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Following the pandemic, outpatient waiting lists within Wales have risen significantly (Welsh Government, 2023a). Patients are waiting longer than ever before to receive treatment (Welsh Government, 2023a). To tackle waiting list (WL) issues, one solution proposed by Cardiff and Vale University Health Board (C&VUHB) is Patient Participation Booking (PPB). Evaluation will occur on how successful PPB is in achieving its aim of decreasing the Did Not Attend (DNA) rate for new acute outpatient appointments (NAOA) within C&VUHB. PPB’s success in lowering the demand on administrative services will also be a particular focus, along with improving patient communication.

In light of recently found deviations of the experimental data from predictions from QCD factorization for $B_{(s)}\rightarrow D_{(s)}P$ decays, where $P=\{\pi,K\}$, we systematically probe the current status of the SU(3)$_F$ expansion from a fit to experimental branching ratio data without any further theory input. We find that the current data is in agreement with the power-counting of the SU(3)$_F$ expansion. While the SU(3)$_F$ limit is excluded at $>5\sigma$, SU(3)$_F$-breaking contributions of $\sim 20\%$ suffice for an excellent description of the data. SU(3)$_F$ breaking is needed in tree ($>5\sigma$) and color-suppressed tree ($2.4\sigma$) diagrams. We are not yet sensitive to SU(3)$_F$ breaking in exchange diagrams. From the underlying SU(3)$_F$ parametrization we predict the unmeasured branching ratios $\mathcal{B}(\overline{B}_s^0\rightarrow \pi^- D^+) = 2 \mathcal{B}(\overline{B}_s^0\rightarrow \pi^0 D^0) = [0.3, 7.2] \times 10^{-6}$ of suppressed decays that can be searched for at the LHCb Upgrade I and LHCb Upgrade II experiments respectively., Comment: 7 pages

8-oxo Guanine DNA Glycosylase 1 is the initiating enzyme within base excision repair and removes oxidized guanines from damaged DNA. Since unrepaired 8-oxoG could lead to G : C→T : A transversion, base removal is of utmost importance for cells to ensure genomic integrity. For cells with elevated levels of reactive oxygen species this dependency is further increased. In the past we and others have validated OGG1 as a target for inhibitors to treat cancer and inflammation. Here, we present the optimization campaign that led to the broadly used tool compound TH5487. Based on results from a small molecule screening campaign, we performed hit to lead expansion and arrived at potent and selective substituted N-piperidinyl-benzimidazolones. Using X-ray crystallography data, we describe the surprising binding mode of the most potent member of the class, TH8535. Here, the N-Piperidinyl-linker adopts a chair instead of a boat conformation which was found for weaker analogues. We further demonstrate cellular target engagement and efficacy of TH8535 against a number of cancer cell lines.

We perform a detailed model-independent phenomenological analysis of $\bar B\to \bar D D$ decays. Employing an SU(3)$_F$ analysis including symmetry-breaking contributions together with a conservative power counting for various suppression effects, we obtain updated Standard Model predictions for all branching fractions and CP asymmetries from a fit to the available experimental data, testable at Belle II and the LHC experiments. These results include all relevant suppressed contributions, thereby providing upper limits on subleading Standard Model (SM) effects like "penguin pollution", enabling searches for physics beyond the SM. Importantly, allowing in the same fit for the production fractions of charged and neutral $B$ mesons to be different, we find $f_d/f_u = 0.86\pm0.05$, which is $2.5\sigma$ away from unity, which, if confirmed, would have important phenomenological consequences., Comment: 24 pages. Matches published version

