. Surprisingly, phylogeny did not have a strong impact on the evolution of the maximum frequency of mammal vocalizations, largely due to the pinniped species divergence of frequency from their carnivoran relatives. We demonstrate that the divergence of signal frequencies in mammals has arisen from the need to adapt to their environment.

Predator–prey relationships play a key role in the evolution and ecology of carnivores. An understanding of predator–prey relationships and how this differs across species and environments provides information on how carnivorous strategies have evolved and how they may change in response to environmental change. We aim to determine how mammals overcame the challenges of living within the marine environment; specifically, how this altered predator–prey body mass relationships relative to terrestrial mammals. Using predator and prey mass data collected from the literature, we applied phylogenetic piecewise regressions to investigate the relationship between predator and prey size across carnivorous mammals (51 terrestrial and 56 marine mammals). We demonstrate that carnivorous mammals have four broad dietary groups: small marine carnivores (< 11 000 kg) and small terrestrial carnivores (< 11 kg) feed on prey less than 5 kg and 2 kg, respectively. On average, large marine carnivores (> 11 000 kg) feed on prey equal to 0.01% of the carnivore's body size, compared to 45% or greater in large terrestrial carnivores (> 11 kg). We propose that differences in prey availability, and the relative ease of processing large prey in the terrestrial environment and small prey in marine environment, have led to the evolution of these novel foraging behaviours. Our results provide important insights into the selection pressures that may have been faced by early marine mammals and ultimately led to the evolution of a range of feeding strategies and predatory behaviours.

Biological markers (biomarkers) are invaluable and widely adopted in ecology, archaeology, and anthropology. Serially sampling biomarkers along continuously growing inert tissue, such as vibrissae, hair, nail, horn, or baleen, is an ideal method by which to capture the changes in an individual's diet, environment, climate, health, and stress levels. However, there are complications in calibrating the time periods that samples represent. Here, we assess how the choice of model and the input data used affect the prediction of time span. We use the Antarctic leopard seal, Hydrurga leptonyx, as our model, with stable isotope data from the vibrissae of captive and wild animals. We show that for tissues with nonlinear growth, and where fine-scale data on tissue growth are unavailable, modeling over the life span of the tissue is a simple and easily adopted approach. In this method, growth parameters are derived from surveys of the study population and the shed.replacement cycle to calibrate the time span of the biomarker data. This model performed better than linear and nonlinear models, which use parameters that have been derived from growth measurements documented over short periods relative to the life span of the tissue. These latter approaches performed in a similar fashion to each other as they tended to overestimate the life span of the tissue. Whether growth is linear or nonlinear, not accounting for position-specific differences in growth (rates or coefficients) and asymptotic length (L∞) resulted in different interpretations of biomarker data across the samples collected from the same individual. In species where metabolically inert tissue grows in a progressive fashion, the ability to account for time-specific information refines our ability to interpret the biomarker data. We recommend that this approach be adapted for tissue, such as the vibrissae and hair of mammals and the hair of humans, which exhibit the predictable growth.

We examined recordings from a 15-month (May 2009-July 2010) continuous acoustic data set collected from a bottom-mounted passive acoustic recorder at a sample frequency of 6 kHz off Portland, Victoria, Australia (38°33′01″S, 141°15′13″E) off southern Australia. Analysis revealed that calls from both subspecies were recorded at this site, and general additive modeling revealed that the number of calls varied significantly across seasons. Antarctic blue whales were detected more frequently from July to October 2009 and June to July 2010, corresponding to the suspected breeding season, while Australian blue whales were recorded more frequently from March to June 2010, coinciding with the feeding season. In both subspecies, the number of calls varied with time of day; Antarctic blue whale calls were more prevalent in the night to early morning, while Australian blue whale calls were detected more often from midday to early evening. Using passive acoustic monitoring, we show that each subspecies adopts different seasonal and daily call patterns which may be related to the ecological strategies of these subspecies. This study demonstrates the importance of passive acoustics in enabling us to understand and monitor subtle differences in the behavior and ecology of cryptic sympatric marine mammals.

