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Your search keyword '"Nucleic Acid Renaturation"' showing total 10 results
Start Over Descriptor "Nucleic Acid Renaturation" Publication Type Electronic Resources

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10 results on '"Nucleic Acid Renaturation"'

1. DNA sequence organization in the Thysanura Thermobia domestica.

Author

French, CK, French, CK, Manning, JE, French, CK, French, CK, and Manning, JE

Abstract

Cot and chemical analysis show that the haploid genome size of Thermobia domestica is 3-4x10(9) nucleotide pairs. Of this DNA 33% is single copy sequences and 67% is repetitive sequences. The repetitive sequences are predominantly 300 nucleotides in length and are interspersed among the single copy sequences in a short period interspersion pattern similar to that observed in Xenopus and many other higher eucaryotes. The DNA sequence organization observed in Thermobia is compared with that of other more highly evolved insects.

Published
1980

2. Complexity and content of the DNA and RNA in Trypanosoma cruzi.

Author

Lanar, DE, Lanar, DE, Levy, LS, Manning, JE, Lanar, DE, Lanar, DE, Levy, LS, and Manning, JE

Abstract

The content and sequence complexity of the nuclear DNA and messenger RNA for epimastigotes of Trypanosoma cruzi were determined. From analysis of nuclear DNA reassociation studies and microspectrofluorometric measurements of laser induced fluorescence of cellular DNA, T. cruzi is found to be a diploid organism with a nuclear DNA content of 2.5 x 10(8) nucleotide pairs (2.8 x 10(-13) g) and a kinetoplast DNA content of 4.9 x 10(7) nucleotide pairs (5.4 x 10(-14) g). Reassociation kinetics of nuclear DNA of average length 0.4 kb reveals three kinetic components: a moderately repetitive component with a reiteration frequency of 5.1 x 10(3) present in 9% of the fragments, a lowly repetitive component with a reiteration frequency of 32 present in 51% of the fragments, and a single-copy component present in 23% of the fragments. By saturation hybridization of total polysomal RNA to 3H-labeled single-copy DNA, it was determined that 68% of the single-copy DNA was represented in the epimastigote polysomal RNA. This corresponds to ca. 12 000 different mRNA species. Of these, ca. 9000 are present as poly(A)+-RNA, while the remaining 3000 appear not to be polyadenylated. Kinetic analysis of the poly(A)+-RNA population indicates it is composed of at least three classes of RNA's of different abundancy levels: two sequences which occur ca. 3000 per cell, ca. 750 sequences which occur about 20 times per cell, and ca. 15 500 sequences which occur 1-2 times per cell.

Published
1981

4. Biochemical changes during progesterone-induced maturation in Xenopus laevis oocytes

Author

Brachet, Jean, Baltus, Elyane, de Schutter, A, Hanocq, Françoise, Hanocq Quertier, Jacqueline, Hubert, E., Iacobelli, S, Steinert-Meulemans, Gilberte, Brachet, Jean, Baltus, Elyane, de Schutter, A, Hanocq, Françoise, Hanocq Quertier, Jacqueline, Hubert, E., Iacobelli, S, and Steinert-Meulemans, Gilberte

Abstract

Journal Article, SCOPUS: ar.j, info:eu-repo/semantics/published

Published
1974

6. DNA sequence organization in the Thysanura Thermobia domestica.

Author

French, CK, French, CK, Manning, JE, French, CK, French, CK, and Manning, JE

Abstract

Cot and chemical analysis show that the haploid genome size of Thermobia domestica is 3-4x10(9) nucleotide pairs. Of this DNA 33% is single copy sequences and 67% is repetitive sequences. The repetitive sequences are predominantly 300 nucleotides in length and are interspersed among the single copy sequences in a short period interspersion pattern similar to that observed in Xenopus and many other higher eucaryotes. The DNA sequence organization observed in Thermobia is compared with that of other more highly evolved insects.

Published
1980

7. Complexity and content of the DNA and RNA in Trypanosoma cruzi.

Author

Lanar, DE, Lanar, DE, Levy, LS, Manning, JE, Lanar, DE, Lanar, DE, Levy, LS, and Manning, JE

Abstract

The content and sequence complexity of the nuclear DNA and messenger RNA for epimastigotes of Trypanosoma cruzi were determined. From analysis of nuclear DNA reassociation studies and microspectrofluorometric measurements of laser induced fluorescence of cellular DNA, T. cruzi is found to be a diploid organism with a nuclear DNA content of 2.5 x 10(8) nucleotide pairs (2.8 x 10(-13) g) and a kinetoplast DNA content of 4.9 x 10(7) nucleotide pairs (5.4 x 10(-14) g). Reassociation kinetics of nuclear DNA of average length 0.4 kb reveals three kinetic components: a moderately repetitive component with a reiteration frequency of 5.1 x 10(3) present in 9% of the fragments, a lowly repetitive component with a reiteration frequency of 32 present in 51% of the fragments, and a single-copy component present in 23% of the fragments. By saturation hybridization of total polysomal RNA to 3H-labeled single-copy DNA, it was determined that 68% of the single-copy DNA was represented in the epimastigote polysomal RNA. This corresponds to ca. 12 000 different mRNA species. Of these, ca. 9000 are present as poly(A)+-RNA, while the remaining 3000 appear not to be polyadenylated. Kinetic analysis of the poly(A)+-RNA population indicates it is composed of at least three classes of RNA's of different abundancy levels: two sequences which occur ca. 3000 per cell, ca. 750 sequences which occur about 20 times per cell, and ca. 15 500 sequences which occur 1-2 times per cell.

Published
1981

9. Biochemical changes during progesterone-induced maturation in Xenopus laevis oocytes

Author

Brachet, Jean, Baltus, Elyane, de Schutter, A, Hanocq, Françoise, Hanocq Quertier, Jacqueline, Hubert, E., Iacobelli, S, Steinert-Meulemans, Gilberte, Brachet, Jean, Baltus, Elyane, de Schutter, A, Hanocq, Françoise, Hanocq Quertier, Jacqueline, Hubert, E., Iacobelli, S, and Steinert-Meulemans, Gilberte

Abstract

Journal Article, SCOPUS: ar.j, info:eu-repo/semantics/published

Published
1974

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