The fates of massive galaxies are tied to the evolution of their central supermassive black holes (BHs), due to the influence of AGN feedback. Correlations within simulated galaxy populations suggest that the masses of BHs are governed by properties of their host dark matter haloes, such as the binding energy and assembly time, at a given halo mass. However, the full picture must be more complex as galaxy mergers have also been shown to influence the growth of BHs and the impact of AGN. In this study, we investigate this problem through a controlled experiment, using the genetic modification technique to adjust the assembly history of a Milky Way-like galaxy simulated with the EAGLE model. We change the halo assembly time (and hence the binding energy) in the absence of any disruptive merger events, and find little change in the integrated growth of the BH. We attribute this to the angular momentum support provided by a galaxy disc, which reduces the inflow of gas towards the BH and effectively decouples the BH's growth from the halo's properties. Introducing major mergers into the assembly history disrupts the disc, causing the BH to grow $\approx 4\times$ more massive and inject feedback that reduces the halo baryon fraction by a factor of $\approx 2$ and quenches star formation. Merger events appear essential to the diversity in BH masses in EAGLE, and we also show that they increase the halo binding energy; correlations between these quantities may therefore be the result of merger events., Comment: 12 pages, 5 figures. Accepted for publication in MNRAS

We use zoom simulations to show how merger-driven disruption of the gas disc in a galaxy provides its central active galactic nucleus (AGN) with fuel to drive outflows that entrain and expel a significant fraction of the circumgalactic medium (CGM). This in turn suppresses replenishment of the interstellar medium, causing the galaxy to quench up to several Gyr after the merger. We start by performing a zoom simulation of a present-day star-forming disc galaxy with the EAGLE galaxy formation model. Then, we re-simulate the galaxy with controlled changes to its initial conditions, using the genetic modification technique. These modifications either increase or decrease the stellar mass ratio of the galaxy's last significant merger, which occurs at $z\approx 0.74$. The halo reaches the same present-day mass in all cases, but changing the mass ratio of the merger yields markedly different galaxy and CGM properties. We find that a merger can unlock rapid growth of the central supermassive black hole if it disrupts the co-rotational motion of gas in the black hole's vicinity. Conversely, if a less disruptive merger occurs and gas close to the black hole is not disturbed, the AGN does not strongly affect the CGM, and consequently the galaxy continues to form stars. Our result illustrates how a unified view of AGN feedback, the baryon cycle and the interstellar medium is required to understand how mergers and quenching are connected over long timescales., Comment: 13 pages, 8 figures. Submitted to MNRAS, comments welcome

Oxidative DNA damage is recognized by 8-oxoguanine (8-oxoG) DNA glycosylase 1 (OGG1), which excises 8-oxoG, leaving a substrate for apurinic endonuclease 1 (APE1) and initiating repair. Here, we describe a small molecule (TH10785) that interacts with the phenylalanine-319 and glycine-42 amino acids of OGG1, increases the enzyme activity 10-fold, and generates a previously undescribed beta,delta-lyase enzymatic function. TH10785 controls the catalytic activity mediated by a nitrogen base within its molecular structure. In cells, TH10785 increases OGG1 recruitment to and repair of oxidative DNA damage. This alters the repair process, which no longer requires APE1 but instead is dependent on polynucleotide kinase phosphatase (PNKP1) activity. The increased repair of oxidative DNA lesions with a small molecule may have therapeutic applications in various diseases and aging.

Botulinum neurotoxins (BoNTs) are the causative agents of a potentially lethal paralytic disease targeting cholinergic nerve terminals. Multiple BoNT serotypes exist, with types A, B and E being the main cause of human botulism. Their extreme toxicity has been exploited for cosmetic and therapeutic uses to treat a wide range of neuromuscular disorders. Although naturally occurring BoNT types share a common end effect, their activity varies significantly based on the neuronal cell-surface receptors and intracellular SNARE substrates they target. These properties are the result of structural variations that have traditionally been studied using biophysical methods such as X-ray crystallography. Here, we determined the first structures of botulinum neurotoxins using single-particle cryogenic electron microscopy. The maps obtained at 3.6 and 3.7 Å for BoNT/B and /E, respectively, highlight the subtle structural dynamism between domains, and of the binding domain in particular. This study demonstrates how the recent advances made in the field of single-particle electron microscopy can be applied to bacterial toxins of clinical relevance and the botulinum neurotoxin family in particular.

