1. Polypeptide effect on Mg2+ hydration inferred from CaCO3 formation: A biomineralization study by counter-diffusion
- Author
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Sancho Tomás, María, Fermani, S., Reggi, M., García Ruiz, Juan Manuel, Gómez-Morales, Jaime, Falini, G., Sancho Tomás, María, Fermani, S., Reggi, M., García Ruiz, Juan Manuel, Gómez-Morales, Jaime, and Falini, G.
- Abstract
The use of a counter-diffusion system allows the evaluation of diverse parameters involved in a crystallization process. In this study, this tool has been used to infer the hydration status of Mg during CaCO formation experiments in an agarose highly viscous sol entrapping charged polypeptides. The experimental data allow us to infer that the hydration status of Mg is altered by the presence of poly-l-aspartate or poly-l-glutamate. This changes the CaCO polymorphic distribution in favor of Mg-calcite with respect to aragonite, but does not favor the isomorphic substitution of Mg with Ca within the calcite lattice. The latter may exclude the formation of an amorphous transient form, which leads to a high Mg-calcite, as expected when using a counter-diffusion system set up. The presence of poly-l-lysine does not affect the hydration of Mg, but favors the formation of aragonite with respect to calcite. In this case an inhibition of calcite formation and an alteration of the hydration sphere of Ca could be invoked; both effects are able to increase CaCO supersaturation. In conclusion, this study reveals that charged polypeptides can orchestrate CaCO formation by also controlling the hydration status of cations.
- Published
- 2016