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Zhang Qing, Huang Yuan, Xiong Hao, Peng Jie Department of Radiology, Jingzhou No 1 People’s Hospital and First Affiliated Hospital of Yangtze University, Jingzhou City, Hubei Province, 434000, People’s Republic of ChinaCorrespondence: Peng Jie, Department of Radiology, The Jingzhou No 1 People’s Hospital and First Affiliated Hospital of Yangtze University, Hangkong Road, Shashi District, Jingzhou City, Hubei Province, 434000, People’s Republic of China, Tel +86 181 6313 1595, Email 18163131595vip@sina.comObjective: To explore the potential value of gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) magnetic resonance imaging (MRI) in the diagnosis of hepatocellular carcinoma (HCC) in LR-3/4 lesions by adding computed tomography (CT) delayed images based on the Liver Imaging Reporting And Data System (LI-RADS).Methods: The differences in clinical and imaging characteristics between hepatocellular carcinoma and non-HCC were compared, and logistic regression was used to analyze the imaging risk factors for the diagnosis of HCC. Based on the main and HCC-specific auxiliary features of Gd-EOB-DTPA MRI, the HCC diagnostic model 1 was established, and the diagnostic efficacy was analyzed. Based on model 1, delayed phase CT images were added to establish model 2 to find reliable predictors of HCC diagnosis. Receiver operating characteristic (ROC) analysis and the DeLong test were used to compare the two models.Results: There was a significant difference in serum AFP between HCC and non-HCC (P = 0.008). Based on main and HCC-specific auxiliary features of Gd-EOB-DTPA MRI, enhancing capsule (OR = 0.197, 95% CI = 0.06– 0.595, P = 0.005) and washout (OR = 10.345, 95% CI = 3.460– 30.930, P < 0.001) were independent risk factors in Model 1. After adding CT delayed-phase images to build model 2, enhancing capsule (OR = 0.132, 95% CI = 0.139– 0.449, P = 0.001), MRI and (or) CT washout (OR = 0.052, 95% CI = 0.016– 0.172, P < 0.001) were r

BackgroundDevelopment of neural and vascular systems displays astonishing similarities among vertebrates. This parallelism is under a precise control of complex guidance signals and neurovascular interactions. Previously, our group identified a highly conserved neural protein called thrombospondin type I domain containing 7A (THSD7A). Soluble THSD7A promoted and guided endothelial cell migration, tube formation and sprouting. In addition, we showed that thsd7a could be detected in the nervous system and was required for intersegmental vessels (ISV) patterning during zebrafish development. However, the exact origin of THSD7A and its effect on neurovascular interaction remains unclear.ResultsIn this study, we discovered that zebrafish thsd7a was expressed in the primary motor neurons. Knockdown of Thsd7a disrupted normal primary motor neuron formation and ISV sprouting in the Tg(kdr:EGFP/mnx1:TagRFP) double transgenic zebrafish. Interestingly, we found that Thsd7a morphants displayed distinct phenotypes that are very similar to the loss of Notch-delta like 4 (dll4) signaling. Transcript profiling further revealed that expression levels of notch1b and its downstream targets, vegfr2/3 and nrarpb, were down-regulated in the Thsd7a morphants. These data supported that zebrafish Thsd7a could regulate angiogenic sprouting via Notch-dll4 signaling during development.ConclusionsOur results suggested that motor neuron-derived Thsd7a plays a significant role in neurovascular interactions. Thsd7a could regulate ISV angiogenesis via Notch-dll4 signaling. Thus, Thsd7a is a potent angioneurin involved in the development of both neural and vascular systems.

A new coordination compound, [Cu(sphen)] (Hsphen = 2-sulfydryl-1,10-phenanthroline), as an analog of [Cu(ophen)] (Hophen = 1H-[1,10]phenanthrolin-2-one), is synthesized and characterized by single-crystal X-ray diffraction. While [Cu(ophen)] is a planar discrete complex showing strong π–π stacking, [Cu(sphen)] is further connected by Cu–S bonds to form a one-dimensional coordination polymer. The bulk electrical conductivity of [Cu(sphen)] (5.0 × 10−3 S·cm−1) is ~3 orders of magnitude higher than that of [Cu(ophen)] (3.9 × 10−6 S·cm−1).

A new coordination compound, [Cu(sphen)] (Hsphen = 2-sulfydryl-1,10-phenanthroline), as an analog of [Cu(ophen)] (Hophen = 1H-[1,10]phenanthrolin-2-one), is synthesized and characterized by single-crystal X-ray diffraction. While [Cu(ophen)] is a planar discrete complex showing strong π–π stacking, [Cu(sphen)] is further connected by Cu–S bonds to form a one-dimensional coordination polymer. The bulk electrical conductivity of [Cu(sphen)] (5.0 × 10−3 S·cm−1) is ~3 orders of magnitude higher than that of [Cu(ophen)] (3.9 × 10−6 S·cm−1).

BackgroundDevelopment of neural and vascular systems displays astonishing similarities among vertebrates. This parallelism is under a precise control of complex guidance signals and neurovascular interactions. Previously, our group identified a highly conserved neural protein called thrombospondin type I domain containing 7A (THSD7A). Soluble THSD7A promoted and guided endothelial cell migration, tube formation and sprouting. In addition, we showed that thsd7a could be detected in the nervous system and was required for intersegmental vessels (ISV) patterning during zebrafish development. However, the exact origin of THSD7A and its effect on neurovascular interaction remains unclear.ResultsIn this study, we discovered that zebrafish thsd7a was expressed in the primary motor neurons. Knockdown of Thsd7a disrupted normal primary motor neuron formation and ISV sprouting in the Tg(kdr:EGFP/mnx1:TagRFP) double transgenic zebrafish. Interestingly, we found that Thsd7a morphants displayed distinct phenotypes that are very similar to the loss of Notch-delta like 4 (dll4) signaling. Transcript profiling further revealed that expression levels of notch1b and its downstream targets, vegfr2/3 and nrarpb, were down-regulated in the Thsd7a morphants. These data supported that zebrafish Thsd7a could regulate angiogenic sprouting via Notch-dll4 signaling during development.ConclusionsOur results suggested that motor neuron-derived Thsd7a plays a significant role in neurovascular interactions. Thsd7a could regulate ISV angiogenesis via Notch-dll4 signaling. Thus, Thsd7a is a potent angioneurin involved in the development of both neural and vascular systems.