The challenging task of embedding innovative participatory processes and technologies within local government often falls upon local council officers. Using qualitative data collection and analysis, we investigate the ongoing work of Scottish local councils seeking to run the process of participatory budgeting (PB) within their institution, the use of digital platforms to support this and the challenges faced. In doing so this paper draws on empirical material to support the growing discussion on the dynamics or forces behind embedding. Our analysis shows that formal agreement alone does not make the process a certainty. Local council officers must work as mediators in the transitional space between representative structures and new, innovative ways of working, unsettling the entrenched power dynamics. To do so they must be well trained and well resourced, including the ability to use digital platforms effectively as part of the process. This provides the necessary, accessible, transparent and deliberative space for participation., Comment: 12 pages, presented at the 15th International Conference on Theory and Practice of Electronic Governance 2022

Alzheimer's disease (AD) is a chronic neurodegenerative disease characterized by the progressive accumulation of amyloid-beta and neurofibrillary tangles of tau in the neocortex. We profiled DNA methylation in two regions of the cortex from 631 donors, performing an epigenome-wide association study of multiple measures of AD neuropathology. We meta-analyzed our results with those from previous studies of DNA methylation in AD cortex (total n = 2013 donors), identifying 334 cortical differentially methylated positions (DMPs) associated with AD pathology including methylomic variation at loci not previously implicated in dementia. We subsequently profiled DNA methylation in NeuN+ (neuronal-enriched), SOX10+ (oligodendrocyte-enriched) and NeuN-/SOX10- (microglia- and astrocyte-enriched) nuclei, finding that the majority of DMPs identified in 'bulk' cortex tissue reflect DNA methylation differences occurring in non-neuronal cells. Our study highlights the power of utilizing multiple measures of neuropathology to identify epigenetic signatures of AD and the importance of characterizing disease-associated variation in purified cell-types.

We use archival Herschel data to examine the singly ionized carbon ([CII]) content of 14 star forming dwarf galaxies in the Virgo cluster. We use spectral energy distribution (SED) fits to far infrared, mid infrared, near infrared, optical and ultraviolet data to derive the total infrared continuum (TIR) for these galaxies. We compare the [CII]/TIR ratio for dwarf galaxies in the central part of Virgo to those in the southern part of the cluster and to galaxies with similar TIR luminosities and metallicities in the Herschel Dwarf Galaxy Survey (DGS) sample of field dwarf galaxies to look for signs of [CII] formation independent of star formation. Our analysis indicates that the sample of Virgo dwarfs in the central part of the cluster has significantly higher values of [CII]/TIR than the sample from the southern part of the cluster and the sample from the DGS, while the southern sample is consistent with the DGS. This [CII]/TIR excess implies that a significant fraction of the [CII] in the dwarf galaxies in the cluster center has an origin other than star formation and is likely to be due to environmental processes in the central part of the cluster. We also find a surprisingly strong correlation between [CII]/TIR and the local ram pressure felt by the dwarf galaxies in the cluster. In this respect, we claim that the excess [CII] we see in these galaxies is likely to be due to formation in ram pressure shocks., Comment: 20 pages, accepted for publication in the Astronomical Journal

