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Catabolic pathways for nitroaromatic compound degradation that are initiated by reductive or monooxygenase enzymes are being examined at the molecular level. The gram negative P. fluorescens strain ENV2O3O degrades p-nitrophenol oxidatively via hydroquinone. All of the genes for this catabolic pathway have been cloned and their nucleotide sequence determined. Two genes encoding for regulatory proteins have been identified. The P. fluorescents ENV2O3O cloned genes have been shown to hybridize with genomic DNA from other p-nitrophenol degrading strains (P. putida JS444 and moraxella species strain lA). The genes for p-nitrophenol degradation have been cloned from P. putida JS444. A comparison of the genes from ENV2O3O and JS444 shows that although they are 80-90% identical a gene for a key regulatory protein is missing. This explains the differences seen in how the metabolism of p-nitrophenol is regulated in the two strains. The gram negative P. pickettii strain YHlO5 degrades p-nitrobenzoate and p-aminobenzoate reductively via protocatechuate. The genes for the degradation of p-nitrobenzoate have been cloned and their nucleotide sequence determined. A regulatory gene has been identified. The gram negative strains P. syringae HYlOl and P. putida YH102 also degrade p-nitrobenzoate by a reductive route but genomic. DNA from these two strains does not cross hybridize to the genes cloned from p. pickettii YHlO5, indicating diversity in the gene sequences. The genes for p-nitrobenzoate degradation have been cloned from YHlOl and HYIO2 and shown to be very similar to each other. The cloned YH1O2 genes have been sequenced and the results show that although the HY1O2 and HY1O5 genes are 50-60% identical they are organized quite differently.

Two cases of bacteremia and one case of wound infection caused by Pseudomonas pickettii have been observed at differents moments. The bacteriological findings as well as the clinical observations are reported in the present note. Six strains have been isolated respectively in one blood culture in the first case, in three blood cultures and an infected catheter in the second case, and finally in an ankle's suppuration. All those strains exhibited the typical biochemical features of Pseudomonas pickettii. The origin of these strains, except an infected catheter, remains unknown. © 1982., SCOPUS: ar.j, info:eu-repo/semantics/published

Two cases of bacteremia and one case of wound infection caused by Pseudomonas pickettii have been observed at differents moments. The bacteriological findings as well as the clinical observations are reported in the present note. Six strains have been isolated respectively in one blood culture in the first case, in three blood cultures and an infected catheter in the second case, and finally in an ankle's suppuration. All those strains exhibited the typical biochemical features of Pseudomonas pickettii. The origin of these strains, except an infected catheter, remains unknown. © 1982., SCOPUS: ar.j, info:eu-repo/semantics/published