For effective species management, understanding population structure and distribution is critical. However, quantifying population structure is not always straightforward. Within the Southern Hemisphere, the blue whale (Balaenoptera musculus) complex is extremely diverse but difficult to study. Using automated detector methods, we identified "acoustic populations" of whales producing region-specific call types. We examined blue whale call types in passive acoustic data at sites spanning over 7,370 km across the southeast Indian Ocean and southwest Pacific Ocean (SWPO) from 2009 to 2012. In the absence of genetic resolution, these acoustic populations offer unique information about the blue whale population complex. We found that the Australian continent acts as a geographic boundary, separating Australia and New Zealand blue whale acoustic populations at the junction of the Indian and Pacific Ocean basins. We located blue whales in previously undocumented locations, including the far SWPO, in the Tasman Sea off the east coast of Australia, and along the Lau Basin near Tonga. Our understanding of population dynamics across this broad scale has significant implications to recovery and conservation management for this endangered species, at a regional and global scale.

Predator-prey body mass relationships are a vital part of food webs across ecosystems and provide key information for predicting the susceptibility of carnivore populations to extinction. Despite this, there has been limited research on the minimum and maximum prey size of mammalian carnivores. Without information on large-scale patterns of prey mass, we limit our understanding of predation pressure, trophic cascades and susceptibility of carnivores to decreasing prey populations. The majority of studies that examine predator-prey body mass relationships focus on either a single or a subset of mammalian species, which limits the strength of our models as well as their broader application. We examine the relationship between predator body mass and the minimum, maximum and range of their prey's body mass across 108 mammalian carnivores, from weasels to baleen whales (Carnivora and Cetacea). We test whether mammals show a positive relationship between prey and predator body mass, as in reptiles and birds, as well as examine how environment (aquatic and terrestrial) and phylogenetic relatedness play a role in this relationship. We found that phylogenetic relatedness is a strong driver of predator-prey mass patterns in carnivorous mammals and accounts for a higher proportion of variance compared with the biological drivers of body mass and environment. We show a positive predator-prey body mass pattern for terrestrial mammals as found in reptiles and birds, but no relationship for aquatic mammals. Our results will benefit our understanding of trophic interactions, the susceptibility of carnivores to population declines and the role of carnivores within ecosystems. © 2014 Tucker, Rogers.

The leopard seal, Hydrurga leptonyx, is an apex predator that has a key role as consumer within the Antarctic ecosystem. Due to the constraints of traditional methods for studying feeding ecology fatty acids (FAs) analysis of blubber has become a useful tool for determining diet since it provides an indication of long-term dietary histories. FAs however, are not distributed uniformly within the blubber so understanding the degree of stratification is important in order to obtain accurate results. The FA stratification in blubber has not been examined for the leopard seal. We used whole blubber core samples from 24 individual leopard seals captured in the Danco Coast region off the Western Antarctic Peninsula (WAP). The cores were sub-sectioned into: outer, middle and inner layers and the FA compositions were compared with potential prey species (krill, fish and penguins). We found 17 FAs present at greater than trace amounts (>0.5%) across all samples. The most abundant FAs were: C18:1ω9, C16:1, C22:6ω3, C16:0 and C18:1ω7 which accounted for approximately 70% of the total. This composition of blubber is similar to other marine mammals, although some differences do exist. Principal Component Analysis confirmed clear separation between inner and outer layers where most of the FAs were present in significantly different amounts between these layers. Monounsaturated FAs (MUFA) dominated the three layers being more abundant in the outer layer. The higher presence of MUFAs, which have comparatively low melting points, in the outer layer, may suggest that this section has a thermoregulatory function. On the other hand, polyunsaturated (PUFA) and saturated FAs (SFA) were more abundant in the inner layer. PUFAs are known to be of dietary origin and SFAs are inert chemically so they can be used as long-term reserve suggesting this layer is associated with dietary intake. Moreover, the FA composition of the inner blubber layer resembles those of the prey more closely than the

After birth, mammals acquire a community of bacteria in their gastro-intestinal tract, which harvests energy and provides nutrients for the host. Comparative studies of numerous terrestrial mammal hosts have identified host phylogeny, diet and gut morphology as primary drivers of the gut bacterial community composition. To date, marine mammals have been excluded from these comparative studies, yet they represent distinct examples of evolutionary history, diet and lifestyle traits. To provide an updated understanding of the gut bacterial community of mammals, we compared bacterial 16S rRNA gene sequence data generated from faecal material of 151 marine and terrestrial mammal hosts. This included 42 hosts from a marine habitat. When compared to terrestrial mammals, marine mammals clustered separately and displayed a significantly greater average relative abundance of the phylum Fusobacteria. The marine carnivores (Antarctic and Arctic seals) and the marine herbivore (dugong) possessed significantly richer gut bacterial community than terrestrial carnivores and terrestrial herbivores, respectively. This suggests that evolutionary history and dietary items specific to the marine environment may have resulted in a gut bacterial community distinct to that identified in terrestrial mammals. Finally we hypothesize that reduced marine trophic webs, whereby marine carnivores (and herbivores) feed directly on lower trophic levels, may expose this group to high levels of secondary metabolites and influence gut microbial community richness. © 2013 Nelson et al.