We investigate how the X-ray circumgalactic medium (CGM) of present-day galaxies depends on galaxy morphology and azimuthal angle using mock observations generated from the EAGLE cosmological hydrodynamic simulation. By creating mock stacks of {\it eROSITA}-observed galaxies oriented to be edge-on, we make several observationally-testable predictions for galaxies in the stellar mass range $M_\star=10^{10.7-11.2}\;$M$_{\odot}$. The soft X-ray CGM of disc galaxies is between 60 and 100\% brighter along the semi-major axis compared to the semi-minor axis, between 10-30 kpc. This azimuthal dependence is a consequence of the hot ($T>10^6$ K) CGM being non-spherical: specifically it is flattened along the minor axis such that denser and more luminous gas resides in the disc plane and co-rotates with the galaxy. Outflows enrich and heat the CGM preferentially perpendicular to the disc, but we do not find an observationally-detectable signature along the semi-minor axis. Spheroidal galaxies have hotter CGMs than disc galaxies related to spheroids residing at higher halos masses, which may be measurable through hardness ratios spanning the $0.2-1.5$ keV band. While spheroids appear to have brighter CGMs than discs for the selected fixed $M_\star$ bin, this owes to spheroids having higher stellar and halo masses within that $M_\star$ bin, and obscures the fact that both simulated populations have similar total CGM luminosities at the exact same $M_\star$. Discs have brighter emission inside 20 kpc and more steeply declining profiles with radius than spheroids. We predict that the {\it eROSITA} 4-year all-sky survey should detect many of the signatures we predict here, although targeted follow-up observations of highly inclined nearby discs after the survey may be necessary to observe some of our azimuthally-dependent predictions., Comment: 12 pages, 11 figures, 1 table. Submitted to MNRAS. Comments welcome

Throughout spring and summer 2020, ozone stations in the northern extratropics recorded unusually low ozone in the free troposphere. From April to August, and from 1 to 8 kilometers altitude, ozone was on average 7% (≈4 nmol/mol) below the 2000 to 2020 climatological mean. Such low ozone, over several months, and at so many stations, has not been observed in any previous year since at least 2000. Atmospheric composition analyses from the Copernicus Atmosphere Monitoring Service and simulations from the NASA GMI model indicate that the large 2020 springtime ozone depletion in the Arctic stratosphere contributed less than one quarter of the observed tropospheric anomaly. The observed anomaly is consistent with recent chemistry-climate model simulations, which assume emissions reductions similar to those caused by the COVID-19 crisis. COVID-19 related emissions reductions appear to be the major cause for the observed reduced free tropospheric ozone in 2020.

The botulinum neurotoxins are potent molecules that are not only responsible for the lethal paralytic disease botulism, but have also been harnessed for therapeutic uses in the treatment of an increasing number of chronic neurological and neuromuscular disorders, in addition to cosmetic applications. The toxins act at the cholinergic nerve terminals thanks to an efficient and specific mechanism of cell recognition which is based on a dual receptor system that involves gangliosides and protein receptors. Binding to surface-anchored gangliosides is the first essential step in this process. Here, we determined the X-ray crystal structure of the binding domain of BoNT/E, a toxin of clinical interest, in complex with its GD1a oligosaccharide receptor. Beyond confirmation of the conserved ganglioside binding site, we identified key interacting residues that are unique to BoNT/E and a significant rearrangement of loop 1228-1237 upon carbohydrate binding. These observations were also supported by thermodynamic measurements of the binding reaction and assessment of ganglioside selectivity by immobilised-receptor binding assays. These results provide a structural basis to understand the specificity of BoNT/E for complex gangliosides.

The enzyme NUDT15 efficiently hydrolyzes the active metabolites of thiopurine drugs, which are routinely used for treating cancer and inflammatory diseases. Loss-of-function variants in NUDT15 are strongly associated with thiopurine intolerance, such as leukopenia, and preemptive NUDT15 genotyping has been clinically implemented to personalize thiopurine dosing. However, understanding the molecular consequences of these variants has been difficult, as no structural information was available for NUDT15 proteins encoded by clinically actionable pharmacogenetic variants because of their inherent instability. Recently, the small molecule NUDT15 inhibitor TH1760 has been shown to sensitize cells to thiopurines, through enhanced accumulation of 6-thio-guanine in DNA. Building upon this, we herein report the development of the potent and specific NUDT15 inhibitor, TH7755. TH7755 demonstrates a greatly improved cellular target engagement and 6-thioguanine potentiation compared with TH1760, while showing no cytotoxicity on its own. This potent inhibitor also stabilized NUDT15, enabling analysis by X-ray crystallography. We have determined high-resolution structures of the clinically relevant NUDT15 variants Arg139Cys, Arg139His, Val18Ile, and V18_V19insGlyVal. These structures provide clear insights into the structural basis for the thiopurine intolerance phenotype observed in patients carrying these pharmacogenetic variants. These findings will aid in predicting the effects of new NUDT15 sequence variations yet to be discovered in the clinic.

