1. Interspecies/Intergroup Complementation of Orthotospovirus Replication and Movement through Reverse Genetics Systems
- Author
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Feng, Mingfeng, Chen, Minglong, Yuan, Yulong, Liu, Qinhai, Cheng, Ruixiang, Yang, Tongqing, Li, Luyao, Guo, Rong, Dong, Yongxin, Chen, Jing, Yang, Yawen, Yan, Yuling, Cui, Hongmin, Jing, Dong, Kang, Jinrui, Chen, Shuxian, Li, Jia, Zhu, Min, Huang, Changjun, Zhang, Zhongkai, Kormelink, Richard, Tao, Xiaorong, Feng, Mingfeng, Chen, Minglong, Yuan, Yulong, Liu, Qinhai, Cheng, Ruixiang, Yang, Tongqing, Li, Luyao, Guo, Rong, Dong, Yongxin, Chen, Jing, Yang, Yawen, Yan, Yuling, Cui, Hongmin, Jing, Dong, Kang, Jinrui, Chen, Shuxian, Li, Jia, Zhu, Min, Huang, Changjun, Zhang, Zhongkai, Kormelink, Richard, and Tao, Xiaorong
- Abstract
Orthotospoviruses, the plant-infecting bunyaviruses, cause serious diseases in agronomic crops and pose major threats to global food security. The family of Tospoviridae contains more than 30 members that are classified into two geographic groups, American-type and Euro/Asian-type orthotospovirus. However, the genetic interaction between different species and the possibility, during mixed infections, for transcomplementation of gene functions by orthotospoviruses from different geographic groups remains underexplored. In this study, minireplicon-based reverse genetics (RG) systems have been established for Impatiens necrotic spot virus (INSV) (an American-type orthotospovirus) and for Calla lily chlorotic spot virus and Tomato zonate spot virus (CCSV and TZSV) (two representative Euro/Asian orthotospoviruses). Together with the earlier established RG system for Tomato spotted wilt virus (TSWV), a type species of the Orthotospovirus American-clade, viral replicase/movement proteins were exchanged and analyzed on interspecies transcomplementation. Whereas the homologous RNA-dependent RNA polymerase (RdRp) and nucleocapsid (N) protein supported the replication of orthotospoviruses from both geographic groups, heterologous combinations of RdRp from one group and N from the other group were unable to support the replication of viruses from both groups. Furthermore, the NSm movement protein (MP), from both geographic groups of orthotospoviruses, was able to transcomplement heterologous orthotospoviruses or a positive-strand Cucumber mosaic virus (CMV) in their movement, albeit with varying efficiency. MP from Rice stripe tenuivirus (RSV), a plant-infecting bunyavirus that is distinct from orthotospoviruses, or MP from CMV also moves orthotospoviruses. Our findings gain insights into the genetic interaction/reassortant potentials for the segmented plant orthotospoviruses. IMPORTANCE Orthotospoviruses are agriculturally important negative-strand RNA viruses and cause severe
- Published
- 2023