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Previous genome-scale phylogenetic analyses of Fungi have under sampled taxa from Zoopagales; this order contains many predacious or parasitic genera, and most have never been grown in pure culture. We sequenced the genomes of 4 zoopagalean taxa that are predators of amoebae, nematodes, or rotifers and the genome of one taxon that is a parasite of amoebae using single cell sequencing methods with whole genome amplification. Each genome was a metagenome, which was assembled and binned using multiple techniques to identify the target genomes. We inferred phylogenies with both super matrix and coalescent approaches using 192 conserved proteins mined from the target genomes and performed ancestral state reconstructions to determine the ancestral trophic lifestyle of the clade. Our results indicate that Zoopagales is monophyletic. Ancestral state reconstructions provide moderate support for mycoparasitism being the ancestral state of the clade.

The purpose of this document is to report a needs assessment questionnaire package based on the position that needs assessment surveys should gather the preferences of prospective participants regarding all modifiable aspects of an inservice program. The package consists of two instruments that may be administered either separately or in combination. The first instrument, the Experience-Interest Inventory, illustrates a form for assessing prospective participants' relative interests and levels of experience regarding a number of program topics. The second instrument, the Design for Staff Development Preference Inventory, asks respondents about areas relevant to the motivational and structural design of the intended inservice program. Each instrument was administered to over five hundred principals of urban and Title I schools across the nation. A preliminary analysis of results suggests that administrative inservice programs fashioned after the preferences of this national sample of principals tend to exhibit higher levels of participant satisfaction with the program than do programs not so designed. The rationale upon which the instruments are posited is discussed. Subsequent sections of the report present the instruments themselves and list computer programs that can be used to score the instruments. The report concludes with a brief description of a "model" inservice program drawn solely from data obtained through application of the questionnaires. (Author/JD)