In recent years participatory budgeting (PB) in Scotland has grown from a handful of community-led processes to a movement supported by local and national government. This is epitomized by an agreement between the Scottish Government and the Convention of Scottish Local Authorities (COSLA) that at least 1% of local authority budgets will be subject to PB. This ongoing research paper explores the challenges that emerge from this 'scaling up' or 'mainstreaming' across the 32 local authorities that make up Scotland. The main objective is to evaluate local authority use of the digital platform Consul, which applies Natural Language Processing (NLP) to address these challenges. This project adopts a qualitative longitudinal design with interviews, observations of PB processes, and analysis of the digital platform data. Thematic analysis is employed to capture the major issues and themes which emerge. Longitudinal analysis then explores how these evolve over time. The potential for 32 live study sites provides a unique opportunity to explore discrete political and social contexts which materialize and allow for a deeper dive into the challenges and issues that may exist, something a wider cross-sectional study would miss. Initial results show that issues and challenges which come from scaling up may be tackled using NLP technology which, in a previous controlled use case-based evaluation, has shown to improve the effectiveness of citizen participation., Comment: 7 pages, presented at the 14th International Conference on Theory and Practice of Electronic Governance 2021. arXiv admin note: text overlap with arXiv:2109.09517

© 2020 American Physical Society. In order to perform Bayesian parameter estimation to infer the source properties of gravitational waves from compact binary coalescences (CBCs), the noise characteristics of the detector must be understood. It is typically assumed that the detector noise is stationary and Gaussian, characterized by a power spectral density (PSD) that is measured with infinite precision. We present a new method to incorporate the uncertainty in the power spectral density estimation into the Bayesian inference of the binary source parameters and apply it to the first 11 CBC detections reported by the LIGO-Virgo Collaboration. We find that incorporating the PSD uncertainty only leads to variations in the positions and widths of the binary parameter posteriors on the order of a few percent.

Participatory budgeting (PB) is already well established in Scotland in the form of community led grant-making yet has recently transformed from a grass-roots activity to a mainstream process or embedded 'policy instrument'. An integral part of this turn is the use of the Consul digital platform as the primary means of citizen participation. Using a mixed method approach, this ongoing research paper explores how each of the 32 local authorities that make up Scotland utilise the Consul platform to engage their citizens in the PB process and how they then make sense of citizens' contributions. In particular, we focus on whether natural language processing (NLP) tools can facilitate both citizen engagement, and the processes by which citizens' contributions are analysed and translated into policies., Comment: 6 pages, presented in GoodIT 2021 Conference

The COVID-19 pandemic is a global event, but what became apparent almost immediately was that while the virus seems indiscriminate, vulnerability and the capacity to mitigate its impact are not spread equally, either between or within countries. Years of austere neoliberalism in Europe have exacerbated inequality and precarity, acting as a 'pre-existing condition' onto which the virus has now landed. The question we ask is: when the pandemic subsides, can the underlying conditions of contemporary neoliberalism remain? And what may replace it?

Botulinum neurotoxins (BoNTs) can be used therapeutically to treat a wide range of neuromuscular and neurological conditions. A collection of natural BoNT variants exists which can be classified into serologically distinct serotypes (BoNT/B), and further divided into subtypes (BoNT/B1, B2, …). BoNT subtypes share a high degree of sequence identity within the same serotype yet can display large variation in toxicity. One such example is BoNT/B2, which was isolated from Clostridium botulinum strain 111 in a clinical case of botulism, and presents a 10-fold lower toxicity than BoNT/B1. In an effort to understand the molecular mechanisms behind this difference in potency, we here present the crystal structures of BoNT/B2 in complex with the ganglioside receptor GD1a, and with the human synaptotagmin I protein receptor. We show, using receptor-binding assays, that BoNT/B2 has a slightly higher affinity for GD1a than BoNT/B1, and confirm its considerably weaker affinity for its protein receptors. Although the overall receptor-binding mechanism is conserved for both receptors, structural analysis suggests the lower affinity of BoNT/B2 is the result of key substitutions, where hydrophobic interactions important for synaptotagmin-binding are replaced by polar residues. This study provides a template to drive the development of future BoNT therapeutic molecules centered on assessing the natural subtype variations in receptor-binding that appears to be one of the principal stages driving toxicity.

