Search

Acute kidney injury (AKI) is considered largely reversible based on the capacity of surviving tubular cells to dedifferentiate and replace lost cells via cell division. Here we show by tracking individual tubular cells in conditional Pax8/Confetti mice that kidney function is recovered after AKI despite substantial tubular cell loss. Cell cycle and ploidy analysis upon AKI in conditional Pax8/FUCCI2aR mice and human biopsies identify endocycle-mediated hypertrophy of tubular cells. By contrast, a small subset of Pax2+ tubular progenitors enriches via higher stress resistance and clonal expansion and regenerates necrotic tubule segments, a process that can be enhanced by suitable drugs. Thus, renal functional recovery upon AKI involves remnant tubular cell hypertrophy via endocycle and limited progenitor-driven regeneration that can be pharmacologically enhanced.

The cell cycle: what it is, how it has been studied and how it will be studied The cell cycle has a pivotal role in many aspects of cell's life. The metabolic state of the cell and its decision to proliferate or even to die, as well as physiological or pathological conditions, are some of the aspects deeply influenced by the cell cycle and that, in their turn, influence it. The molecular basis of this extremely complex process has been extensively studied, nevertheless a lot of information is still missing. Indeed, old widely applied techniques as thymidine analogues incorporation have been shown to be inadequate in many current experimental settings or if employed with new scientific methodologies. Conversely, new tools recently developed, as the FUCCI technology, are a great promise and will enable to deepen our comprehension of the cell cycle by allowing scientists to study it in vivo through live imaging. With this contribution, the basis of the cell cycle and of its regulation as well as old and new techniques to study it, will be revised., non disponibile

The cell cycle: what it is, how it has been studied and how it will be studied The cell cycle has a pivotal role in many aspects of cell's life. The metabolic state of the cell and its decision to proliferate or even to die, as well as physiological or pathological conditions, are some of the aspects deeply influenced by the cell cycle and that, in their turn, influence it. The molecular basis of this extremely complex process has been extensively studied, nevertheless a lot of information is still missing. Indeed, old widely applied techniques as thymidine analogues incorporation have been shown to be inadequate in many current experimental settings or if employed with new scientific methodologies. Conversely, new tools recently developed, as the FUCCI technology, are a great promise and will enable to deepen our comprehension of the cell cycle by allowing scientists to study it in vivo through live imaging. With this contribution, the basis of the cell cycle and of its regulation as well as old and new techniques to study it, will be revised., non disponibile

The cell cycle: what it is, how it has been studied and how it will be studied The cell cycle has a pivotal role in many aspects of cell's life. The metabolic state of the cell and its decision to proliferate or even to die, as well as physiological or pathological conditions, are some of the aspects deeply influenced by the cell cycle and that, in their turn, influence it. The molecular basis of this extremely complex process has been extensively studied, nevertheless a lot of information is still missing. Indeed, old widely applied techniques as thymidine analogues incorporation have been shown to be inadequate in many current experimental settings or if employed with new scientific methodologies. Conversely, new tools recently developed, as the FUCCI technology, are a great promise and will enable to deepen our comprehension of the cell cycle by allowing scientists to study it in vivo through live imaging. With this contribution, the basis of the cell cycle and of its regulation as well as old and new techniques to study it, will be revised., non disponibile