Search

U radu su opisane reakcije aminpentacijanoferat(Il)-iona s četiri različita pralidoksima: PAM-20H, PAM-2Cl, PAM-2CH30S03, i PAM-2J. Nađena je da se spektrofotometrijskim putem mogu međusobno razlikovati pojedini oksimi zbog različite brzine re¬akcije njihovih ekvimolarnih otopina s pentacijanidnim reagensom i različite stabilnosti nastalih kompleksa (PAM-2J od ostala tri oksima), te različitih visina izmjerenih apsorbancija kod datih uvjeta eksperimenta (PAM-2 OH od ostala tri oksima). Kromatografijom pak na papiru uz upotrebu razvijača n-propanol: voda = 70:30 može se razlikovati pralidoksim-klorid (PAM-2Cl) od ostala tri ispitivana oksima zbog njegova najsporijeg putovanja. Preporučena je upotreba vodene otopine natrij-aminpentacijanoferata(II) kao »spray« reagensa za identifikaciju pralidoksima (crvene mrlje) a izvedena je i izolacija kompleksnog produkta ovih reakcija., The paper describes the reactions of aminopentacyanoferrate(II)-ions with four different pralidoximes: PAM-20H, PAM-2Cl, PAM-2CH3S03 and PAM-2J. It was found possible to distinguish spectrophotometrically between the examined oximes because of the different rates of their reaction with the pentacyanidic reagent and different stabilities of the produced complexes (PAM-21 from the other three oximes) as well as because of different absorbances measured in given experimental conditions (PAM-20H from the other three oximes). By paper chromatography, with the use of the solvent system n-propanol: water - 70:30 it was possible to distinguish pralidoxime chloride (PAM-2Cl) from the other three oximes because of its lowest rate of migration. The use of a water solution of sodium aminopentacyanoferrate(II) as »spray« reagent for the identification of pralidoximes is recommended. The complex product of these reactions has been isolated.

Stvaranje obojenog u vodi topljivog kompleksa u reakciji Toxogonina s nutrij-aminpentacijanoferatom(II) (AmPF) ili s natrij-nitrozilpentacijanoferatom(II) (NP) upotrijebljeno je za spektrofotometrijsko određivanje Toxogonina u urinu i krvnom serumu. Kod analize urina s oba reagensa može se odrediti Toxogonin u koncentracijama od 100 do 1 000 µmol/dm3 urina (ako se za analizu uzme 0,1 cm3 nativnog urina). Koeficijent varijacije kod postupka s AmPF kreće se od 2,66 do 5,31% a kod postupka s NP od 1,92 do 3,75%. Za analizu seruma može se upotrijebiti samo NP. Za područje koncentracija od 50 do 500 µmol/dm3 seruma koeficijent varijacije se kreće od 1,98 do 2,24%., Water-soluble, coloured complexes formed in the reactions of Toxogonin with either sodium aminepentacyanoferrate(II) (AmPF) or sodium nitro-sylpentacyanoferrate (II) (NP) were used for spectrophotometric determinations ot Toxogonin in urine and blood serum. Toxogonin in urine can be determined with both reagents in the concentration range from 100 to 1 OOO µmol/dm3 of urine (if analysis is performed with 0.1 cm3 of native urine). The coefficient of variation ranges from 2.66 to 5.31% in the determination with AmPF and from 1.92 to 3.75% in the determination with NP. Toxogonin in serum can be determined only by using the NP reagent. In the concentration range from 50 to 500 µmol/dm3 of serum, the coefficient of variation is between 1.98 and 2.24%.