Clostridium botulinumneurotoxins (BoNTs) cause flaccid paralysis through inhibition of acetylcholine release from motor neurons; however, at tiny doses, this property is exploited for use as a therapeutic. Each member of the BoNT family of proteins consists of three distinct domains: a binding domain that targets neuronal cell membranes (H-C), a translocation domain (H-N) and a catalytic domain (LC). Here, we present high-resolution crystal structures of the binding domains of BoNT subtypes/A5 (H-C/A5) and/A6 (H-C/A6). These structures show that the core fold identified in other subtypes is maintained, but with subtle differences at the expected receptor-binding sites.

Modern observational and analytic techniques now enable the direct measurement of star formation histories and the inference of galaxy assembly histories. However, current theoretical predictions of assembly are not ideally suited for direct comparison with such observational data. We therefore extend the work of prior examinations of the contribution of ex-situ stars to the stellar mass budget of simulated galaxies. Our predictions are specifically tailored for direct testing with a new generation of observational techniques by calculating ex-situ fractions as functions of galaxy mass and morphological type, for a range of surface brightnesses. These enable comparison with results from large FoV IFU spectrographs, and increasingly accurate spectral fitting, providing a look-up method for the estimated accreted fraction. We furthermore provide predictions of ex-situ mass fractions as functions of galaxy mass, galactocentric radius and environment. Using $z=0$ snapshots from the 100cMpc$^3$ and 25cMpc$^3$ EAGLE simulations we corroborate the findings of prior studies, finding that ex-situ fraction increases with stellar mass for central and satellite galaxies in a stellar mass range of 2$\times$10$^{7}$ - 1.9$\times$10$^{12}$ M$_{\odot}$. For those galaxies of mass M$_*$>5$\times$10$^{8}$M$_{\odot}$, we find that the total ex-situ mass fraction is greater for more extended galaxies at fixed mass. When categorising satellite galaxies by their parent group/cluster halo mass we find that the ex-situ fraction decreases with increasing parent halo mass at fixed galaxy mass. This apparently counter-intuitive result may be the result of high passing velocities within large cluster halos inhibiting efficient accretion onto individual galaxies., Comment: 16 pages, 11 figures. Accepted for publication in MNRAS

We examine the influence of dark matter halo assembly on the evolution of a simulated $\sim L^\star$ galaxy. Starting from a zoom-in simulation of a star-forming galaxy evolved with the EAGLE galaxy formation model, we use the genetic modification technique to create a pair of complementary assembly histories: one in which the halo assembles later than in the unmodified case, and one in which it assembles earlier. Delayed assembly leads to the galaxy exhibiting a greater present-day star formation rate than its unmodified counterpart, whilst in the accelerated case the galaxy quenches at $z\simeq 1$, and becomes spheroidal. We simulate each assembly history nine times, adopting different seeds for the random number generator used by EAGLE's stochastic subgrid implementations of star formation and feedback. The systematic changes driven by differences in assembly history are significantly stronger than the random scatter induced by this stochasticity. The sensitivity of $\sim L^\star$ galaxy evolution to dark matter halo assembly follows from the close coupling of the growth histories of the central black hole (BH) and the halo, such that earlier assembly fosters the formation of a more massive BH, and more efficient expulsion of circumgalactic gas. In response to this expulsion, the circumgalactic medium reconfigures at a lower density, extending its cooling time and thus inhibiting the replenishment of the interstellar medium. Our results indicate that halo assembly history significantly influences the evolution of $\sim L^\star$ central galaxies, and that the expulsion of circumgalactic gas is a crucial step in quenching them., Comment: 19 pages, 11 figures, accepted for publication in MNRAS. High resolution images and movies are available at https://www.astro.ljmu.ac.uk/~arijdav1/gm_paper.html