Blue whales are difficult to study using traditional visual survey methods due to their endangered status and secretive behaviour. Passive acoustic monitoring (PAM) provides an alternative approach to better understand the ecology and behaviour of these rare majestic animals. PAM is cost effective, providing long term sampling that is not affected by adverse weather conditions. We compare two distinct acoustic populations of blue whales living in temperate waters across separate ocean basins. Using the CTBTO’s (Comprehensive Nuclear-test Ban Treaty Organisation) hydro-acoustic network, we have access to 15 years of continuous recordings of ocean noise from Cape Leeuwin, off Western Australia and 9 years of recordings from Juan Fernandez Island, off Chile. Hydrophones at each site record ocean noise continuously and whale calls are located using automated detectors. In addition, satellite derived environmental data was obtained which included sea surface temperature, sea surface height and productivity (chlorophyll-a). Using the satellite data, we constructed models to determine which of the environmental variables best predicted whale calls. Our results show that blue whales responded to the inter-annual variability in environmental conditions. This has implications for the management and recovery of the species as we provide some insight to how they will adapt to changing conditions.

The biochemical composition of adipose tissues plays an important role as physical barrier in mammalian thermoregulation. As fatty acid (FA) desaturation increases, adipose tissues can reach colder temperatures without solidifying. This is particularly important in bare-skinned animals and those inhabiting cold regions. We investigate how variation in FA desaturation relates to different thermal proxies. We calculate FA desaturation index for 48 mammalian species based mostly on literature values and compile a dataset of thermal proxies: Hair density, latitude and environment (terrestrial, semi-aquatic and fully-aquatic). To examine the relative contribution thermal factors play in the variation of FA desaturation, we use phylogenetic regression analyses along with a model selection approach. An interaction of environment and latitude is the model with the highest support. Adipose tissues in terrestrial mammals do not seem to have a thermoregulatory role. Fully-and semi-aquatic mammals have higher FA desaturation compared to terrestrial mammals. We show that as mammals re-invaded aquatic environments, FA modification became an important component of their thermoregulatory strategy. Semi-aquatic mammals have significantly higher levels of desaturated FAs when living in colder environments whereas terrestrial and fully-aquatic mammals do not. A high FA desaturation allows fully-aquatic mammals to maintain flexible blubber in cold environments, but surprisingly they do not modify FAs as they migrate through different latitudes. They probably regulate other blubber parameters instead. In semi-aquatic mammals, when fur is very dense the desaturation of FAs is low, and vice versa. Semi-aquatic mammals in colder environments tend to rely on blubber as an insulator.

The local warming occurring within the western Antarctic Peninsula (WAP) is causing some of the greatest environmental shifts on the planet. Over the past 140 years there have been profound biological and physical perturbations to the wildlife on the WAP. We examined whether trophic level shifts were evident in the top predators within this system and use stable isotope signatures as a proxy. Contemporary WAP leopard seals have different nitrogen isotopic values to other leopard seal populations, however historically WAP leopard seals had δ15N values within the range of other western and eastern Antarctic populations. From our 140 yr record of leopard seal tissues (n = 167), we show that the δ15N values of WAP leopard seal tissues have dropped significantly, and that this change occurs as a step around the 1980s. The magnitude (2.6o/oo δ15N) of change is ecologically significant as it reflects a drop of a trophic level within the contemporary WAP food web. Values suggest that leopard seals have shifted from eating vertebrates to krill. Over this same time period there was no shift in δ15N of the WAP krill-feeding specialist, the crabeater seal, which supports the idea that the change in leopard seal δ15N values is not due to a baseline shift in the nitrogen isotope values. Despite the century-long perturbation in the WAP, the top-predators, the seals, show a trophic downshift only in recent times, post 1980s.