1. HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA) glucose ester, and linkage of pHBA into cell walls.
- Author
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Rahman, Laiq ur, Kouno, Hitomi, Hashiguchi, Yuya, Yamamoto, Hirobumi, Narbad, Arjan, Parr, Adrian, Walton, Nicholas, Ikenaga, Toshihiko, Kitamura, Yoshie, Rahman, Laiq ur, Kouno, Hitomi, Hashiguchi, Yuya, Yamamoto, Hirobumi, Narbad, Arjan, Parr, Adrian, Walton, Nicholas, Ikenaga, Toshihiko, and Kitamura, Yoshie
- Abstract
As part of a study to explore the potential for new or modified bio-product formation, Beta vulgaris (sugar beet) has been genetically modified to express in root-organ culture a bacterial gene of phenylpropanoid catabolism. The HCHL gene, encoding p-hydroxycinnamoyl-CoA hydratase/lyase, was introduced into B. vulgaris under the control of a CaMV 35S promoter, using Agrobacterium rhizogenes LBA 9402. Hairy root clones expressing the HCHL gene, together with non-expressing clones, were analysed and revealed that one expression-positive clone accumulated the glucose ester of p-hydroxybenzoic acid (pHBA) at about 14% on a dry weight basis. This is the best yield achieved in plant systems so far. Determination of cell-wall components liberated by alkaline hydrolysis confirmed that the ratio of pHBA to ferulic acid was considerably higher in the HCHL-expressing clones, whereas only ferulic acid was detected in a non-expressing clone. The change in cell-wall components also resulted in a decrease in tensile strength in the HCHL-expressing clones., Bioresource technology, 100(20), pp.4836-4842; 2009
- Published
- 2009