Context: Dust in late-type galaxies in the local Universe is responsible for absorbing approximately one third of the energy emitted by stars. It is often assumed that dust heating is mainly attributable to the absorption of UV and optical photons emitted by the youngest (<= 100 Myr) stars. Consequently, thermal re-emission by dust at FIR wavelengths is often linked to the star-formation activity of a galaxy. However, several studies argue that the contribution to dust heating by much older stars might be more significant. Advances in radiation transfer (RT) simulations finally allow us to actually quantify the heating mechanisms of diffuse dust by the stellar radiation field. Aims: As one of the main goals in the DustPedia project, we have constructed detailed 3D stellar and dust RT models for nearby galaxies. We analyse the contribution of the different stellar populations to the dust heating in four face-on barred galaxies: NGC1365, M83, M95, and M100. We aim to quantify the fraction directly related to young stars, both globally and on local scales, and to assess the influence of the bar on the heating fraction. Results: We derive global attenuation laws for each galaxy and confirm that galaxies of high sSFR have shallower attenuation curves and weaker UV bumps. On average, 36.5% of the bolometric luminosity is absorbed by dust. We report a clear effect of the bar structure on the radial profiles of the dust-heating fraction by the young stars, and the dust temperature. We find that the young stars are the main contributors to the dust heating, donating, on average ~59% of their luminosity to this purpose throughout the galaxy. This dust-heating fraction drops to ~53% in the bar region and ~38% in the bulge region where the old stars are the dominant contributors to the dust heating. We also find a strong link between the heating fraction by the young stars and the sSFR.

Interstellar dust absorbs stellar light very efficiently and thus shapes the energetic output of galaxies. Studying the impact of different stellar populations on the dust heating remains hard because it requires decoupling the relative geometry of stars and dust, and involves complex processes as scattering and non-local dust heating. We aim to constrain the relative distribution of dust and stellar populations in the spiral galaxy M81 and create a realistic model of the radiation field that describes the observations. Investigating the dust-starlight interaction on local scales, we want to quantify the contribution of young and old stellar populations to the dust heating. We aim to standardise the setup and model selection of such inverse radiative transfer simulations so this can be used for comparable modelling of other nearby galaxies. We present a semi-automated radiative transfer modelling pipeline that implements the necessary steps such as the geometric model construction and the normalisation of the components through an optimisation routine. We use the Monte Carlo radiative transfer code SKIRT to calculate a self-consistent, panchromatic model of the interstellar radiation field. By looking at different stellar populations independently, we can quantify to what extent different stellar age populations contribute to the dust heating. Our method takes into account the effects of non-local heating. We obtain a realistic 3D radiative transfer model of the face-on galaxy M81. We find that only 50.2\% of the dust heating can be attributed to young stellar populations. We confirm a tight correlation between the specific star formation rate and the heating fraction by young stellar populations, both in sky projection and in 3D, also found for radiative transfer models of M31 and M51. We conclude that... (abridged), Comment: 23 pages, 16 figures, accepted for publication in A&A

In order to perform Bayesian parameter estimation to infer the source properties of gravitational waves from compact binary coalescences (CBCs), the noise characteristics of the detector must be understood. It is typically assumed that the detector noise is stationary and Gaussian, characterized by a power spectral density (PSD) that is measured with infinite precision. We present a new method to incorporate the uncertainty in the power spectral density estimation into the Bayesian inference of the binary source parameters and apply it to the first 11 CBC detections reported by the LIGO- Virgo Collaboration. We find that incorporating the PSD uncertainty only leads to variations in the positions and widths of the binary parameter posteriors on the order of a few percent. Our results are publicly available for download on git [1].